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Full-Text Articles in Forest Sciences
Isolation And Functional Characterization Of Two Rapeseed Genes Orthologous To Arabidopsis Thaliana Phenylalanine Ammonia-Lyase 2, Yu Ni, Yan-Zhen Dong, Guan-Rong Li, Bo Lei, You-Rong Chai, Jia-Na Li
Isolation And Functional Characterization Of Two Rapeseed Genes Orthologous To Arabidopsis Thaliana Phenylalanine Ammonia-Lyase 2, Yu Ni, Yan-Zhen Dong, Guan-Rong Li, Bo Lei, You-Rong Chai, Jia-Na Li
Turkish Journal of Agriculture and Forestry
Two rapeseed (Brassica napus L.) phenylalanine ammonia-lyase 2 genes (BnPAL2-1 and BnPAL2-2) were isolated. The full-length cDNAs of BnPAL2-1 and BnPAL2-2 are 2422 bp and 2421 bp, with corresponding genomic sequences of 2735 bp and 2742 bp, respectively. Clues from NCBI BLAST, multi-alignment, the phylogenetic tree, conserved domains, preferential amino acids, and tertiary structures all indicated that they are orthologs of Arabidopsis thaliana PAL2 (AtPAL2). The purified His-tagged BnPAL2-1 and BnPAL2-2 proteins, expressed in Escherichia coli, were both active for PAL catabolism, but BnPAL2-2 was much more active than BnPAL2-1. Southern blot analysis showed that the BnPAL2 subfamily might contain …