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Articles 1 - 3 of 3
Full-Text Articles in Cell and Developmental Biology
Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara
Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara
George McNamara
Timelapse data - GM imaging cytometer needs stitching part 2 of 2
Imaging challenge: align multiple time series over time to overcome "stage slop" in the imaging cytometer used in this experiment.
http://works.bepress.com/gmcnamara/53 (previous) and http://works.bepress.com/gmcnamara/54 (this)
are zip files containing 8-bit stacks (MetaMorph multiplane TIFF files) acquired on an imaging cytometer.
"53" is the brightfield data, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). "54" is GFP fluorescence, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). The GFP fluorescence decreases over time due to photobleaching. GFP disappears from cells when they die (soluble GFP diffusing …
Leica Microscope Gpu Deconvolution Stellaris Fish Dataset #1, George Mcnamara
Leica Microscope Gpu Deconvolution Stellaris Fish Dataset #1, George Mcnamara
George McNamara
McNamara 20131101Fri Leica widefield microscope CUDA Deconvolution Stellaris FISH probe cultured cells dataset #1.zip
A text file in the zip archive has experiment details. I am posting this online so that researchers - whether academic or commercial - can evaluate the acquired data, the Bruce and Butte 2013 Optics Express ( http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766 ) deconvolution result (note: I may not have used optimal settings), and to compare these deconvolution results to other methods. If anyone generates alternative spatial deconvolution output, such as from: * SVI Huygens * Media Cy AutoQuant * Agard's ER-Decon (Arigovindan 2013 PNAS) * Vicidomini SGP GPU Deconv …
Mcnamara 1987 Uiuc Phd Dissertation - Morphogenetic Movements Contributing To Cell Aggregation In Dictyostelium Discoideum Wild-Type And Streamer F Mutants, George Mcnamara
George McNamara