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Fate Of Β-Lactoglobulin, Α-Lactalbumin, And Casein Proteins In Ultrafiltered Concentrated Milk After Ultra-High Temperature Processing, Mark Christopher Alleyne
Fate Of Β-Lactoglobulin, Α-Lactalbumin, And Casein Proteins In Ultrafiltered Concentrated Milk After Ultra-High Temperature Processing, Mark Christopher Alleyne
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The problem of age gelation in ultra-high temperature (UHT) sterilized milk retentate (ultrafiltered 3x concentrated) is investigated in this work. Transmission electron microscopy (TEM), utilizing the microcube encapsulation technique and protocols for immunolocalization of milk proteins, provides insight into the phenomenon of age gelation of UHT-sterilized, ultrafiltered (UF) milk retentate. Primary antibodies (specific for the native as well as the complexed forms of milk proteins) and secondary antibodies (conjugated to gold probes) are used to elucidate the positions of the milk proteins in various samples of milk from the stage of milking through UHT sterilization and storage for 12 months, …