Food Science Commons^™

Comparison Of Methods For Defatted Human Milk And Nutrient Composition: An Experimental Study, Kristin Cheng, Gina O'Toole, Stephanie Chang, Richard Chang, John Miklavcic

Food Science Faculty Articles and Research

Background

Human milk is considered the gold standard for infant nutrition; however, it is often discontinued in chylothorax and severe very long-chain acyl-CoA dehydrogenase (VLCAD) deficiency. As an alternative to complete human milk restriction, defatted human milk may be fed, though it may not fully support optimal infant growth and development. This study aimed to assess the micronutrient profile of defatted human milk.

Methods

Human milk was obtained from UCSD Human Milk Research Biorepository (San Diego, CA). Thirty samples representing 10 independent expressions in the morning, afternoon, and evening were pooled for analysis. Fat removal was performed by centrifugation at …

Exploring The Influence Of Environmental Motivations On Consumer Attitudes And Purchase Intentions Toward Upcycled Foods, Peng Lu, Jennifer Waldeck, Keith Weber, Hualu Zhou

Communication Faculty Articles and Research

Food loss and waste (FLW) present pressing environmental and economic challenges. Upcycling technologies provide a sustainable pathway by transforming food by-products into value-added products; however, consumer acceptance remains a critical barrier. Guided by Self-Determination Theory (SDT), this study investigates how environmental motivations influence consumers’ attitudes and purchase intentions toward milk enriched with upcycled peanut skins. Data were collected from a nationally representative sample of 1488 U.S. adults. Structural equation modeling (SEM) was employed to test hypothesized relationships among motivational constructs, and cluster analysis was used to identify consumer segments based on motivational profiles. The results show that intrinsic motivation and …

Alkaline And Acid Extraction Of An L-Tryptophan–Sunflower Flour Red Colorant: Production, Characterization, And Utilization, Spencer Nguyen, Marissa Wong, Lilian Were Senger

Food Science Faculty Articles and Research

The objective was to produce and characterize a red colorant resulting from interaction of sunflower-derived chlorogenic acid with L-tryptophan/Trp and to evaluate its applications. Yield, intensity, solubility, stability, protein, and metal content of colorants from pH 9 and pH 11 lyophilized sunflower flour- Trp colorants were measured. Red color formation took < 3 h with alkalization and subsequent acidification. The sunflower flour: Trp mixture ratio that produced the greatest redness was 10:2. The highest red pigment was in supernatants and not the precipitates. The red colorant intensity and solubility were highest at neutral pH when compared to acidic pH solutions. The color stability of the new red colorant in icing and refrigerated whole milk matrices, when assessed weekly for a month, showed no color change in refrigerated milk. After 30 min of incubation at 63 °C, pinkness in milk remained unchanged or increased. The pink color, however, faded in icing over time due to exposure to light. The present study established a new natural red colorant developed from sunflower protein-Trp mixtures at alkaline pH, as well as its application in icings and whole milk for commercial strawberry milk products. This colorant could serve as a replacement for the synthetic petroleum-based Red 3.

From Waste To Sustainability: Exploring Consumer Purchase Intentions Of Milk Enriched With Upcycled Peanut Skins, Peng Lu, Fan Xu, Jennifer Waldeck, Keith Weber, Hualu Zhou

Communication Faculty Articles and Research

Highlights

• National survey investigated consumer acceptance of milk enriched with upcycled peanut skins.
• Developed a conceptual model based on Technology Acceptance Model.
• Identified that consumer attitudes and emotions are essential in the purchase intentions of upcycled foods.
• Identified social influence as a determinator of consumers' attitudes and purchase intentions.
• Inform and advance research, practice, and policy that promotes upcycled food products.

The Innovative Food Choice Map As A Bottom-Up Mixed-Methods Approach To Studying Food Choice Motivations, Uyen Thuy Xuan Phan, Edgar Chambers Iv

Food Science Faculty Articles and Research

This study presents the innovative Food Choice Map (iFCM) as a methodological innovation for investigating food choice motivations using an integrated qualitative and quantitative approach. One hundred in-depth interviews were conducted with adults living in the United States, during which participants constructed individualized visual maps of their typical weekly diet. These maps documented specific foods and beverages, eating occasions, frequencies of consumption, and self-articulated motivations for each choice. Using a bottom-up framework grounded in real consumption events, qualitative data were inductively coded into fifteen motivation constructs, which were subsequently analyzed using Correspondence Analysis to demonstrate how motivational patterns can be …

Comparative Effects Of Gamma Irradiation And Chemical Treatments On Superficial Scald And Quality Of ‘Granny Smith’ Apples, Francisco E. Loayza, Michelle Tu, Hagop S. Atamian, Anuradha Prakash

Food Science Faculty Articles and Research

Fresh produce is highly perishable, necessitating various postharvest treatments to minimize quality loss, extend shelf-life, and facilitate international trade. This study investigated the potential of a single low-dose gamma irradiation treatment as an alternative to commercially used chemical treatments for protecting ‘Granny Smith’ apples against insect pests and postharvest disorders. ‘Granny Smith’ apples harvested in 2019 and 2020 were treated with gamma irradiation at doses of 240 or 860 Gray (Gy) or with commercial chemical applications of 1-methylcyclopropene (1-MCP), diphenylamine (DPA), or methyl bromide (MeBr). The impact on fruit quality, physiology, and the development of postharvest disorders was evaluated after …

Characterization Of Free Oxylipins In Extracellular Vesicles From Bovine Milk, Kai Filio

Food Science (MS) Theses

Background. Extracellular vesicles (EVs) are membrane-bound nanostructures released by human and bovine mammary epithelial cells that carry bioactive lipids and oxidized lipid metabolites. Found in bovine milk, EVs contribute to immune modulation, gut health, and cellular signaling. However, gaps remain in understanding the full composition of milk-derived EVs and how processing alters EV content. This research aimed to characterize the free oxylipin content of EVs from bovine milk treated by pasteurization and homogenization. The hypothesis was that EVs from raw bovine milk would retain higher oxylipin levels than processed milk. Methods. Raw milk was treated by high-temperature short time (HTST), …

Fish-Spec: Fast Identification System For Handheld Spectroscopy And Species Classification, Mitchell Sueker, Nicholas Mackinnon, Gregory Bearman, Amanda Tabb, Diane Kim, Rosalee S. Hellberg, Alireza Akhbardeh, Hamid Reza Marateb, Jianwei Qin, Moon Kim, Fartash Vasefi, Hossein Kashani Zadeh

Food Science Faculty Articles and Research

Accurate fish species identification is critical to prevent mislabeling and fraud in the seafood industry. We present a handheld multi-mode point spectroscopy system that combines fluorescence (365 and 395 nm excitation) and reflectance measurements in the visible to near-infrared (∼350–900 nm) and short-wave infrared (∼900–1700 nm) regions for rapid, non-destructive classification of fish fillets. Tissue spectra were acquired at 25 positions on 68 fillets from 11 species, in both frozen and thawed states. Feature-level fusion across all four modes enabled higher classification accuracy than any single mode alone. A global machine-learning model classified all species with 85 ± 2.8 %, …

Major Drivers Of Consumer Choice Of Dairy Milk And Plant-Based Milk Alternatives In Coffee, Uyen Thuy Xuan Phan, Luu Hoang, Keith Weber, Anuradha Prakash

Food Science Faculty Articles and Research

Coffee shops in the United States typically offer several options of dairy milk and plant-based milk alternatives (PBMA) for espresso-based beverages. Although previous studies have explored consumer preferences for dairy or PBMA in general, none have examined what drives their milk or PBMA choices specifically in the context of coffee shops. This study aimed to identify key decision drivers for the choice of milks in coffee, assess the role of lactose-free preferences, and explore whether offering lactose-free cow milk would increase coffee shop visits. Following a preliminary study using in-depth interviews, an online survey was used to collect data from …

Effective Control Of Tomato Leaf Miner, Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae) Using The Entomopathogenic Fungus, Beauveria Pseudobassiana Rehner & Humber In Commercial High-Arched Plastic Tunnels, Aya Fardoun, Nour Ezzeddine, Hagop S. Atamian, Hana Sobh, Houssam Shaib, Yusuf Abou Jawdah

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background

The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is a highly invasive pest that causes significant damages to tomato crops globally. Its resistance to commonly used chemical insecticides has made management increasingly difficult, prompting the need for sustainable, effective alternatives. This study investigated the efficacy of a Lebanese isolate of Beauveria pseudobassiana as a biological control agent against T. absoluta, compared its performance to Bacillus thuringiensis and conventional pesticide mixtures under both controlled, commercial production conditions

Results

Three preliminary cage experiments were conducted under controlled infestation conditions using larvae reared from field-collected adults. Conidial suspensions of B. pseudobassiana …

Unraveling Crop Nitrogen-Water Dynamics With Hyperspectral-Thermal Sensing In Northern Central Valley, California, Shahryar Fazli, Surendra Maharjan, Wenzhao Li, Joshua B. Fisher, Rejoice Thomas, Fernando Romero Galvan, Gabriela Shirkey, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Ensuring global food security in the face of climate change requires optimizing crop water use and nutrient management. This study investigates the relationship between canopy nitrogen (N) and evapotranspiration (ET) across sunflower, rice, walnut, alfalfa, and plum crops using advanced remote sensing technologies. High-resolution hyperspectral data from NASAs Earth Surface Mineral Dust Source Investigation (EMIT) and thermal multispectral data from the Landsat-based OpenET system were analyzed over 1,135 km² in California. Regression analysis revealed strong spatial association between canopy N and ET for sunflower (R² = 0.82), rice (R² = 0.71), and walnut (R² = 0.68), …

Comparison Of Pcr-Based Methods For The Detection Of Canned Tuna Species, Chloe P. Castanon, Denise Hernandez, Akshay N. Khetrapal, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna …

Comparative Impacts Of Irradiation On Antioxidant Compounds Of Scalded And Non-Scalded Skin Of Granny Smith Apples, Angela Banda

Food Science (MS) Theses

Post-harvest Superficial scald affects the quality of Granny Smith Apples. Oxidative stress develops superficial scald with antioxidant systems mitigating the extent of this damage. This study evaluated the effects of gamma, X-ray, and electron beam irradiation on antioxidants – ascorbic acid, total phenolics, α-tocopherol, carotenoids, chlorophyll, and campesterol – in scalded and non-scalded apple peel tissue. Apples were treated with 0.2-0.77 kGy and stored at 0-1 °C. Irradiation at the applied doses increased scald incidence, suggesting that disruption of antioxidant systems promoted reactive oxygen species accumulation and membrane damage. While certain antioxidants appeared to be upregulated as a stress response, …

Highly Targeted Metabolomics Coupled With Gene Expression Analysis By Rt–Qpcr Improves Beef Separation Based On Grass, Grain, Or Grape Supplemented Diet, Lucas Krusinski, Chloe Castanon, Rosalee S. Hellberg, Isabella C. F. Maciel, Muhammad Ahsin, Stephan Van Vliet, Jason E. Rowntree, Jennifer I. Fenton

Food Science Faculty Articles and Research

The objective of this study was to use a multi-omics (i.e., gene expression quantification, metabolomics, and fatty acid [FA] profiling) approach to separate and authenticate beef from three different dietary groups. In this 2-year study, Red Angus steers (n = 54) were randomly allocated to one of three treatments: (1) complex biodiverse pasture (GRASS), (2) total mixed ration (TMR) in feedlot (GRAIN), or (3) TMR in feedlot supplemented with 5% (dry matter) grapeseed extract for the last 30 days (GRAPE). FAs were measured by gas chromatography-mass spectrometry (GC–MS), secondary metabolites were identified using ultra-high-performance liquid chromatography tandem mass spectrometry …

A Meta-Analysis Of Seafood Species Mislabeling In The United States, Sarah Ahles, Christina A. Mireles Dewitt, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Seafood is vulnerable to mislabeling due to complex global supply chains, varying prices, and the similar appearance of species. While numerous studies have been published on seafood mislabeling, the focus is often on species known to be commonly substituted. Therefore, the overall mislabeling rates of seafood sold in the U.S. remain unknown, especially for the most consumed species. The objective of the current study was to compile the results of seafood mislabeling studies into a single resource to provide informative statistics on U.S. seafood mislabeling. A meta-analysis was conducted on U.S. seafood mislabeling studies that tested commercial samples of bony …

Seasonal Dynamics In Land Surface Temperature In Response To Land Use Land Cover Changes Using Google Earth Engine, Lei Feng, Sajjad Hussain, Narcisa G. Pricope, Sana Arshad, Aqil Tariq, Li Feng, Muhammad Mubeen, Rana Waqar Aslam, Mohammed S. Fnais, Wenzhao Li, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Changes in land use and land cover (LULC) are critical for evaluating global spatiotemporal trends, especially regarding climate change and urbanization. This study investigates the dynamics of Landsat surface temperature (LST) in response to LULC changes and their effects on the seasonal microclimate in Kasur District, Pakistan. Using the Google Earth Engine platform, we employed a random forest algorithm to detect LULC changes (cropland, forest, built-up, fallow, barren, and water) and analyze seasonal spectral indices from Landsat imagery for 1988, 2002, and 2022. Significant LULC changes were observed, including a 9.8% increase in built-up areas, a 4.2% decrease in cropland, …

Use Of A Novel Combination Of Multiplex Pcr And Dna Barcoding In Assessing Authenticity Of Ginseng Products, Diane Y. Kim, Donna Miranda-Romo, Adriana R. Ten Cate, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Ginseng (Panax sp.) is a medicinal plant used for its purported health benefits, primarily in East Asian countries. The COVID-19 pandemic led to a significant increase in sales of dietary supplements, including ginseng supplements, to purportedly support immune health and provide other health benefits. However, this heightened demand has subsequently increased the risk of adulteration in these dietary supplements. This study aimed to determine the efficacy of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing Panax ginseng, Panax quinquefolius, or Panax notoginseng were obtained …

Comparison Of Chlorophyll, Carotenoid, And Alpha-Tocopherol In E-Beam, Gamma, And X-Ray Treated 'Granny Smith' Apples, Manju Meena Vetrivelayutham

Food Science (MS) Theses

This study aimed to investigate the impact of phytosanitary radiation modality on the lipophilic antioxidants in peels of ‘Granny Smith’ apples. Freshly harvested ‘Granny Smith’ apples were treated with gamma (0.2 kGy), X-ray (0.495 kGy), and e-beam (0.77 kGy) radiation and stored in air at 1 ⁰C for up to four months. Non-irradiated apples were used as control fruit. Peels of irradiated and non-irradiated apples were collected and quantified for chlorophyll, carotenoids, alpha-tocopherol, and antioxidant capacity. The results indicated that gamma irradiation significantly reduced the chlorophyll content by 76% but did not impact X-ray and e-beam-treated apple peels, even though …

Thiol Dough Conditioners Impact On The Formation Of Advanced Glycation End-Products In Bagels Containing Sunflower Seed Flour, Alton Chambers

Food Science (MS) Theses

Baking bagels can increase advanced glycation end products (AGEs), which are linked to increased metabolic and chronic diseases. This research correlated color and the proximate composition with fluorescent AGEs in bagels containing 0-20% sunflower flour (SFF) and 0-90 ppm L-cysteine (Cys) or Glutathione (GSH). No significant differences were observed in the moisture and lipid content for the bagel crust and crumb. Carbohydrate content decreased, while protein and ash content significantly increased when the SFF increased from 0 to 20 % SFF. The pH of the crust and crumb increased from 5.27 to 6.01 and 5.33 to 6.09, respectively, when sunflower …

Impact Of Phytosanitary Irradiation On Polar Antioxidants In Peels Of ‘Granny Smith’ Apples, Sneha Anna Alexander

Food Science (MS) Theses

The ‘Granny Smith’ apple (Malus domestica Borkh), known for its bright green peel and tart flavor, is a significant cultivar in the United States. Due to increasing limitations and restrictions on chemical fumigants and other treatments, ionizing radiation is gaining popularity as a phytosanitary treatment to prevent the spread of pests while preserving food quality. This study aimed to evaluate the effects of gamma, e-beam, and X-ray irradiation on phenolic compounds, vitamin C content, and total antioxidant capacity in ‘Granny Smith’ apple peels. The results indicated that there were no significant changes in vitamin C content immediately following …

Characterizing Oxylipins In Human Milk Extracellular Vesicles, Priscila Herrera

Food Science (MS) Theses

Extracellular vesicles (EVs) are non-replicating lipid bilayer membrane-bound particle formed by a parent cell and secreted into the extracellular environment. EVs in human milk are bioavailable and influence physiologic pathways that may contribute to newborn growth and development. There is interest in the oxylipin content of EVs that may contribute to the bioavailability of EVs. This research aims to characterize the oxylipin content of human milk EVs. Centrifugation and precipitation were used to isolate EVs from human milk (n = 20). The Folch method was used to extract lipids, and liquid chromatography-mass spectrometry (LC– MS) was used to qualify and …

Twenty-Three Years Of Pcr-Based Seafood Authentication Assay Development: What Have We Learned?, Maleeka Singh, Robert G. Young, Rosalee S. Hellberg, Robert H. Hanner, Maria G. Corradini, Jeffrey M. Farber

Food Science Faculty Articles and Research

Seafood is a prime target for fraudulent activities due to the complexity of its supply chain, high demand, and difficult discrimination among species once morphological characteristics are removed. Instances of seafood fraud are expected to increase due to growing demand. This manuscript reviews the application of DNA-based methods for commercial fish authentication and identification from 2000 to 2023. It explores (1) the most common types of commercial fish used in assay development, (2) the type of method used, (3) the gene region most often targeted, (4) provides a case study of currently published assays or primer-probe pairs used for DNA …

Twenty-Three Years Of Pcr-Based Seafood Authentication Assay Development: What Have We Learned?, Maleeka Singh, Robert G. Young, Rosalee S. Hellberg, Robert H. Hanner, Maria G. Corradini, Jeffrey M. Garber

Food Science Faculty Articles and Research

Seafood is a prime target for fraudulent activities due to the complexity of its supply chain, high demand, and difficult discrimination among species once morphological characteristics are removed. Instances of seafood fraud are expected to increase due to growing demand. This manuscript reviews the application of DNA-based methods for commercial fish authentication and identification from 2000 to 2023. It explores (1) the most common types of commercial fish used in assay development, (2) the type of method used, (3) the gene region most often targeted, (4) provides a case study of currently published assays or primer-probe pairs used for DNA …

Socioeconomic Constraints On Low-Income Individuals' Perceptions Toward Food Safety, Uyen Thuy Xuan Phan, Cuc Thuy Thi Tran, Nguyet Minh Thi Nguyen

Food Science Faculty Articles and Research

Food safety has emerged as a critical concern in Vietnam, posing a significant threat to public health due to the prevalence of unsafe foods in the market. Among those most vulnerable to contaminated foods, low-income individuals are significantly affected. This study aimed to investigate the perceptions of food safety among this demographic, utilizing qualitative and quantitative approaches, including focus groups, survey questionnaires, and behavioral experiments using steamed bun as a stimulus. Participants were generally from low-income classes with certain variations in household monthly income. Results from focus groups and the survey highlighted that the factors influencing food purchases included perception …

Use Of A Novel Combination Of Multiplex Pcr And Dna Barcoding In Assessing Authenticity Of Ginseng Products, Diane Kim

Food Science (MS) Theses

Ginseng (Panax sp.) is a medicinal plant root used for its purported health benefits, primarily in East Asian countries. The COVID-19 pandemic led to a significant increase in sales of dietary supplements, including ginseng supplements, to purportedly support immune health and provide other health benefits. However, this heightened demand has subsequently increased the risk of adulteration in these dietary supplements. The objective of this study was to determine the efficacy of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing either Panax ginseng, Panax quinquefolius, or …

Optimization Of Pre-Enrichment And Screening Methods For The Detection Of Salmonella Enterica In Meat Analog Products, Kylie Sacapano

Food Science (MS) Theses

With the growing demand and variety of meat analogs on the commercial market, there is a need to revisit the methodology for the detection of foodborne pathogens in these products. The current Food and Drug Administration (FDA) regulatory methods for Salmonella enterica detection in meat analogs follow the Bacteriological Analytical Manual (BAM) and are time-consuming and costly, requiring pre-enrichment using lactose broth (LB) with a surfactant (e.g., Triton X-100). Therefore, this study aimed to optimize pre-enrichment and screening methods for the detection of Salmonella in meat analogs by comparing the current FDA reference methods to updated techniques. To achieve this, …

A Meta-Analysis Of Seafood Species Mislabeling Rates In The United States, Sarah Ahles

Food Science (MS) Theses

Seafood is vulnerable to species mislabeling due to factors such as complex global supply chains, varying prices, and similar appearance of species. Numerous studies have been published reporting a range of mislabeling rates for various forms of seafood. However, these studies oftentimes focus on lesser-consumed species that are vulnerable to species mislabeling. As a result, the overall mislabeling rate of commercially sold seafood in the U.S. remains unknown, especially for the most consumed species. Thus, the objective of the current study was to compile the results of seafood mislabeling studies into a single resource to provide informative statistics on U.S. …

Chlorogenic And Caffeic Acids’ Impact On Formation Of Fluorescent And Non-Fluorescent Advanced Glycation End-Products As A Function Of Temperature, Christianne Yapor

Food Science (MS) Theses

The glycation of amino acids with carbonyl groups of reducing sugars produces advanced glycation end-products (AGEs). These AGEs can be harmful to health over time. The research objective was to compare the effects of 0-1.00 mM chlorogenic acid (CGA) and caffeic acid (CA) on AGEs formed in glucose-lysine solutions at 25 °C, 63 °C and 100 °C for 30 and 60 minutes by measuring fluorescent AGEs: vesperlysine A and B, vesperlysine C, and total AGEs, and non- fluorescent carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL) with LC-MS. Chlorogenic acid inhibited the formation of AGEs to a greater extent compared to CA at …

Inactivation Of Escherichia Coli, Salmonella Enterica, And Listeria Monocytogenes Using The Contamination Sanitization Inspection And Disinfection (Csi-D) Device, Jennifer Mccoy Sanders, Vanessa Alarcon, Grace Marquis, Amanda Tabb, Jo Ann Van Kessel, Jakeitha Sonnier, Brad J. Haley, Insuck Baek, Jianwei Qin, Moon Kim, Fartash Vasefi, Stanislav Sokolov, Rosalee S. Hellberg

Food Science Faculty Articles and Research

The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging system designed to disinfect food contact surfaces using ultraviolet-C (UVC) illumination. This study aimed to determine the optimal CSI-D parameters (i.e., UVC exposure time and intensity) for the inactivation of the following foodborne bacteria plated on non-selective media: generic Escherichia coli (indicator organism) and the pathogens enterohemorrhagic E. coli, enterotoxigenic E. coli, Salmonella enterica, and Listeria monocytogenes. Each bacterial strain was spread-plated on non-selective agar and exposed to high-intensity (10 mW/cm²) or low-intensity (5 mW/cm²) UVC for 1–5 s. Control …

Evaluation Of Yield And Physicochemical Quality Of Pentadesma Butyracea Butter Obtained By Different Traditional Extraction Methods In Ghana, Josephine Akuba Timtey, Francis Alemawor, William Otoo Ellis, Jacob K. Agbenorhevi, Nana Baah Pepra-Ameyaw

Food Science Faculty Articles and Research

Pentadesma butyracea seed butter or fat (PBSB) is a vegetable fat extracted from the seeds of the P. butyracea plant. The butter has potential use in the food, pharmaceutical, and cosmetic industries. The study investigated traditional PBSB processing methods in Ghana and evaluated their effects on yield, physicochemical parameters, and fatty acid composition. Four traditional extraction methods were identified and evaluated, and these methods are direct wet extraction of PBSB from a fresh mixture of the seed paste and water (DEW); wet extraction of PBSB from a 12-hour fermented mixture of the seed paste and water (FWO); direct wet extraction …