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Cell and Developmental Biology Commons™
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- <p>Autoimmune diseases – Research.</p> <p>Immunity – Regulations.</p> <p>Skin – Immunological aspects.</p> (1)
- <p>Enterobacteriaceae – Identification.</p> <p>Escherichia coli – Research.</p> <p>Pulsed-field gel electrophoresis.</p> (1)
- <p>Fernow Experimental Forest (W. Va.).</p> <p>Nitrifying bacteria.</p> <p>Soils – Nitrogen content – West Virginia.</p> <p>Watersheds – West Virginia.</p> (1)
- <p>Genetic disorders – Research.</p> <p>Pseudomonas aeruginosa.</p> <p>Pseudomonas aeruginosa infections – Research.</p> <p>Cystic fibrosis.</p> (1)
Articles 1 - 4 of 4
Full-Text Articles in Cell and Developmental Biology
Analysis Of E. Coli Dna From Selected Source(S) Using Pulsed-Field Gel Electrophoresis, Sheema Doshi
Analysis Of E. Coli Dna From Selected Source(S) Using Pulsed-Field Gel Electrophoresis, Sheema Doshi
Theses, Dissertations and Capstones
The presence of fecal coliforms in surface waters is a public health issue throughout the United States and Canada. The presence of Escherichia coli (E. coli) indicates recent fecal contamination from either animal or human sources. To solve this growing problem one must identify the source(s) of such contamination by creating a database of DNA profiles of£. coli isolates. Hence, tracking the source(s) of E. coli can help link water contamination to animal source(s) of contamination. In this study, pulsed field gel electrophoresis (PFGE) was used to analyze DNA from E. coli isolates from known animals. For PFGE, the bacterial …
Genomic Diversity Of Pseudomonas Aeruginosa In Cystic Fibrosis, Nathaniel Edwards Head
Genomic Diversity Of Pseudomonas Aeruginosa In Cystic Fibrosis, Nathaniel Edwards Head
Theses, Dissertations and Capstones
Pseudomonas aeruginosa is a common environmental microorganism. However, it has the genetic capacity to cause diseases in patients with compromised host defense systems. Cystic fibrosis (CF) is one of the major hereditary diseases among Caucasian populations. CF patients are born with a defective chloride channel that is responsible for maintaining fluid and electrolyte balance across the lumen of the lung. This imbalance leads to the production of an abnormal dehydrated viscous mucous, prohibiting the patient from normally clearing the respiratory airway. Chronic pulmonary infections with P. aeruginosa are the major causes of high morbidity and mortality in CF. Two of …
Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle
Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle
Theses, Dissertations and Capstones
The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …
Characterization And Regulation Of Skn-Directed Autoimmune Skin Lesions, Pamela Jane Staton
Characterization And Regulation Of Skn-Directed Autoimmune Skin Lesions, Pamela Jane Staton
Theses, Dissertations and Capstones
In the Skn model of autoimmunity, pathogenic T cells sensitized to Skn epidermal antigens (Skn-immune spleen cells or SIS cells) elicited disease in the form of skin lesions when adoptively transferred into shaved immunoincompetent syngeneic adult recipients (‘lesion-forming animals’) while concomitant injection of immunoregulatory normal spleen cells at twice the concentration (2 x normal spleen cells or 2XNS cells) of SIS cells significantly lessened lesion development (‘lesion-controlling animals’) by Day 7 post cell transfer. In a time-course RT-PCR cytokine analysis of skin from lesion-forming and lesion-controlling animals, IL-7 mRNA was significantly elevated in lesion-controlling skin on Day 7 while no …