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Full-Text Articles in Forest Sciences
In Situ Localisation Of Axi 1 (Auxin Independent) Gene In Rhizobium Leguminosarum Inoculated Root Tips Of Pea Plants, Yeşi̇m Yalçin Mendi̇, Katharina Pawlowski, Seli̇m Çeti̇ner
In Situ Localisation Of Axi 1 (Auxin Independent) Gene In Rhizobium Leguminosarum Inoculated Root Tips Of Pea Plants, Yeşi̇m Yalçin Mendi̇, Katharina Pawlowski, Seli̇m Çeti̇ner
Turkish Journal of Agriculture and Forestry
Axi 1 is the only characterized auxin independent gene in tobacco plants. In this study, the effect of axi 1 gene on cortical cell division during different stages of nitrogen fixation and on the role of polar auxin transport were investigated. Root and nodule samples were taken from pea seedlings 24 hours, 48 hours, 6 days and 10 days after inoculation with R. leguminosarum PRE strain. Using in situ hybridisation, the location of the axi 1 gene expression in root and nodule tissues was identified. The results showed that in root tissues inoculated with Rhizobia, the axi 1 gene is …
Isolation And Characterization Of Sequences Homologous To The Tobacco Clone Axi 1 (Auxin Independent) From A Vicia Sativa Nodule Cdna Library, Yeşi̇m Yalçin Mendi̇, Seli̇m Çeti̇ner, Ton Bisseling
Isolation And Characterization Of Sequences Homologous To The Tobacco Clone Axi 1 (Auxin Independent) From A Vicia Sativa Nodule Cdna Library, Yeşi̇m Yalçin Mendi̇, Seli̇m Çeti̇ner, Ton Bisseling
Turkish Journal of Agriculture and Forestry
In this research, partial nucleotide sequences of the axi 1 gene, which is related to auxin perception and transduction, isolated from Vicia sativa using cDNA library screening were investigated. Four V. sativa cDNA clones representing homologous of the tobacco axi 1 (auxin independent) cDNA clone were isolated and characterized. With sequence analysis two different clones were observed, called 10/3a and 16/3b. Sequences about 500 bp from the 5' end and 450 bp from the 3' end were determined from the 10/3a clone. The sequences from the 5' end of cDNA clone 10/3a shared about 58 % similarity at the nucleotide …