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Articles 1 - 30 of 77
Full-Text Articles in Ecology and Evolutionary Biology
Small Mammal Species Richness And Relative Abundance Across The Elevational Gradient And Microhabitats Of Pine Mountain, Eastern Kentucky, Sarah Elizabeth Baker
Small Mammal Species Richness And Relative Abundance Across The Elevational Gradient And Microhabitats Of Pine Mountain, Eastern Kentucky, Sarah Elizabeth Baker
Online Theses and Dissertations
Identifying microhabitat conditions influencing species abundance is critical to understanding distributions of species. Pine Mountain has forest types uncommon in Kentucky, but systematic small mammal surveys are dated. My objectives were: investigate variation in small mammal occurrence on Pine Mountain, and update small mammal records in the Eastern Kentucky University (EKU) mammal collection. I deployed pitfall traps, open from May - November 2021, at four state nature preserves (SNPs) on Pine Mountain. I measured microhabitat using 1 m2 quadrats at traps, then used redundancy analysis and generalized linear mixed (GLM) models to determine which conditions best explained variation in occurrence …
Phenotypic Plasticity Of Geographically Central And Peripheral Populations Of Jefferson Salamanders (Ambystoma Jeffersonianum) In Response To Simulated Climate Change, Aaron Devine
Online Theses and Dissertations
Climate change will elicit various species responses as it causes habitats to change. Species with limited dispersal must respond to these changes “in place”, yet some biogeographical hypotheses indicate isolated and less abundant populations experience reduced gene flow and genetic diversity that may limit phenotypic plasticity, contrasting with evidence suggesting more variable habitats select for increased plasticity in peripheral populations. To gain insight into associations between range position and intensity of plasticity, mesocosm experiments were done to assess plasticity in larval growth, size at, and time to, metamorphosis, and survivorship in larval Ambystoma jeffersonianum salamanders from geographically core and peripheral …
Body Size And Habitat Shading Influence The Consumptive And Non-Consumptive Effects Of Wood Frog (Lithobates Sylvaticus) Tadpoles On Aquatic Invertebrate Communities, Brady Patrick Parlato
Body Size And Habitat Shading Influence The Consumptive And Non-Consumptive Effects Of Wood Frog (Lithobates Sylvaticus) Tadpoles On Aquatic Invertebrate Communities, Brady Patrick Parlato
Online Theses and Dissertations
Consumptive (direct) effects of predation have been well-studied in most aquatic systems, but non-consumptive (indirect) effects on prey have only recently received limited attention in some groups. As aquatic consumers, tadpoles have traditionally been considered strict herbivores, leaving their predatory impacts via consumptive and non-consumptive interactions on aquatic invertebrates largely unexplored. The objectives of this study were to quantify omnivory among wood frog (Lithobates sylvaticus) tadpoles, determine their consumptive and non-consumptive effects on invertebrate communities, and investigate whether such effects are mediated by habitat shading and tadpole body size. Lithobates sylvaticus tadpoles and egg masses were randomly sampled from 11 …
Genetic And Morphological Assessment Of Pigtoe Mussels (Unionidae: Pleurobemini: Fusconaia/Pleurobema Spp.) In Ozark Drainages, Logan Tyler Phelps
Genetic And Morphological Assessment Of Pigtoe Mussels (Unionidae: Pleurobemini: Fusconaia/Pleurobema Spp.) In Ozark Drainages, Logan Tyler Phelps
Online Theses and Dissertations
North America is home to nearly 300 species of native freshwater mussels. Many species within this group are in need of conservation efforts and for these efforts to be effective, species delimitations must be as accurate as possible. Intraspecific variation and interspecific convergence are sources of confusion within morphology-based taxonomy, particularly within the Pleurobemini. Molecular phylogenetic work has revealed multiple problems within currently accepted Pleurobemini classifications. Specifically, Fusconaia has been shown to harbor cryptic diversity within drainages of the Ozarks. Further, Pleurobema rubrum and P. sintoxia have been shown to be possible conspecifics despite have differing shell morphologies. This study …
Comparison Of Spawning Habitat And Nest Density Between Buck Darter (Etheostoma Nebra) And Striped Darter (Etheostoma Virgatum) Populations In The Cumberland River Drainage, Kentucky, Jacob Charles Murphy
Comparison Of Spawning Habitat And Nest Density Between Buck Darter (Etheostoma Nebra) And Striped Darter (Etheostoma Virgatum) Populations In The Cumberland River Drainage, Kentucky, Jacob Charles Murphy
Online Theses and Dissertations
The Buck Darter (Etheostoma nebra) is an imperiled, small-stream species in the Cumberland River drainage in southern Kentucky. It was originally thought to be a population of the Striped Darter (E. virgatum) species and was prevalent throughout the Buck Creek system. It was separated from the Striped Darter and deemed a separate species in 2015; however, the population has declined dramatically. Presently, the species is found in three streams of the Flat Lick Creek system, two originally established populations in Big Spring Branch and Stewart Branch, and one newly introduced population, established in 2018, in an unnamed tributary. The decline …
Litter Breakdown And Benthic Invertebrate Detritivores From A Hydrologically Restored Stream, Jacob Becraft
Litter Breakdown And Benthic Invertebrate Detritivores From A Hydrologically Restored Stream, Jacob Becraft
Online Theses and Dissertations
The breakdown of coarse forms of detritus into finer forms is an ecosystem function of forested headwater streams. Many factors influence the rate of litter decay, but there is a lack of research on how stream restorations, that aim to restore hydrologic functions, influence litter breakdown. This study compared litter processing rates and macroinvertebrate detritivore assemblages between a hydrologically restored stream (Slabcamp Creek) and an un-restored control stream (White Pine Branch) in the Daniel Boone National Forest in Rowan Co., KY. Red maple (Acer rubrum) leaves were placed in mesh bags and deployed in riffles and pools at the restored …
Assessment Of Indiana Bat Reproductive Condition, Recapture Trends, And Temperatures Of Artificial Roosts In Kentucky, Michaela L. Rogers
Assessment Of Indiana Bat Reproductive Condition, Recapture Trends, And Temperatures Of Artificial Roosts In Kentucky, Michaela L. Rogers
Online Theses and Dissertations
Recovery of bat species impacted by white-nose syndrome (WNS) will necessarily require population growth. I assessed reproductive capability of the endangered Indiana bat (Myotis sodalis) in Kentucky, where WNS was first detected in hibernacula in April 2011. Due to loss of fat reserves associated with WNS infection, coupled with the energetic expenditures associated with pregnancy, I hypothesized mass of reproductively-active females captured during the maternity season would decrease across my data collection period (2017-2019). Further, I predicted that reproductive rates in the study population would be lower than historic rates for Kentucky. Mist net surveys around artificial maternity roosts at …
Bat Boxes As Mitigation Tools: Factors Impacting Microclimate And Myotis Sodalis Roost Selection, Reed Crawford
Bat Boxes As Mitigation Tools: Factors Impacting Microclimate And Myotis Sodalis Roost Selection, Reed Crawford
Online Theses and Dissertations
Environmental degradation has led to declines in available natural roosting habitat for bats. To mitigate this loss, practitioners often deploy artificial roosts (e.g., bat boxes). There are no established species-specific practices for deployment strategy and roost design selection, but occupancy rates are known to vary across species and roost microclimates can be harmful to bats. Providing bats with thermally beneficial roosts during summer could enhance overwinter survival of WNS-affected species. To further our understanding of roost preference and microclimate, we deployed 40 rocket box roosts of 5 designs at field sites in Indiana and Kentucky. Roosts were deployed in clusters …
The Importance Of Surface Mining As A Landscape Variable In Shaping Wild Bee Communities In Central Appalachian Forests, Amanda Sheryl Dunaway
The Importance Of Surface Mining As A Landscape Variable In Shaping Wild Bee Communities In Central Appalachian Forests, Amanda Sheryl Dunaway
Online Theses and Dissertations
Anthropogenic disturbance has led to widespread losses in biodiversity. Native bees (Hymenoptera, Apoidea) provide essential pollination services and have been declining in abundance dramatically in recent years. One potential cause of these declines is anthropogenic disturbance. Eleven sites were established in Southeastern Kentucky, U.S.A. where surface mining for coal is common. Data was analyzed using generalized linear mixed-effect models to detect the most important landscape scale variables, including mining, in shaping bee communities. Bee species richness was significantly lower on sites that contained surface mines, but abundance was similar between mined and unmined sites. The proportion of each nesting group …
Possible Role Of Breeding Phenology, Brood Size, And Male Parental Care On The Number Of Nesting Attempts By Female Eastern Bluebirds, A Facultatively Multibrooded Species, Michael Daniel Patton
Possible Role Of Breeding Phenology, Brood Size, And Male Parental Care On The Number Of Nesting Attempts By Female Eastern Bluebirds, A Facultatively Multibrooded Species, Michael Daniel Patton
Online Theses and Dissertations
To maximize reproductive output, several species of songbirds attempt to raise two or more broods in a single breeding season. The results of previous studies have revealed much variation among species in the factors that influence the likelihood of female songbirds attempting to raise multiple broods during a breeding season. As such, additional studies are needed to better understand the roles of early breeding, brood sizes, and, especially, male parental care in determining the likelihood of having multiple broods. My objective, therefore, was to examine the possible effects of breeding initiation date, brood size, and male provisioning behavior on the …
Abundance Of Songbirds In Eastern Hemlock Stands Following Chemical Treatment For Hemlock Woolly Adelgid, Natalie Sweeting
Abundance Of Songbirds In Eastern Hemlock Stands Following Chemical Treatment For Hemlock Woolly Adelgid, Natalie Sweeting
Online Theses and Dissertations
In the 1960’s the invasive Hemlock Woolly Adelgid (Adelges tsugae [hereafter, HWA]) began to spread west across the hemlock stands of the Eastern U.S. killing a significant number of Eastern Hemlock (Tsuga canadensis). While chemical treatments, primarily with the active ingredient imidacloprid, have been implemented, their effects on hemlock dependent avian species are largely unknown. A 2009 study, which took place as HWA was beginning to invade Kentucky, identified six indicator species that were positively and negatively correlated with eastern hemlock stands throughout the Appalachian Mountain region of Kentucky. Our study repeated bird and vegetation surveys at the same 65 …
Bee Abundance Along A Tropical Montane Elevational Gradient And Implications For Crop Pollination Services, Kristin M. Conrad
Bee Abundance Along A Tropical Montane Elevational Gradient And Implications For Crop Pollination Services, Kristin M. Conrad
Online Theses and Dissertations
Tropical forests are among the biologically richest ecosystems on Earth, but how most organisms in these forests will respond to a warming climate remains uncertain. Insects are expected to be highly responsive to climate change due to their short life cycles that are strongly influenced by temperature. Plants depend on pollinators to set seed and reproduce, and many animal populations rely on the resources provided by flowering plants. There is an urgent need to document elevational distributions and thermal specialization for tropical bee species to understand how these important pollinators may respond to warming temperatures. My four-year study (2016-2019) aims …
Impacts Of Driving Factors On Annual Cicada Emergence In Relation To The Seasonal Abundance Of Eastern Copperheads (Agkistrodon Contortrix), Jesse Christian Sockman
Impacts Of Driving Factors On Annual Cicada Emergence In Relation To The Seasonal Abundance Of Eastern Copperheads (Agkistrodon Contortrix), Jesse Christian Sockman
Online Theses and Dissertations
For many species, life history events are cyclical and often correspond to specific environmental conditions. As a result of environmental variability, the optimal conditions that regulate the range and activity of highly regulated cyclical organisms, like cicadas, are subject to temporal change. Variability in cicada emergence and abundance has been shown to impact the species that rely on the rapid influx of nutrients. One such predator is the Eastern Copperhead (Agkistrodon contortrix) that has been noted feeding on emerging cicadas throughout their range. In eastern Kentucky, observations of copperheads congregating to feed on cicadas has been documented in recreational areas. …
Ecosystem Functional Consequences Of Top Predator Mortality Due To The Invasive Shrub, Lonicera Maackii, Josey Lee-Anne Berta
Ecosystem Functional Consequences Of Top Predator Mortality Due To The Invasive Shrub, Lonicera Maackii, Josey Lee-Anne Berta
Online Theses and Dissertations
Lonicera maackii (Amur Honeysuckle) is an invasive woody plant species that is present across the United States. Previous studies have assessed the biotic effects of honeysuckle, as well as abiotic effects such as changes in soil chemistry, ground level light, and forest floor temperature. Although directs effects of L. maackii on native terrestrial plant communities are well studied, little is known about its indirect effects, especially in aquatic ecosystems. Based on limited prior studies, I predicted addition of L. maackii leaves to aquatic systems would increase mortality of a top amphibian predator due to the release of phenolic compounds that …
Habitat Use And Nightly Activity Patterns Of Bats On A Military Landscape In Western Kentucky, Carson E. Mcnamara
Habitat Use And Nightly Activity Patterns Of Bats On A Military Landscape In Western Kentucky, Carson E. Mcnamara
Online Theses and Dissertations
Bat populations of eastern North America continue to decline due to the cumulative effects of White-nose Syndrome, habitat loss, and anthropogenic disturbances across the landscape. Unique stressors exist on military installations, such as noise created during training activities. Given the scarcity of data that exists for these widespread ownerships, I created predictive models for nightly bat activity related to local habitat, landscape, and military use parameters. Bat activity was assessed during the summers of 2016 and 2017 using full-spectrum acoustic detectors across the Wendell H. Ford Regional Training Center (WHFRTC), a ca. 4200 ha military landscape in the Interior Rivers …
Ecosystem Functional Consequences Of Body Size Variation In An Apex Predator (Ambystoma Jeffersonianum), David Samuel Smith
Ecosystem Functional Consequences Of Body Size Variation In An Apex Predator (Ambystoma Jeffersonianum), David Samuel Smith
Online Theses and Dissertations
Biodiversity is often emphasized at the species level where each species is assigned a mean functional trait value. However, populations within a species, and individuals within a population, often exhibit considerable intraspecific functional variation. Therefore, instead of focusing on species’ mean trait values, we must incorporate intraspecific variation when considering species’ ecological roles and conservation values. The primary objective of this study was to determine the effects of variation in body size (a functional trait in many aquatic taxa) in an apex predator on ecosystem functioning. We sought to characterize trophic cascades initiated by larval populations of Ambystoma jeffersonianum that …
Vocalizations Of Adult American Kestrels (Falco Sparverius): Effect Of Breeding Stage, Sex, And Context On Call Use And Characteristics, Mark Winland
Online Theses and Dissertations
American Kestrels (Falco sparverius) are small falcons with a vocal repertoire known to consist of three different vocalizations: whine, chitter, and klee calls. However, the characteristics and contextual use of these calls have not been quantified. To determine the characteristics of these calls and better understand possible functions, I conducted a combined observational and experimental study of American Kestrels in Madison County, Kentucky, from February to July 2013. I observed kestrels and recorded all vocalizations uttered by males and females during different breeding stages and different behavioral contexts. In addition, I conducted playback experiments using the whine, chitter, and klee …
Population Demographics And Active Foraging Behavior Of Copperheads (Agkistrodon Contortrix) At A Recreational Site In Eastern Kentucky, Jocelyn Hendricks
Population Demographics And Active Foraging Behavior Of Copperheads (Agkistrodon Contortrix) At A Recreational Site In Eastern Kentucky, Jocelyn Hendricks
Online Theses and Dissertations
Recreational areas represent a significant source of human-wildlife interaction, which can have an especially negative effect on organisms, like snakes, that are generally perceived as dangerous by the public. Considering the projected increase in recreation rates in the United States, the threat to this already vulnerable group of organisms deserves attention. While habitat factors associated with recreational areas—fragmentation, decreased canopy cover, altered vegetation composition—have been shown to affect snake thermoregulatory behavior and abundance, detailed studies of wild snake behavior are uncommon due to their cryptic nature. The goal of this study was to reduce human-wildlife conflict at a recreational site …
Perceived Predation Risk And The Responses Of Adult And Nestling Tree Swallows (Tachycineta Bicolor), Natalia May Maass
Perceived Predation Risk And The Responses Of Adult And Nestling Tree Swallows (Tachycineta Bicolor), Natalia May Maass
Online Theses and Dissertations
Some species of birds have alarm calls that are functionally referential and provide their nestlings with information about the level of threat predators may pose. Although several investigators have examined the responses of nestlings of species with open-cup nests to the anti-predator calls of adults, few have conducted such studies with cavity-nesting species of birds. Therefore, the objectives of my study were to examine the vocal responses of cavity-nesting adult Tree Swallows (Tachycineta bicolor) to different nest predators, some able and others unable to enter nest cavities, and to examine the responses of nestlings to those vocalizations. My study was …
The Use Of Environmental Dna For The Detection Of Palaemonias Ganteri (Hay, 1901), A Federally Endangered Cave Species, Andrew Joseph Stump
The Use Of Environmental Dna For The Detection Of Palaemonias Ganteri (Hay, 1901), A Federally Endangered Cave Species, Andrew Joseph Stump
Online Theses and Dissertations
Kentucky’s limestone caves and karst water systems are an ecologically important part of its natural heritage and home to many unique subterranean species. In addition to being geologically interesting, it is also home to one, of only three, extant North American atyids: the federally endangered Kentucky Cave Shrimp (Palaemonias ganteri, Hay 1901). However, access for monitoring and management objectives involving this species and other cave inhabitants can be difficult, highly contingent upon environmental conditions, and time consuming with low yields using traditional techniques. Advancements in metabarcoding and Next Generation Sequencing (NGS) technologies provide tools that may allow researchers and managers …
The Vascular Flora Of The Red River Gorge In Powell, Menifee, And Wolfe Counties Kentucky, Thomas Steele Mcfadden
The Vascular Flora Of The Red River Gorge In Powell, Menifee, And Wolfe Counties Kentucky, Thomas Steele Mcfadden
Online Theses and Dissertations
The Red River Gorge (RRG) is a 26,283 ha natural area within Menifee, Powell, and Wolfe counties of Kentucky. This area is in the Cumberland Plateau Physiographic Section which is a southern subsection of the Appalachian Plateau Physiographic Province. It is a mecca of outdoor recreation, hosting thousands of visitors each year, while also being home to a unique flora worthy of continued protection. A vascular flora was conducted between the fall of 2016 and the summer of 2018. Additional vouchers donated by Dr. Ross C. Clark and the Stanton Ranger District office, as well as vouchers deposited at regional …
Population Size And Habitat Association Of Etheostoma Nebra, The Buck Darter, Using Abundance Modeling In The Buck Creek System, Cumberland River Drainage, Kentucky, Harold Davidson Black
Population Size And Habitat Association Of Etheostoma Nebra, The Buck Darter, Using Abundance Modeling In The Buck Creek System, Cumberland River Drainage, Kentucky, Harold Davidson Black
Online Theses and Dissertations
Etheostoma nebra (Buck Darter) is a recently described fish species confined to the Buck Creek system, Cumberland River drainage, Kentucky. A 2010-2012 survey of Buck Creek by Kentucky Department of Fish and Wildlife Resources personnel observed E. nebra at 2 of 47 historical sites. Within the entire system, individuals were found only in Flat Lick Creek around the confluence of two spring-fed tributaries, Big Spring Branch and Stewart Branch. The objectives of this study were to: (1) determine population size, demographics, and habitat association of E. nebra in Big Spring Branch and Stewart Branch, (2) evaluate habitat conditions both within …
Development Of An Avian Index Of Biological Integrity For Kentucky Wetlands, Kaitlyn Kelly
Development Of An Avian Index Of Biological Integrity For Kentucky Wetlands, Kaitlyn Kelly
Online Theses and Dissertations
Bird communities are frequently used as bioindicators to assess environmental conditions, including in wetland habitats. I developed an avian index of biological integrity (IBI) for wetlands of Kentucky as an intensive assessment method to supplement an existing rapid assessment method used in regulatory programs. Birds are useful indicators because they are sensitive to environmental changes, abundant in various landscapes, occupy higher trophic levels, and can be sampled in a cost-effective manner. Breeding bird point count data from 103 sites were used to calculate a set of 49 avian community metrics. Avian metrics were tested for correlation with independent landscape, hydrology …
Variation In Mass And Wing Loading Of Nestling American Kestrels: Possible Effects Of Nestling Behavior And Adult Provisioning Behavior, Doug Mitchell
Variation In Mass And Wing Loading Of Nestling American Kestrels: Possible Effects Of Nestling Behavior And Adult Provisioning Behavior, Doug Mitchell
Online Theses and Dissertations
Among birds, the rapid growth rates of altricial young help reduce mortality by reducing the amount of time spent in the nest. However, in species where a high degree of maneuverability and speed is required (i.e. aerial insectivores), it is important that nestlings not gain excess weight. Nestlings in some species must attain an efficient wing loading just prior to fledging to facilitate mobility for hunting and evading predators. My objective was to examine the mass of nestling American Kestrels (Falco sparverius) during the mid- to late nestling period and specifically to determine the possible effects of attaching small lead …
Amphibian And Plant Communities Of Natural And Constructed Upland-Embedded Wetlands In The Daniel Boone National Forest, Rachel Fedders
Amphibian And Plant Communities Of Natural And Constructed Upland-Embedded Wetlands In The Daniel Boone National Forest, Rachel Fedders
Online Theses and Dissertations
Wetlands fulfill many vital ecological functions, including providing habitat for amphibians and plants. Some wetlands, known as upland-embedded wetlands (UEWs), are depressional wetlands surrounded completely by upland habitat. This wetland type has been constructed in many areas for conservation and mitigation purposes, but constructed UEWs often do not function equivalently to natural wetlands, and often have different physical and chemical characteristics. In the Daniel Boone National Forest (DBNF), numerous UEWs have been constructed on ridge-tops to benefit game and bat species. Previous studies have shown that many of these constructed wetlands have permanent hydroperiods and different amphibian communities than co-occurring …
Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers
Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers
Online Theses and Dissertations
Freshwater mussels have become some of the most imperiled species in North America and widespread populations have succumbed to pollution and many other anthropogenic-related factors. With molecular techniques evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total DNA yield (ng/mg) from mussel tissue. The hinge ligaments of freshwater mussels ranging in collection date (1984-2015) were used as the source of genetic …
Comparison Of Cytochrome B Variation And Morphological Features Of Isolated Banded Pygmy Sunfish (Elassoma Zonatum) Populations In The Lower Green River And Coastal Plain Systems In Western Kentucky, David Cravens
Online Theses and Dissertations
The banded pygmy sunfish Elassoma zonatum is a wide-ranging species found throughout the Southeastern Coastal Plain of North America. In Kentucky, the populations are found above and below the inner boundary of the Coastal Plain. Due to their geographical separation comparisons in body size and morphological features along with genetic comparisons using mitochondrial cytochrome-b (Cyt-b) were conducted to test relationships between populations. Two sites, Cypress Creek in the Lower Green River system and Rose Creek in the Tradewater River drainage, were sampled from above the inner boundary and two additional sites, Bayou de Chien and Running Slough in the Mississippi …
Habitat Use By Wintering Passerines In Fire-Managed Forests Along The U.S. Gulf Coast, Justin Michael Michaud
Habitat Use By Wintering Passerines In Fire-Managed Forests Along The U.S. Gulf Coast, Justin Michael Michaud
Online Theses and Dissertations
Coastal habitats are being impacted by land development, fragmentation, and disturbance related to climate change. The remaining natural areas need to use planned management that may, in some cases, include the use of prescribed fire to maintain habitat quality. Numerous species of passerines, including some with declining populations, use the Gulf Coast as a wintering area, and some depend on habitats managed by fire. To provide information for land managers, I studied the winter bird community at Naval Live Oaks in Gulf Islands National Seashore with two primary objectives: (1) to describe the distribution, abundance, and diversity of the non-breeding …
Natural Environmental Gradients Predict The Microhabitat Use, Fine-Scale Distribution, And Abundance Of Three Woodland Salamanders In An Old-Growth Forest, Joseph Alex Baecher
Natural Environmental Gradients Predict The Microhabitat Use, Fine-Scale Distribution, And Abundance Of Three Woodland Salamanders In An Old-Growth Forest, Joseph Alex Baecher
Online Theses and Dissertations
Woodland salamanders (Plethodonidae: Plethodon)－a group of sensitive, direct developing, lungless amphibians－are particularly responsive to gradients in environmental conditions. Because of their functional dominance in terrestrial ecosystems, woodland salamanders are responsible for the transformation of nutrients and translocation of energy between highly desperate levels of trophic organization (detrital food webs and high‐order predators). However, the spatial extent of woodland salamanders' role in the ecosystem is likely contingent upon the distribution of their biomass throughout the forest. Therefore, a better understanding of woodland salamander spatial population dynamics is needed to further understand their role in terrestrial ecosystems. The objectives of this study …
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Online Theses and Dissertations
DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high throughput sequencing and the proliferation of sequence data in public repositories, insights to the biodiversity of communities at the molecular level have been possible. Thus, this study compared commonly used DNA capture (water precipitation and filtration) and extraction (MoBio's PowerWater, Qiagen's DNeasy Blood and Tissue Kit, and a CTAB protocol) methods in their …