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Virginia Commonwealth University

Differential separation

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Full-Text Articles in Cell and Developmental Biology

Comparison Of Semi-Automated And Manual Differential Separation Methods For Mock Sexual Assault Samples, Stephanie Rink Jan 2021

Comparison Of Semi-Automated And Manual Differential Separation Methods For Mock Sexual Assault Samples, Stephanie Rink

Theses and Dissertations

In the event of a sexual assault, separation of suspect from victim DNA is possible through differential extraction if the evidentiary mixture consists of female epithelial cells and male spermatozoa. The basis of differential extraction is the differing properties of epithelial and sperm cell membranes, specifically the comparative sturdiness of the proteins making up the sperm head containing the male DNA. Effective retrieval and separation of male and female DNA fractions is important in generating high-quality STR profiles that can be utilized in identifying suspects in a sexual assault case. Differential extraction has been conventionally performed using Proteinase K (PK) …


Comparison Of Semi-Automated And Manual Differential Separation Methods For Mock Sexual Assault Samples, Stephanie Rink Jan 2021

Comparison Of Semi-Automated And Manual Differential Separation Methods For Mock Sexual Assault Samples, Stephanie Rink

Master of Science in Forensic Science Directed Research Projects

In the event of a sexual assault, separation of suspect from victim DNA is possible through differential extraction if the evidentiary mixture consists of female epithelial cells and male spermatozoa. The basis of differential extraction is the differing properties of epithelial and sperm cell membranes, specifically the comparative sturdiness of the proteins making up the sperm head containing the male DNA. Effective retrieval and separation of male and female DNA fractions is important in generating high-quality STR profiles that can be utilized in identifying suspects in a sexual assault case. Differential extraction has been conventionally performed using Proteinase K (PK) …