Food Science Commons^™

Presence, Co-Occurrence, And Daily Intake Estimates Of Aflatoxins And Fumonisins In Maize Consumed In Food-Insecure Regions Of Western Honduras, Luis Sabillón, Jackeline Alvarado, Alejandra Leiva, Rodrigo Mendoza, Raúl Espinal, John F. Leslie, Andreia Bianchini

Department of Food Science and Technology: Faculty Publications

Foodborne mycotoxins are a significant food safety risk in developing countries. Our objective was to determine the occurrence of and exposure levels to aflatoxins (AFs) and fumonisins (FBs) in maize intended for human and animal consumption in food-insecure regions of western Honduras. Total AFs and FBs were quantified with a monoclonal antibody-based affinity spectrofluorimetric method. FBs were detected in 614/631 samples of maize destined for human consumption at 0.3 to 41 mg/kg (mean, 2.7 mg/kg). Of the 614 positive samples, 147 had FB levels exceeding the U.S. Food and Drug Administration (FDA) advisory threshold of 4.0 mg/kg. AFs were detected …

Effect Of High-Pressure Processing On The Microbial Load And Functionality Of Sugar-Cookie Dough, Luis Sabillón, Jayne E. Stratton, Devin Rose, Kent M. Eskridge, Andreia Bianchini

Department of Food Science and Technology: Faculty Publications

Background and objectives: Refrigerated dough products have the potential to be a safety hazard to consumers because they could be consumed raw or undercooked. The objectives of this study were designed to evaluate the microbial and functionality changes in high pressured sugar-cookie dough as a function of a_w (0.80–0.87), pressure level (100–600 MPa), and holding time (1–6 min).

Findings: Endogenous microbial populations were marginally reduced (0.2–0.5 log CFU/g) by pressure treatments. However, treating the dough at 600 MPa for 6 min significantly reduced counts of inoculated Escherichia coli by as much as 2.0 log CFU/g. Increasing the a_{w …}

Heuristic And Hierarchical-Based Population Mining Of Salmonella Enterica Lineage I Pan-Genomes As A Platform To Enhance Food Safety, Joao Carlos Gomes-Neto, Natasha Pavlovikj, Carmen Cano, Baha Abdalhamid, Gabriel Asad Al-Ghalith, John Dustin Loy, Dan Knights, Peter C. Iwen, Byron D. Chaves, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

The recent incorporation of bacterial whole-genome sequencing (WGS) into Public Health laboratories has enhanced foodborne outbreak detection and source attribution. As a result, large volumes of publicly available datasets can be used to study the biology of foodborne pathogen populations at an unprecedented scale. To demonstrate the application of a heuristic and agnostic hierarchical population structure guided pan-genome enrichment analysis (PANGEA), we used populations of S. enterica lineage I to achieve two main objectives: (i) show how hierarchical population inquiry at different scales of resolution can enhance ecological and epidemiological inquiries; and (ii) identify population-specific inferable traits that could provide …

Research Tools And Their Uses For Determining The Thermal Inactivation Kinetics Of Salmonella In Low-Moisture Foods, Soon Kiat Lau

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The reputation of low-moisture foods as safe foods has been crumbling over the past decade due to repeated involvement in foodborne illness outbreaks. Although various pasteurization technologies exist, a majority are thermal processes and have not been well-characterized for pasteurizing low-moisture foods. In addition, the nature of a low-moisture food matrix introduces various experimental complications that are not encountered in high-moisture foods. In this dissertation, the development, building instructions, and characterization of various open source tools for studying the inactivation kinetics of microorganisms in low-moisture foods are described. The first tool is the TDT Sandwich, a dry heating device for …

Tdt Sandwich: An Open Source Dry Heat System For Characterizing The Thermal Resistance Of Microorganisms, Soon Kiat Lau, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

The determination of the thermal death kinetics of microorganisms has traditionally been performed with liquid baths which have some disadvantages such as liquid spillage and liquid infiltration into samples. The TDT Sandwich was developed as a free, open source alternative that utilizes dry heat. The system is capable of heating samples up to 140C and maintaining it within 0.2C of the target temperature. Other features of the TDT Sandwich include adjustable heating rates up to approximately 100C/min, real-time display and recording of temperature readings at a nominal rate of 5 Hz, an optional thermocouple for acquiring temperature of samples, built-in …

Mind The Gaps: How Can Food Safety Gaps Be Addressed In Developing Nations?, Jessie L. Vipham, Byron D. Chaves, Valentina Trinetta

Department of Food Science and Technology: Faculty Publications

As global food systems integrate, to meet the demands of a growing global population, safe and healthy agriculture value-chains will be essential to maintaining public health worldwide. In many ways, the current global food production landscape is made up of “mice and men.” Meaning that the food sector within and across most countries includes both large-scale corporate agriculture production and varying degrees of small-scale agriculture production; the variation being the interpretation of small scale, which is highly dependent upon the country. This structure presents challenges for the creation of effective food safety systems in many countries, and can contribute to …

Biosafety: Evaluation And Regulation Of Genetically Modified (Gm) Crops In The United States, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

This review of the safety assessment of genetically modified (GM) crops is focused primarily on the process and progress in the United States (US). It reviews the development of the safety evaluation process from the Asilomar conference in 1975 considering issues relevant to recombinant DNA technology, to discussions between the US government, academic and industrial scientists between 1984 and 1994 when the first GM crops were being field tested and evaluated commercial release for food and feed production. International guidelines were also reviewed for consistency with the US system. The overall process includes consideration of information relating to history of …

Suggested Improvements For The Allergenicity Assessment Of Genetically Modified Plants Used In Foods, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Genetically modified (GM) plants are increasingly used for food production and industrial applications. As the global population has surpassed 7 billion and per capita consumption rises, food production is challenged by loss of arable land, changing weather patterns, and evolving plant pests and disease. Previous gains in quantity and quality relied on natural or artificial breeding, random mutagenesis, increased pesticide and fertilizer use, and improved farming techniques, all without a formal safety evaluation. However, the direct introduction of novel genes raised questions regarding safety that are being addressed by an evaluation process that considers potential increases in the allergenicity, toxicity, …