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Full-Text Articles in Food Science
Rapid Detection Of Listeria Monocytogenes, Wim Lippens
Rapid Detection Of Listeria Monocytogenes, Wim Lippens
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Listeria monocytogenes is a foodbome pathogen that can cause severe illness and even death. It is found in dairy and meat products. The focus is on rapid detection since conventional methods are time consuming (4-5 days). Pre-enrichment steps, as part of those methods, are time consuming. Our objective was to develop a detection system without a pre-enrichment step, giving a final result within 2 to 4 h.
In the concept of "the need for speed," a detection system with an antibody-based capture technique, followed by polymerase chain reaction (PCR), was developed. Glass beads coated with a Listeria polyclonal antibody were …
Tryptophan Catabolism In Brevibacterium Linens Bl2, Madhavi Ummadi
Tryptophan Catabolism In Brevibacterium Linens Bl2, Madhavi Ummadi
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Recent studies suggest aromatic amino acid catabolism by starter lactococci and flavor adjunct bacteria have a significant impact on off-flavor development during Cheddar cheese ripening. We hypothesized that a flavor adjunct bacterium, Brevibacterium linens BL2, produces off-flavor compounds from aromatic amino acid metabolism that will have a detrimental impact on cheese flavor.
The mechanism of tryptophan (Trp) catabolism in Brevibacterium linens BL2, was investigated in a chemically defined medium during incubation in laboratory conditions (no carbohydrate, pH 6.50, 220 rpm, 25°C) and cheese-like conditions (no carbohydrate, 4% NaCl, static incubation, l5°C). In laboratory conditions, metabolic studies and enzyme assays confirmed …