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Full-Text Articles in Food Science
Determination Of Soybean And Wheat Allergens In Food By Dual Real-Time Fluorescence Pcr, Jin Ping, Ding Hong-Liu, Zhang Min, Jin Xiao-Hong
Determination Of Soybean And Wheat Allergens In Food By Dual Real-Time Fluorescence Pcr, Jin Ping, Ding Hong-Liu, Zhang Min, Jin Xiao-Hong
Food and Machinery
Objective: A multiplex fluorescence PCR method based on TaqMan probe was developed for simultaneous detection of soybean and wheat allergens in food. Methods: TaqMan probe dual PCR was designed and established based on wheat gliadin gene and soybean lectin gene, respectively. The soybean Lectin gene was detected with FAM marker, and the wheat gliadin gene was detected with HEX marker. Moreover, the eukaryotic 18S rRNA was used as internal reference gene to ensure the effectiveness of the detection system. Results: The multiplex fluorescence PCR method for detection of soybean and wheat allergens created in this …
Rapid Detection Of Alternaria Monomethyl Ether In Wheat By High Performance Liquid Chromatography, Xu Qun-Ying, Zhai Yu-Heng
Rapid Detection Of Alternaria Monomethyl Ether In Wheat By High Performance Liquid Chromatography, Xu Qun-Ying, Zhai Yu-Heng
Food and Machinery
In this study, a high-performance liquid chromatography (HPLC) method for the efficient detection of alternariolmethyl ether (AME) in wheat was developed. The sample was extracted with acetonitrile/ methanol/ water (volume ratio 45/10/45), shaken at 30 ℃ for 30 minutes, and purified by Bond Elut Plexa solid-phase extraction cartridge. Then the sample was detected with a Variable wavelength detector. In this method, the limit of detection (LOD) of AME was 2.52 μg/kg, and the limit of quantification (LOQ) was 8.40 μg/kg, and the linear range was 0.25~2.00 μg/mL. The concentration of AME recovery was 80.1%~88.3%, the intra-day precision and inter-day precision …