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Full-Text Articles in Cell and Developmental Biology
Endocytic Intermediates Involved With The Intracellular Trafficking Of A Fluorescent Cellular Prion Protein, Ac Magalhaes, Ja Silva, Vr Martins, Vf Prado, Ssg Ferguson, Mv Gomez, Rr Brentani, Mam Prado
Endocytic Intermediates Involved With The Intracellular Trafficking Of A Fluorescent Cellular Prion Protein, Ac Magalhaes, Ja Silva, Vr Martins, Vf Prado, Ssg Ferguson, Mv Gomez, Rr Brentani, Mam Prado
Anatomy and Cell Biology Publications
We have investigated the intracellular traffic of PrPc, a glycosylphosphatidylinositol (GPI)-anchored protein implicated in spongiform encephalopathies. A fluorescent functional green fluorescent protein (GFP)-tagged version of PrPc is found at the cell surface and in intracellular compartments in SN56 cells. Confocal microscopy and organelle-specific markers suggest that the protein is found in both the Golgi and the recycling endosomal compartment. Perturbation of endocytosis with a dynamin I-K44A dominant-negative mutant altered the steady-state distribution of the GFP-PrPc, leading to the accumulation of fluorescence in unfissioned endocytic intermediates. These pre-endocytic intermediates did not seem to accumulate GFP-GPI, a minimum GPI-anchored protein, suggesting that …
Inducible Gene Expression In Cell Cultures And In Microencapsulated Cells, Yong Yu
Inducible Gene Expression In Cell Cultures And In Microencapsulated Cells, Yong Yu
Loma Linda University Electronic Theses, Dissertations & Projects
The mifepristone-regulated transcriptional activation system provides a means to exogenously control gene expression in transformed mammalian cells in a temporal and spatial fashion. Such a promoter activation system consists of three components: The first of these is a steroidal inducer drug, mifepristone. This drug binds to the second component, a chimeric transcription factor complex, consisting of the mutant human progesterone receptor fused to the yeast GAL4 DNA-binding domain and the Herpes simplex virus protein VP 16 activation domain. The third component is a synthetic promoter, consisting of a series of GAL4 recognition sequences upstream of the adenovirus major late E1B …