Forest Sciences Commons^™

Manipulating Genome Of Diploid Potato Inbred Line Solanum Chacoense M6 Using Selectable Marker Gene, Sarbesh Das Dangol, İlknur Yel, Mehmet Emi̇n Çalişkan, Allah Bakhsh

Turkish Journal of Agriculture and Forestry

The development of transgenic potatoes is imperative to investigate various gene functions as well as to develop robust potato varieties resistant to different biotic and abiotic stresses. Directing a potato breeding program from cultivated tetraploids to self-compatible diploid lines will be highly helpful for potato breeders. However, diploid potatoes are considered recalcitrant to regeneration. We aimed to develop a protocol for the transformation of diploid Solanum chacoense M6 potatoes using leaf, internodes, and microtubers as the explants. Agrobacterium tumefaciens strain GV2260 harboring pBIN19 expression vector containing gusA gene (interrupted by an intron to induce expression from eukaryotic cells) was used …