Food Chemistry Commons^™

Arabinoxylan Structural Profiling Of Cool-Season Pasture Grasses Via High-Performance Anion-Exchange Chromatography With Pulsed Amperometric Detection (Hpaec-Pad) Analysis Of Endoxylanase Digests, Glenna Erin Joyce

Theses and Dissertations--Animal and Food Sciences

Arabinoxylan (AX) is a major structural polysaccharide found in the cell walls of monocots such as cereal grains and pasture grasses. The variety of AX structural components and substitution patterns contribute to AX structural diversity between different monocot species as well as plant tissues.

The rumen is the first digestion site of masticated food material in cattle and provides 70% of energy to host through fermentation of forage. There are many species of pasture grasses that act as a forage source. Differences in AX structure found in these pasture grasses may impact rumen microbial fermentation. Understanding the AX structure of …

Online Ultra-High Performance Liquid Chromatography-Charge Transfer Dissociation-Mass Spectrometry (Uhplc-Ctd-Ms) Of Complex Mixtures Of Oligosaccharides, Praneeth M. Mendis

Graduate Theses, Dissertations, and Problem Reports

Abstract

Online Ultra-High Performance Liquid Chromatography-Charge Transfer Dissociation-Mass Spectrometry (UHPLC-CTD-MS) of Complex Mixtures of Oligosaccharides

Praneeth M. Mendis

Complex carbohydrates make up more than half the biomass on earth, and they are crucial to a wide range of processes in living organisms. When polysaccharides and glycans are enzymatically digested into manageable units called oligosaccharides, they typically consist of 2-20 linear or branched sugar units. The detailed structural analysis of oligosaccharides assists in the identification of structural and chemical properties of the polymers from which they derive. Tandem mass spectrometry (MS/MS) is a key technique used in oligosaccharides structural characterization. Within …