Open Access. Powered by Scholars. Published by Universities.®

Cell and Developmental Biology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Physical Sciences and Mathematics

University of Dayton

Articles 1 - 2 of 2

Full-Text Articles in Cell and Developmental Biology

Effects Of Cell Cycle Noise On Excitable Gene Circuits, Alan Veliz-Cuba, Chinmaya Gupta, Matthew R. Bennett, Krešimir Josić, William Ott Nov 2016

Effects Of Cell Cycle Noise On Excitable Gene Circuits, Alan Veliz-Cuba, Chinmaya Gupta, Matthew R. Bennett, Krešimir Josić, William Ott

Mathematics Faculty Publications

We assess the impact of cell cycle noise on gene circuit dynamics. For bistable genetic switches and excitable circuits, we find that transitions between metastable states most likely occur just after cell division and that this concentration effect intensifies in the presence of transcriptional delay. We explain this concentration effect with a three-states stochastic model. For genetic oscillators, we quantify the temporal correlations between daughter cells induced by cell division. Temporal correlations must be captured properly in order to accurately quantify noise sources within gene networks.

Go to article

Confocal Microscopy: A Powerful Tool For Biological Research, Amit Singh, K. P. Gopinathan May 1998

Confocal Microscopy: A Powerful Tool For Biological Research, Amit Singh, K. P. Gopinathan

Biology Faculty Publications

Conventional light microscopy allows the observation of living as well as fixed cells and tissues to generate two-dimensional images. The out-of-focus information often obscures the ultrastructural details, especially in thick specimens with overlapping structures. The earliest available light microscopy visualized the objects in hydrated state in two-dimensions during their temporal development. The emergence of electron microscopy (EM) provided superb resolution of ultrastructural details, but it was applicable only for objects in the dehydrated state and thereby potentially introducing handling artifacts. The usefulness of optical methods, however, has been limited by the poor depth discrimination. Often, the fluorescence and reflectance images …

Go to article