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Articles 1 - 4 of 4
Full-Text Articles in Cell and Developmental Biology
Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill
Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill
Jonathan Tan
Objective The nuclear factor-κB (NF-KB)/RelB transcription factor plays an essential role in development of some dendritic cell (DC) subsets in mice. In this laboratory, immature myeloid DC are produced in vitro in a stroma-dependent murine spleen long-term culture (LTC) system. In LTC, DC differentiate from hematopoietic progenitors maintained within the stromal cell matrix. Expression and function of RelB in development of LTC-DC has been investigated, with a view to assessing the relationship between DC produced in this system and other known subsets of DC. Materials and Methods RelB expression by LTC-DC was confirmed by detection of protein by Western blotting, …
Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill
Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill
Helen O'Neill
Objective The nuclear factor-κB (NF-KB)/RelB transcription factor plays an essential role in development of some dendritic cell (DC) subsets in mice. In this laboratory, immature myeloid DC are produced in vitro in a stroma-dependent murine spleen long-term culture (LTC) system. In LTC, DC differentiate from hematopoietic progenitors maintained within the stromal cell matrix. Expression and function of RelB in development of LTC-DC has been investigated, with a view to assessing the relationship between DC produced in this system and other known subsets of DC. Materials and Methods RelB expression by LTC-DC was confirmed by detection of protein by Western blotting, …
Cranial Ontogeny In Philautus Silus (Anura: Ranidae: Rhacophorinae) Reveals Few Similarities With Other Direct-Developing Anurans, Ryan Kerney, Madhava Meegaskumbura, Kelum Manamendra-Arachchi, James Hanken
Cranial Ontogeny In Philautus Silus (Anura: Ranidae: Rhacophorinae) Reveals Few Similarities With Other Direct-Developing Anurans, Ryan Kerney, Madhava Meegaskumbura, Kelum Manamendra-Arachchi, James Hanken
Ryan Kerney
Direct development has evolved in rhaco- phorine frogs independently from other anuran lineages, thereby offering an opportunity to assess features associ- ated with this derived life history. Using a developmen- tal series of the direct-developing Philautus silus (Ranidae: Rhacophorinae) from Sri Lanka, we examine features of cranial morphology that are part of a suite of adaptations that facilitate feeding in free-living tadpoles, but have been changed or lost in other direct-developing lineages. Larval-specific upper jaw cartilages, which are absent from many non-rhacophorine direct-developing species (such as Eleutherodactylus coqui), develop in embryos of P. silus. Similarly, lower jaw cartilages ini- tially …
Mycoplasma Contaminants Present In Exosome Preparations Induce Polyclonal B Cell Responses., Helen O'Neill, Ben Quah
Mycoplasma Contaminants Present In Exosome Preparations Induce Polyclonal B Cell Responses., Helen O'Neill, Ben Quah
Helen O'Neill
Exosome fractions of dendritic cells (DC) produced in long-term cultures (LTC) were found to contain Mycoplasma contaminants. In this study, Mycoplasma-infected, -uninfected, and -reinfected cultures of DC and control cell lines have been compared for their capacity to activate lymphocytes. Using differential centrifugation, size fractionation, and inhibition assays, it has been possible to map Mycoplasma to the exosome or vesicle fraction purified from culture supernatant (CSN). Mycoplasma fractions were shown to induce proliferation of B and not T cells. The B cell response was sensitive to mitomycin C and primaquine, both known antibiotics, but resistant to protease and DNase, suggesting …