Entomology Commons^™

Effect Of Environmental Conditions On German Cockroach (Blattella Germanica) Behavior And Survival, Allison Lopina

Theses and Dissertations--Entomology

German cockroaches (Blattella germanica L.) remain one of the most challenging indoor pests to manage, due to resistance to many commonly used insecticides, and necessary contact/interaction with common management tactics. As such, it is critical to fully understand their aggregation, movement, and longevity, such that treatments can be optimally placed to result in maximal efficacy. The first part of this study explored the effects of multiple factors (harborage width, harborage materials, heat, resource-harborage distance) on initial site selection, and how environmental modifications and infestation density impact harborage site switching. The second half of the study aimed to explore the …

Limitations Of Reproductive Manipulation By Bacterial Endosymbionts In A Spider Host, Jordyn D. Proctor

Theses and Dissertations--Entomology

Maternally inherited bacterial endosymbionts are common in arthropods. Many of these bacteria manipulate host reproduction to favor the production of infected females, thereby increasing bacterial prevalence in the host population, even if cost is incurred to the host. However, there are also many factors that may inhibit symbionts’ ability to spread in host populations, including environmental constraints, symbiont-imposed costs on the host, interactions with co-infecting bacteria, or host biology. I explored two aspects of symbiont biology within a spider host, Mermessus fradeorum (Linyphiidae), which has varying combinations of up to five different bacterial endosymbionts in local populations: Rickettsiella, Tisiphia …

Impacts Of Livestock Dewormers On Dung Beetle Foraging Choices Across Spatial Scales, Madison L. Kluge

Theses and Dissertations--Entomology

Dung beetles provide ecosystem services in pasture systems but are vulnerable to chemical residues in livestock dung. Parasiticides containing macrocyclic lactones (MLs) are used to control parasites in livestock and are excreted unmetabolized in dung, exposing non-target organisms to these compounds. We used a multi-scale approach to examine whether ML residues alter dung beetle foraging ecology. At the field scale, we assessed dung beetle abundance and community composition using traps baited with dung from untreated cattle and cattle treated with ivermectin, moxidectin, or eprinomectin across eight farms and natural areas. At a smaller scale, we conducted behavioral assays to quantify …

Seasonal Population Dynamics And Interspecific Interactions Of Drosophila Suzukii, Eleanor Mccabe

Theses and Dissertations--Entomology

Invasive species are a growing problem, and it is important to investigate how invasives interact with novel ecosystems, which are characterized by distinct abiotic and biotic factors. In this dissertation, I investigate abiotic and biotic factors affecting two invasive pests in central Kentucky, Drosophila suzukii and Zaprionus indianus. In Kentucky, seasonality, and landscape features on population dynamics and fitness are unknown. I found that D. suzukii overwinters in Kentucky and that wild berry species improve winter survival. I used local weather data to identify meteorological variables that best predict D. suzukii populations. I tested the extent to which D. suzukii …

German Cockroach Allergen Mitigation, Olivia Katz

Theses and Dissertations--Entomology

German cockroaches (Blatella germanica L.) remain one of the most challenging indoor pests to manage, due in large part to their cryptic nature, rapid reproduction, and resistance to insecticides. Because of their close proximity to humans, they also pose several serious health threats, including the production and dissemination of several potent allergens linked to asthma development and exacerbation. Given the risks associated with cockroach allergens, we examined the longevity of two distinct cockroach allergens (Bla g 1 and Bla g 5) in the home environment and the efficacy of cleaners at removing these allergens. Our findings show that Bla …

Including Black Farmers, Excluding Pests: Perspectives On Participation In The Cooperative Extension Service, Briana Bazile

Theses and Dissertations--Entomology

In recent years, the U.S. Department of Agriculture has faced renewed scrutiny for documented discrimination against Black farmers, yet far less attention has focused on its century-old partner in public service delivery, the Cooperative Extension Service (CES). This gap is especially notable considering 1) federal parity mandates extant since 1972 in response to significant disparities between Black and White constituents and staff,2) public commitments to Diversity, Equity & Inclusion in 2020 and, 3) the contributions of Black leaders at the origins of CES, at Tuskegee University in the late nineteenth century. Using the Kentucky Cooperative Extension Service (KYCES) as a …

Causes And Consequences Of Reproductive Plasticity In The Seed Beetle, Callosobruchus Maculatus, Josiah P. Ritchey

Theses and Dissertations--Entomology

Competition for resources has direct effects on traits that can influence an individual’s reproductive success in competitive mating environments. When population density is high during development, competition for resources can reduce growth and body size at maturation, influencing resources available for reproduction. In this thesis, I investigate how the developmental environment affects plasticity in male reproductive traits, as well as the consequences of male reproductive plasticity on female reproductive behavior, in the seed beetle, Callosobruchus maculatus. My results show that increasing larval density causes male beetles to emerge small and transfer small ejaculates to females. However, small males transfer …

Dinotefuran Resistance In The German Cockroach (Blattella Germanica L.), Hannah F. Blevins

Theses and Dissertations--Entomology

German cockroaches (Blattella germanica L.) are one of the most prevalent and pervasive indoor pest species in the world. Infestations can lead to many negative impacts on humans, including sensitization to allergens, increase in asthmatic symptoms, mental health declines, and potentially, the spread of diseases. While insecticides remain the most effective and affordable option for German cockroach control, resistance to many commonly used insecticide active ingredients (AIs), such as pyrethroids, has been observed globally at high levels across different populations. Given such resistance to pyrethroids, inclusion of insecticides with alternative modes of action into insecticidal treatments could potentially improve cockroach …

Bed Bugs: Health Risks And Control, Simona Principato

Theses and Dissertations--Entomology

Bed bugs are blood-feeding hemipterans strongly associated with humans. Their detection in indoor environments, such as homes, hotels, trains, and public facilities, has increased gradually over the past 20 years. The exact cause of their resurgence is still unclear, but the increase of international travel and insecticide resistance are thought to have contributed to it.

Although bed bugs are not known to transmit diseases, the EPA, CDC, and USDA have classified them as a pest of significant public health concern, mainly due to the dermatitis that they can cause. Beyond the dermatological burden of the bites, other health implications often …

The Role Of Nutrition In Managing Honey Bee Viral Pathogen, Caroline Wylie Kane

Theses and Dissertations--Entomology

This study evaluated the impact of nutritional supplements on viral infections in honey bee colonies, focusing on Lake Sinai Virus 2 (LSV2), Deformed Wing Virus A (DWVA), and Deformed Wing Virus B (DWVB). I partnered with 32 hobbyist beekeepers across Kentucky, who volunteered 138 colonies for a month-long field study. Half of the colonies received weekly commercial supplements, while the other half served as controls. Viral titers were measured before and after the supplementation period using qPCR. Supplements did not significantly reduce viral titers for any of the three viruses, suggesting that short-term supplementation may not be effective in lowering …

Polymorphism And Plasticity In The Termite Caste System, Austin Merchant

Theses and Dissertations--Entomology

Developmental plasticity, manifested as a caste system, is integral to the success of eusocial insects because of the way in which it decouples the various tasks that a single, solitary organism would normally be responsible for, allowing individuals to specialize towards their given roles while keeping vulnerable members of the colony, such as those responsible for reproduction, safe from harm. Termites exhibit particularly complex caste systems that can be divided into three types of castes: workers perform manual labor, such as foraging and brood care; soldiers defend the nest; and reproductives lay eggs. Each of these castes, as well as …

An Rnai-Based Approach To Ips Calligraphus Management: A Promising Strategy For Forest Protection, Mary Wallace

Theses and Dissertations--Entomology

As climate change leads to more frequent and severe extreme weather, host tree stress is increasing the occurrence of large-scale bark beetle outbreaks. One such beetle, Ips calligraphus (Germar), a native bark beetle in the southeastern United States, has traditionally been considered a secondary pest of pine trees. Management has relied on silvicultural practices that enhance forest resilience, enabling host trees to effectively use natural defenses to deter beetle infestation. However, during periods of forest stress caused by storms, droughts, human disturbance, and other factors, beetle outbreaks can cause considerable ecological and economic damage.

Addressing worsening I. calligraphus outbreaks will …

Indoor Pests And Human Health: Characterizing Contaminants And Improving Mitigation, Johnalyn Gordon

Theses and Dissertations--Entomology

Much of the United States population spends the majority of their time indoors and within the home. Not only are indoor arthropod pests an unsightly nuisance in our homes, but they can have serious impacts on human health, especially with chronic exposure. In addition to negative health impacts, costs associated with these medical impacts can be tremendous. For these reasons, it is critical to develop comprehensive strategies for pest reduction and mitigation of associated health impacts. The work outlined in this dissertation represent examination of the distribution of a hypothesized bed bug allergen within the indoor environment, identification of a …

Addressing The Trade-Offs Of Pest Protective Row Covers For Integrated Pest Management In Vegetable Production, Kathleen Fiske

Theses and Dissertations--Entomology

The success of many integrated pest management (IPM) strategies is limited by trade-offs with economic, cultural, and environmental factors. While some IPM strategies reduce pests, they may harm pollinators, reduce the efficiency of weed or disease management, or may increase costs relative to revenue. Pest protective row covers, in the form of fine-mesh netting (referred to as mesotunnels), are an effective alternative to pesticide use, but they are not without their challenges. While row covers exclude insect pests and the pathogens they vector, it is unknown how well non-vectored diseases can be managed underneath the row cover. The exclusion of …

Practical Challenges In Delivering Dsrna For Woody Plant Protection, Zachary Bragg

Theses and Dissertations--Entomology

Unprecedented weather events associated with climate change, coupled with extensive anthropogenic stresses, have reduced the effectiveness of traditional forest pest management approaches, which can no longer keep pace with invasive species overwhelming naïve landscapes and native pests experiencing eruptive and expansive outbreaks. Double-stranded RNA-(dsRNA) mediated gene silencing, a type of RNA interference (RNAi), has been coopted for use as a biopesticide against a multitude of agricultural and horticultural pests and could serve as a powerful tool for woody plant protection. While effective initiation of gene silencing and subsequent mortality have been demonstrated in multiple tree pests, effective and efficient methods …

Consequences Of Abiotic And Anthropogenic Stressors In The Antarctic Midge, Belgica Antarctica, Jack J. Devlin

Theses and Dissertations--Entomology

The ability to tolerate stress is key to maintaining fitness in extreme environments such as the polar regions. Subject to a myriad of extreme year-round abiotic conditions, invertebrates inhabiting the terrestrial ecosystems of Antarctica provide an excellent system to investigate adaptations to stress. Understanding the response of these species to abiotic and novel stressors is crucial in a world with rapidly occurring climate change and increasing anthropogenic activity. In this dissertation, I investigated physiological and molecular responses to naturally occurring and human-derived stresses in the midge Belgica antarctica, which is Antarctica’s only endemic insect.

In my first research chapter, I …

Evaluation Of Ten Alternative Treatments For The Management Of Harlequin Bug (Murgantia Histrionica) On Brassica Crops, Sarah Clark

Theses and Dissertations--Entomology

This thesis investigates the efficacy of exclusion netting and selected biopesticides in controlling the common brassica pest harlequin bug (Murgantia histrionica); it assesses the phytotoxic effects caused by selected essential oil products; and it evaluates whether selected treatments caused mortality in common beneficial insects. This is one of the first studies to investigate the potential pest controlling properties of commercially produced bokashi fermented composting products as well as their effect on natural enemies.

During both the first and second year of the study, none of the commercially produced essential oil products or bokashi products were effective in controlling …

Weed Management Impacts On Wild Bees In Agroecosystems, Amanda Denny

Theses and Dissertations--Entomology

In agricultural landscapes, bee communities are shaped by factors including the plant community, land management, and pesticides. These factors are critical in the late winter and early spring (LWES) when floral resources are scarce and many bee species are becoming active. In this dissertation, I characterize the flowering weed community of Central Kentucky agroecosystems in the LWES, examine the response of wild bee communities to the total cover and diversity of these flowering plants, and assess the risks of glyphosate exposure on developing ground nesting bees. I found that the flowering plant community was similar across land cover types and …

Rnai-Mediated Gene Silencing In The Exotic Redbay Ambrosia Beetle, Xyleborus Glabratus, And Insect-Fungal Interactions Within The Laurel Wilt Complex, Morgan Christine Knutsen

Theses and Dissertations--Entomology

Laurel wilt disease (LWD) is a lethal vascular disease impacting lauraceous hosts caused by Harringtonia lauricola, the fungal symbiont of the redbay ambrosia beetle (Xyleborus glabratus Eichoff) (RAB) (Coleoptera: Curculionidae). LWD has caused tree mortality throughout the southeastern United States and is continuing to spread into new regions. Current management methods have not been successful in preventing spread, warranting investigation into innovative techniques including RNA interference (RNAi).

Elongation factor-1 alpha (ef1a) and actin (act) were established as stably expressed reference genes after exposing beetles to different photoperiod, temperature, and dsRNA exposure. After RAB oral …

Linking Previous Experiences To Behavior And Health In The Honey Bee (Apis Mellifera), Rebecca R. Westwick

Theses and Dissertations--Entomology

An organism’s ability to respond to changing conditions can be vital to its success. Indeed, plasticity is a common feature of living organisms. Much of the research in this area, though, has focused on effects caused by environmental conditions. What has received relatively less attention is how social experiences and broader features of an organism’s social environment can lead to long-lasting changes in health and behavior. This knowledge gap exists despite the well-documented existence of health and behavioral effects after social interactions in certain taxa such as humans.

Social insects such as honey bees provide an excellent opportunity to better …

Epigenetic Regulation Of Insect Development, Yaoyu Jiao

Theses and Dissertations--Entomology

Insects undergo a series of distinct life stages such as egg, larva and pupa to develop into mature adults capable of reproduction throughout their life cycle. In the larval stage, insects experience the fastest growth with voracious feeding and multiple cuticle shedding resulting in hundreds-of-fold increasing of size. During metamorphosis, insects stop increasing body size but go through dramatic tissue transformation, developing wings and other adult features. The characteristic mode of insect development, especially the metamorphosis, makes insects the most successful group of animals on the earth. However, the epigenetic mechanisms behind insect development remain elusive. Studying the epigenetic regulation …

Foraging Behavior In The Eastern Subterranean Termite, Reticulitermes Flavipes (Kollar) (Isoptera: Rhinotermitidae), Anugerah Fajar

Theses and Dissertations--Entomology

Foraging is essential for survival, but it can be unpredictably dangerous due to organismal interactions like competition and predation to various environmental factors. Animals have come up with strategies to safeguard foraging. Social insects, with their elaborate division of labor, have developed sophisticated systems of foraging task allocation that allow them to capitalize resources beyond their competitors. In this thesis, I delve into the subjects of foraging behavior, allocation strategies, and the genetic basis of foraging in the eastern subterranean termite, Reticulitermes flavipes, the most widely distributed termite species in North America and one of the most economically important …

Understanding The Effects Of Age, Environmental Conditions, And Placement On Cockroach Gel Bait Performance, Isabelle Lucero

Theses and Dissertations--Entomology

Cockroach baits are one of the most effective tools used for German cockroach (Blattella germanica L.) control. This is due in part to the number of cockroach baits on the market with various matrix compositions and active ingredients, aiding in the control of resistant (physiologically and behaviorally) cockroach populations through bait rotation. However, it remains unclear how cockroach gel baits perform over time and under different environmental conditions. Therefore, we aged six cockroach gel baits for varying times (24 hours, one month, three months, six months) and at three relative humidities (15%, 40%, and 80%), and tested their performance …

Mechanisms Of Rnai In Insects, Jin Mo Koo

Theses and Dissertations--Entomology

RNA interference (RNAi) mechanism is found in many eukaryotes and animal cells, with its endogenous functions as defense against transposable elements and viruses, as well as post transcriptional regulation of gene expression. Since its first discovery, RNAi has contributed enormously as a reverse genetic tool for functional genomic studies. In application, RNAi is used in therapeutics, as well as agricultural crop and livestock protection. Yet, for the wider application of RNAi, improvement of its potency and delivery technologies is needed. Deeper mechanistic understanding of every step of RNAi, from cellular uptake of RNAi trigger molecule to targeted mRNA degradation is …

Costs And Benefits Of Integrating Poultry Into Cover Crop - Vegetable Rotations, Viktor Halmos

Theses and Dissertations--Entomology

Integrated livestock systems were once common leading up to the 20th century where livestock generated manure that was then used to fertilizer crop production in a closed- or semi-closed system. However, with the industrialization of agriculture in the mid to late 20th century, specialization drove simplified systems that segregated livestock from cropping systems. This segregation led to nutrient surpluses occurring in livestock operations, and nutrient demands in cropping systems creating a plethora of environmental issues. With the increased awareness of environmental issues, a renewed interest in integration has driven producers and researchers to experiment with integration again. For research, the …

Managing Death In Termites, Jizhe Shi

Theses and Dissertations--Entomology

Death of individuals from the same species represents potential risks from disease, predation or competition for animals. Diverse responses associated with death have evolved ranging from simply avoiding or being attracted to the corpses in solitary animals to complicated undertaking behavioral repertoire in eusocial insects. A systematic review in chapter 1 suggested cannibalism is an ancestral and widespread death-related behavior in all non-human animals. Termites are suggested to switch their undertaking behavioral responses from cannibalism to burial based on interactions between chemicals associated with death to balance risks and benefits associated with decomposition. In eusocial animals like termites with caste …

Surveillance For Ticks And Tick-Borne Pathogens In Kentucky, Anna Rosalee Pasternak

Theses and Dissertations--Entomology

Tick-borne diseases are an emerging threat to human and animal health. In Kentucky, tick-borne disease surveillance has identified rising incidences of spotted fever rickettsiosis, ehrlichiosis, and Lyme disease. Since these diseases occur through the bites of infected ticks, effective prevention efforts are reliant upon knowing where the risk of exposure to tick bites exists. Historical data on tick distribution in Kentucky is variable, with very little reported on a statewide scale, leaving vector control workers, public health personnel, physicians, veterinarians, and others to rely on outdated, intermittent, or out-of-state information. In my dissertation, I surveyed ticks and select tick-borne pathogens …

Landscape Influences And Preventive Management For Drosophila Suzukii In Small Fruit Crops, Ryan William Kuesel

Theses and Dissertations--Entomology

Small fruit production has been greatly challenged by the repeated invasion of non-native arthropod pests, impacting economic feasibility, and increasing the need for insecticides. Spotted wing drosophila (Drosophila suzukii (Matsumura)) is the most recent invasive pest of small fruits. It causes damage by ovipositing beneath the skin of ripening fruits. Its wide-spread distribution and development of resistance to insecticides has resulted in the need for improved alternative management strategies for small fruit growers.

My surveys of D. suzukii at thirteen blackberry (Rubus fruticosus L.) and raspberry (Rubus idaeus L.) fields found that while D. suzukii was present …

Ecological Risk Assessment Of Transgenic Conditional Lethality Systems For Genetic Biocontrol Strategies, Fernan Rodrigo Perez-Galvez

Theses and Dissertations--Entomology

Genetic strategies of insect pest control are receiving increased interest due to recent advances in genetic engineering. By introducing alleles that make males functionally sterile, genetically modified (GM) insects can be used in area-wide pest management programs similar to the Sterile Insect Technique (SIT). Genetic control strategies carry several potential benefits, including improved efficacy and specificity, lower operation costs, and reduced dependence on chemical insecticides, but their use is still controversial despite extensive technical documentation indicating their innocuity to humans and the environment. This hesitation is likely due to the novel nature of the approach.

While the first field applications …

Management Of The Brown Marmorated Stink Bug (Halyomorpha Halys) (Hemiptera:Pentatomidae) On Small Acreage And Organic Vegetable Systems, Lauren Fann

Theses and Dissertations--Entomology

Invasive species can be very disruptive to established integrated pest management programs. They can cause increases in broad-spectrum insecticide applications with variable effectiveness to mitigate damage. However, not all introduced species develop invasive characteristics; often, many introduced species go undetected. It is not until a species begins to cause economic damage to commodities essential to humans that it becomes classified as an invasive pest. Many challenges arise with the management of invasive pests due to lack of understanding of their biology, behavior, and absence of natural enemies in their introduced range. This often results in poor management and population outbreaks …