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Influence Of Hierarchical Interfacial Assembly On Lipase Stability And Performance In Deep Eutectic Solvent, Stephanie M. Andler
Influence Of Hierarchical Interfacial Assembly On Lipase Stability And Performance In Deep Eutectic Solvent, Stephanie M. Andler
Masters Theses
Hierarchical systems that integrate nano- and macroscale structural elements can offer enhanced stability over traditional immobilization methods. Microparticles were synthesized using interfacial assembly of lipase with (CLMP-N) and without (CLMP) nanoparticles into a crosslinked polymeric core, to determine the impact of the highly ordered system on lipase stability in extreme environments. Kinetic analysis revealed the macrostructure significantly increases the turnover rate (kcat) following immobilization. The macrostructure also stabilized lipase at neutral and basic pH values, while the nanoparticles influenced stability under acidic pH conditions. A greener solvent, choline chloride and urea, was applied to produce sugar ester …