Food Processing Commons^™

Determination Of Soybean And Wheat Allergens In Food By Dual Real-Time Fluorescence Pcr, Jin Ping, Ding Hong-Liu, Zhang Min, Jin Xiao-Hong

Food and Machinery

Objective: A multiplex fluorescence PCR method based on TaqMan probe was developed for simultaneous detection of soybean and wheat allergens in food. Methods: TaqMan probe dual PCR was designed and established based on wheat gliadin gene and soybean lectin gene, respectively. The soybean Lectin gene was detected with FAM marker, and the wheat gliadin gene was detected with HEX marker. Moreover, the eukaryotic 18S rRNA was used as internal reference gene to ensure the effectiveness of the detection system. Results: The multiplex fluorescence PCR method for detection of soybean and wheat allergens created in this …

Rapid Detection Of Alternaria Monomethyl Ether In Wheat By High Performance Liquid Chromatography, Xu Qun-Ying, Zhai Yu-Heng

Food and Machinery

In this study, a high-performance liquid chromatography (HPLC) method for the efficient detection of alternariolmethyl ether (AME) in wheat was developed. The sample was extracted with acetonitrile/ methanol/ water (volume ratio 45/10/45), shaken at 30 ℃ for 30 minutes, and purified by Bond Elut Plexa solid-phase extraction cartridge. Then the sample was detected with a Variable wavelength detector. In this method, the limit of detection (LOD) of AME was 2.52 μg/kg, and the limit of quantification (LOQ) was 8.40 μg/kg, and the linear range was 0.25~2.00 μg/mL. The concentration of AME recovery was 80.1%~88.3%, the intra-day precision and inter-day precision …

Wheat Payments And Protein Content, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

In recent years there has been increasing support from within the Australian wheat industry for the principal of wheat growers recieving payment for their grain according to market value.

Individual loads are now tested for quality characteristics such as hectolitre weight, unmillable material and moisture content.

At present, technology is not sufficiently advanced to enable rapid and accurate analysis of individual loads for all important quality factors.

A more practical system of quality assessment would be one based on allowances for various basic measures (such as hectolitre weight, unmillable material and moisture content), variety (to allow for genetic differences in …

Insignia Lowers Wheat Quality, J A. Parish, H. M. Fisher

Journal of the Department of Agriculture, Western Australia, Series 4

Farmers in Western Australia have been advised not to plant Insignia wheat because it has poor grain quality. Many farmers want to know why Insignia is considered poor quality and what harm it could do. This article answers these questions and suggests alternative, better quality wheats for all present situations.