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Gpu Deconvolution Wow Result On 2048x2048x32 Plane Z-Series, George Mcnamara
Gpu Deconvolution Wow Result On 2048x2048x32 Plane Z-Series, George Mcnamara
George McNamara
GPU Deconvolution WOW result on 2048x2048x32 plane Z-series ... formerly bad academic code ("you get what you pay for") now impressive
Alternative title: "instant gratification quantitative deconvolution fluorescence microscopy".
http://works.bepress.com/gmcnamara/55/
Please see "74"
http://works.bepress.com/gmcnamara/74/
for 32-bit images from this project (bepress file size limitation prevented me from including them in this ZIP archive).
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Summary: Deconvolution microscopy has historically been painfully slow. The early vendors were:
- Scanalytics (Carrington and Fay), commercialized to try to sell expensive, specialized array processors made by CSPI (the CSPI box likely had less computing power than a first gen smartphone).
- Applied Precision (Sedat …
Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara
Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara
George McNamara
GADPH single molecule mRNA FISH 20141104Tue
Raw data on NVidia TITAN GPU card deconvolution using original Bruce and Butte 2013 Optics Express software running inside Fiji ImageJ.
B&B GPU Deconvolution Reference:
http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766&id=249375
Acquired at 63x1x using leica plan apo 1.4 NA objective lens on Cooper lab Leica DMI6000 microscope, SOLA (initial release) LED lamp using only "green" LED, 554x Semrock exciter filter (in ASI ex wheel), Leica N3 (Cy3) filter cube, center 600 nm emission wavelength.
Hamamatsu FLASH4.0 sCMOS, 100 ms exposure time so 6.4 seconds acquisition time (64 planes X 4 seconds). Acquired with MetaMorph 7.8.8.
XY 100 nm …
Gpu Deconvolution Good Result On 2048x2048x32 Plane Z-Series (Update From Dataset 56), George Mcnamara
Gpu Deconvolution Good Result On 2048x2048x32 Plane Z-Series (Update From Dataset 56), George Mcnamara
George McNamara
GPU Deconvolution Good result on 2048x2048x32 plane Z-series (update from dataset 56)
This web page and download is
http://works.bepress.com/gmcnamara/62
Marc Bruce sent me the file here (web page 62) that is a preliminary bug fix (apparently of NVidia CUDA FFT code!!!) from the original 'raw' Z-series that is part of the
http://works.bepress.com/gmcnamara/56
web page download. Please see that web page for details on the project (GADPH single molecule mRNA detection with Stellaris FISH probe sets).
Marc is:
MARC BRUCE, PHD
CTO and Co-Founder
MICROVOLUTION, LLC
http://www.microvolution.com/
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The 730 Mb ZIP download includes 3 files:
* 32-bit TIFF series sent …
Fluorescence Microscopy Digital Deconvolution Comparison, George Mcnamara, Vinita Popat
Fluorescence Microscopy Digital Deconvolution Comparison, George Mcnamara, Vinita Popat
George McNamara
Presentation by Ms. Vinita Popat, Cornell University, 2013 summer student in Prof. Laurence J.N. Cooper lab, MD Anderson Cancer Center, Houston, TX. Project was evaluation of several deconvolution software for improving (or making worse!) fluorescence microscopy Z-series.