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Full-Text Articles in Cell and Developmental Biology

Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst Jul 1998

Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst

Biological Sciences Theses & Dissertations

LifeNet currently cleans allograft bone utilizing AllowashTM Technology, which encompasses the use of a closed continuous circulation chamber to remove bone marrow by flushing it with AllowashTM Solution for a prescribed time and temperature after which, it is rinsed with various solutions to remove residual detergent that could be toxic to the recipient. A portion of this process employs AllowashTM Solution to improve the solubilization and removal of protein and cellular elements in the red bone marrow. This study examines the effectiveness of AllowashTM Solution in comparison to its component detergents Nonidet P40, Nonoxynol 9 and …


Molecular Mapping Of The Fatty (F) (Formerly Corpulent) Gene In The La/N Rat Obesity Model, Kenneth G. Butz Jan 1998

Molecular Mapping Of The Fatty (F) (Formerly Corpulent) Gene In The La/N Rat Obesity Model, Kenneth G. Butz

Theses, Dissertations and Capstones

The LA/N-corpulent (cp) rat obesity model was a congenic rat strain developed at the National Institutes of Health by C. T. Hansen . The LA/N-cp rat originated from breeding the Koletsky rat, possessing the corpulent gene, with the normotensive LA/N inbred rat. The autosomal recessive single gene mutation, cp, produces obesity with hyperphagia, hyperinsulinemia and elevated activities of liver and skeletal muscle lipogenic enzymes in the LA/N-cp rat model. The Zucker-fatty mutation, which was mapped to rat chromosome , resulted in a comparable, but not identical obesity syndrome. Breeding experiments by Yen et al …