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Full-Text Articles in Cell and Developmental Biology

Characterization Of Membrane Potentials In Vascular Smooth Muscle Of Hagfish, Lamprey And Trout, Prentiss Jones Jr. Aug 2006

Characterization Of Membrane Potentials In Vascular Smooth Muscle Of Hagfish, Lamprey And Trout, Prentiss Jones Jr.

Dissertations

The objective of this study was to characterize membrane potentials in systemic arteries of Pacific hagfish, Eptatretus stouti, Rainbow trout, Onchorhyncus mykiss, and Sea lamprey Petromyzon marinus. Previous studies have characterized membrane potentials in piscine tissue. However, these studies utilized non-vascular tissues such as cardiac muscle and skeletal muscle (69, 161). Characterization of membrane potentials in fish vasculature is without precedent in the literature. The hypothesis of this study was that membrane potentials in fish vascular smooth muscle differ little between species when comparable vasculature is evaluated.


Catalase Activity Mediates The Inhibitory Actions Of 24,25 Dihydroxyvitamin D3, Sven L. Peery May 2006

Catalase Activity Mediates The Inhibitory Actions Of 24,25 Dihydroxyvitamin D3, Sven L. Peery

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The steroid hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] rapidly stimulates the uptake of phosphate in isolated chick intestinal cells, while the steroid 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] inhibits the rapid stimulation by 1,25(OH)2D3. Earlier work in this laboratory has indicated that a cellular binding protein for the 24,25(OH)2D3 is the enzyme catalase. Since binding resulted in decreased catalase activity and increased H2O2 production, studies were undertaken to determine if pro-oxidant conditions mimicked the inhibitory actions of 24,25(OH)2D3, and anti-oxidant conditions …


Comparative Genomic Analyses And Expression Of A Set Of Overlapping Open Reading Frames Of Frog Virus 3, Type Species Of Ranavirus (Family Iridoviridae), Wendy Guat Hoon Tan Apr 2006

Comparative Genomic Analyses And Expression Of A Set Of Overlapping Open Reading Frames Of Frog Virus 3, Type Species Of Ranavirus (Family Iridoviridae), Wendy Guat Hoon Tan

Dissertations

Frog virus 3 (FV3) is the type species member of the genus Ranavirus (family Iridoviridae ). To better understand the molecular mechanisms involved in the replication of FV3, including transcription of its highly methylated DNA genome, the complete nucleotide sequence of the FV3 genome has been determined. The FV3 genome is 105, 903 base pairs long excluding the terminal redundancy. The G+C content of FV3 genome is 55% and it encodes 98 non-overlappingpotential open reading frames (ORFs) containing 50 to 1293 amino acids. Eighty-four ORFs have significant homology to known proteins of other iridoviruses, whereas 14 are unique FV3 ORFs. …