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Articles 1 - 5 of 5
Full-Text Articles in Ecology and Evolutionary Biology
Environmental Influences On Expression Of Virulence- And Survival-Associated Genes And Epigenetic Modifications Of Dna In Vibrio Vulnificus, James W. Conrad
Environmental Influences On Expression Of Virulence- And Survival-Associated Genes And Epigenetic Modifications Of Dna In Vibrio Vulnificus, James W. Conrad
USF Tampa Graduate Theses and Dissertations
Vibrio vulnificus is an autochthonous inhabitant of warm, brackish coastal waters and is an opportunistic pathogen that causes the highest mortality of all seafood-related illnesses (1). These fatal infections are generally caused by the clinically-associated vcgC/16S rRNA type B genotypes within the biotype I group (2–5). However, the reasons for the heightened infectiousness of the clinically-associated strains over environmentally-associated ones remains elusive, as no unique clinically-associated virulence genes have been identified through genomic sequencing or other strategies. DNA methylation may contribute to regulation of virulence by affecting gene transcription, and was investigated in the highly virulent V. vulnificus strain CMCP6. …
Microbial Community Structures In Three Bahamian Blue Holes, Meghan J. Gordon
Microbial Community Structures In Three Bahamian Blue Holes, Meghan J. Gordon
USF Tampa Graduate Theses and Dissertations
This study used 16S rDNA metagenomics and water chemistry to conduct an examination of microbial community dynamics and biogeochemistry in three physically adjacent, sunlit blue holes with variable hydrologic regimes on San Salvador Island, Bahamas. Church and Watling’s Blue Holes are holomictic with relatively clear waters, while Inkwell Blue Hole hosts density stratification and waters stained brown with tannins. Based on water color and clarity and physicochemical profiles, I hypothesized Church and Watling’s Blue Holes would be dominated by oxigenic photoautotrophs, and that the bottom layer of Inkwell would be characterized by euxinic (anoxic and sulfidic) conditions and host primarily …
The Ecology And Conservation Of An Urban Karst Subterranean Estuary, Robert J. Scharping
The Ecology And Conservation Of An Urban Karst Subterranean Estuary, Robert J. Scharping
USF Tampa Graduate Theses and Dissertations
Sulphur Springs is an artesian spring and part of the limestone karst topography of metropolitan Tampa, Florida, USA. Underlying this spring is an extensive cave system that hosts saltwater vents and conspicuous microbial biofilm communities. For decades, water from Sulphur Springs has been extracted and used to supplement public water supply and dry season flows in the Hillsborough River Estuary. This dissertation describes research conducted at Sulphur Springs to determine the impacts of urban land use on the underlying cave and downstream estuary ecosystems, and presents the use of this system as a model to fill certain ecological knowledge gaps. …
Design And Implementation Of Degenerate Qpcr/Qrt-Pcr Primers To Detect Microbial Nitrogen Metabolism In Wastewater And Wastewater-Related Samples, Ryan F. Keeley
USF Tampa Graduate Theses and Dissertations
Nitrogen cycling processes can be tracked using quantitative Polymerase Chain Reaction (qPCR) to determine the presence and qReverse Transcriptase-PCR (qRT-PCR) to determine expression of key genes, or ‘biological markers’, for nitrogen metabolism. Nitrification is catalyzed in part, by two enzymes: ammonia monooxygenase (AMO; NH3 NH2OH) and nitrite oxidoreductase (NXR; NO2- NO3-). For denitrification, four enzymes act sequentially: nitrate reductase (NAR/NAP; NO3- NO2-), nitrite reductase (NIR; NO2- NO), nitric oxide reductase (NOR; NO N2O), and nitrous oxide reductase (NOS; N2O N2). A principle of wastewater treatment (WWT) is to remove excess nitrogen by taking advantage of natural nitrogen cycling …
Microbial Associations Of Four Species Of Algal Symbiont-Bearing Foraminifera From The Florida Reef Tract, Usa, Makenna May Martin
Microbial Associations Of Four Species Of Algal Symbiont-Bearing Foraminifera From The Florida Reef Tract, Usa, Makenna May Martin
USF Tampa Graduate Theses and Dissertations
Marine microbiome research is a rapidly expanding field of study, as scientists investigate the functions of microbial associations in eukaryotic organisms. Foraminifera are among the most abundant shelled organisms in the oceans, yet little is known of their associated microbiomes. This study investigated microbes associated with four species of Foraminifera that host three kinds of algal endosymbionts. The Order Miliolida, Family Soritidae, was represented by three species: Archaias angulatus and Cyclorbiculina compressa, which both host chlorophyte symbionts, and Sorites orbiculus, which hosts dinoflagellate symbionts. The fourth species, Amphistegina gibbosa, belongs to the Order Rotaliida and hosts diatom endosymbionts. Bacterial DNA …