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Full-Text Articles in Cell and Developmental Biology
An Ultrastructural Survey Of The Nuchal Glands Of Rhabdophis Tigrinus (Serpentes: Colubridae), Kathleen A. Roberts
An Ultrastructural Survey Of The Nuchal Glands Of Rhabdophis Tigrinus (Serpentes: Colubridae), Kathleen A. Roberts
Biological Sciences Theses & Dissertations
Rhabdophis tigrinus, an Asian natricine snake, possesses a series of integumentary glands located in the nuchal skin. Previous studies indicated that the nuchal glands are of mesodermal origin, contain cardiac steroids analogous to bufotoxins found in the parotoid glands of toads, which are included in the snake's diet. The nuchal glands are defensive in function. Preliminary histological studies revealed differences in quality of the gland tissue compared to previous studies, and difference in fixation techniques were suspected. Seven specimens of R. tigrinus were fixed by perfusion using a conservative fixation process standard for electron microscopy. The nuchal gland series …
Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst
Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst
Biological Sciences Theses & Dissertations
LifeNet currently cleans allograft bone utilizing AllowashTM Technology, which encompasses the use of a closed continuous circulation chamber to remove bone marrow by flushing it with AllowashTM Solution for a prescribed time and temperature after which, it is rinsed with various solutions to remove residual detergent that could be toxic to the recipient. A portion of this process employs AllowashTM Solution to improve the solubilization and removal of protein and cellular elements in the red bone marrow. This study examines the effectiveness of AllowashTM Solution in comparison to its component detergents Nonidet P40, Nonoxynol 9 and …
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Biological Sciences Theses & Dissertations
Acid demineralized bone (DMB) implanted in extraosseous sites induces bone formation. In vivo studies have shown that DMB stimulates the differentiation of mesenchymal cells into osteogenic cells. In this study an in vitro system was developed to demonstrate the osteoinductivity of DMB quantitatively. This in vitro working system was used to assess the osteoinductivity of variously processed DMB products as a means to evaluate their processing protocols. A human dermal fibroblastic (HDF) cell line was initiated from tissue explants as a working model, along with a human periosteal (HPO) cell line as a control. Ground DMB processed by LifeNet Transplant …