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Full-Text Articles in Cell and Developmental Biology

Developmental Regulation Of A Cyclin-Dependent Kinase Inhibitor Controls Postembryonic Cell Cycle Progression In Caenorhabditis Elegans, Yang Hong, Richard Roy, Victor Ambros Aug 1998

Developmental Regulation Of A Cyclin-Dependent Kinase Inhibitor Controls Postembryonic Cell Cycle Progression In Caenorhabditis Elegans, Yang Hong, Richard Roy, Victor Ambros

Victor R. Ambros

C. elegans cki-1 encodes a member of the CIP/KIP family of cyclin-dependent kinase inhibitors, and functions to link postembryonic developmental programs to cell cycle progression. The expression pattern of cki-1::GFP suggests that cki-1 is developmentally regulated in blast cells coincident with G1, and in differentiating cells. Ectopic expression of CKI-1 can prematurely arrest cells in G1, while reducing cki-1 activity by RNA-mediated interference (RNAi) causes extra larval cell divisions, suggesting a role for cki-1 in the developmental control of G1/S. cki-1 activity is required for the suspension of cell cycling that occurs in dauer larvae and starved L1 larvae in …


The Temporal Correlation Of Changes In Apoplast Ph And Growth Rate In Maize Coleoptile Segments, Winfried Peters, Hartwig Lüthen, Michael Böttger, Hubert Felle Dec 1997

The Temporal Correlation Of Changes In Apoplast Ph And Growth Rate In Maize Coleoptile Segments, Winfried Peters, Hartwig Lüthen, Michael Böttger, Hubert Felle

Winfried S. Peters

Auxin induces extracellular acidification in growing shoot tissue. The causal relationship between this process and auxin-mediated growth is debated, partly because of contradicting previous reports on the temporal correlation of auxin-induced apoplast pH-drops and growth bursts. We have simultaneously measured both parameters on the background of spontaneously occurring endogenous changes in growth rate and apoplast pH in maize coleoptile segments. Our data demonstrate good temporal correlation, during both the ‘Spontaneous Growth Response’ and the response to exogenous auxin, which is transient under the conditions chosen due to rapid auxin metabolism. We suggest that cell wall pH and growth rate are …