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Host-Specific Toxin Production By The Tomato Target Leaf Spot Pathogen Corynespora Cassiicola, Şener Kurt
Host-Specific Toxin Production By The Tomato Target Leaf Spot Pathogen Corynespora Cassiicola, Şener Kurt
Turkish Journal of Agriculture and Forestry
The culture filtrate (CF) of Corynespora cassiicola, the causal agent of target leaf spot of tomato, produced visible small, pinpoint, water-soaked lesions on the lower surface of wounded, detached leaves after incubation for 48 h. The extraction, isolation and purification of C. cassiicola tomato (CCT) toxin was achieved by anion exchange and gel filtration chromatography. Treatments of the CF with Diaion HP-20 resin and Cosmosil C18-OPN gel were found to be highly effective. The toxin purified partially by cosmosil gel filtration was highly active against the tomato cultivar Momotaro used in the bioassay test, and the fraction eluted with 50% …