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2020

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Articles 91 - 120 of 193

Full-Text Articles in Biotechnology

Design Of A 3d Printed Bioreactor For Bone Cancer Research, Brooklyn K. Vanderwolde, Katelyn Hillson May 2020

Design Of A 3d Printed Bioreactor For Bone Cancer Research, Brooklyn K. Vanderwolde, Katelyn Hillson

The Journal of Undergraduate Research

Bone cancer is an aggressive disease and has peak occurrence during physiological stimulation of growth and aging. Astronauts who undergo long-term space missions also acquire an increased risk of bone tissue degeneration and cancer. Few in-vitro models currently exist capable of reproducing the complex microenvironment of bone tissue to support multicellular activity in a three-dimensional structure. This limits the ability to understand disease progression and develop suitable treatment strategies. Therefore, developing a bone tissue model and an associated bioreactor is critical to understand the risks associated with cancer progression and improve treatment and preventions related to those risks. The goal …

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Circulating Micrornas In Neuromuscular Disorders, Nahla Mousa May 2020

Circulating Micrornas In Neuromuscular Disorders, Nahla Mousa

Theses and Dissertations

Identification of the mutations and genetic causes of the muscular diseases is performed for the muscular dystrophies, atrophies and other genetic diseases using current molecular high throughput techniques that precisely allocate the defected genes and detect the aberration in the expressed proteins responsible for any phenotype. For example, more than 2000 mutations in the DMD gene were identified in Duchenne Muscular Dystrophy patients or the milder form of the disease Becker Muscular Dystrophy. Detection of different mutation in the same gene has critical role as biomarkers in disease diagnosis, since the severity of the symptoms is usually associated with the …

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Using A Novel Cell- Penetrating Peptide Technology To Induce Pluripotency, Laura-Maria Oja May 2020

Using A Novel Cell- Penetrating Peptide Technology To Induce Pluripotency, Laura-Maria Oja

Master of Science in Chemical Sciences Theses

Somatic stem cells have been used as a regenerative medicine tool to treat diseases like leukemia for decades, but they are limited in their ability to self-renew and differentiate. A better alternative, induced pluripotent stem cells (iPSCs), has the ability to generate cells from all three germ layers and avoid tissue rejection as well as bypass the ethical concerns related to embryonic stem cells. In 2006, Takahashi and Yamanaka discovered that only four genes were required to induce pluripotency: Oct4, Sox2, Klf4, and c-Myc (OSKM). Since then, pluripotency has been induced with OSKM most effectively by transfection or transduction. However, …

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Understanding Covid-19 With Single Cell Immune Profiling, Alex Palacio May 2020

Understanding Covid-19 With Single Cell Immune Profiling, Alex Palacio

Creative Activity and Research Day - CARD

Scientists all around the world are coming together for a single goal -- to find the cure for COVID-19; the virus that has sent millions of people worldwide to hospitals and hundreds of thousands of people to their death. While scientists are trying to uncover the mysteries of the virus, 10X Genomics product lines are able to help scientists achieve their goal. With the Single Cell Immune Profiling, scientists around the world are able to study the immune response and antigen specificity of the virus. The Immune Profiling product line is able to study millions of T-Cells and B-Cells on …

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Chemoenzymatic Study Of Coa-Linked Rna In Bacteria, Krishna Sapkota May 2020

Chemoenzymatic Study Of Coa-Linked Rna In Bacteria, Krishna Sapkota

Dissertations

The ability of RNA to store genetic information and to catalyze biochemical transformations led to the speculation of the existence of RNA world before the evolution of contemporary ribonucleoprotein (RNP) world. Recent discovery of RNA molecules containing metabolic cofactors including coenzyme A and its various thioesters at their 5’ end further supported the RNA world hypothesis as these CoA-linked RNA molecules could be the molecular fossils with very ancient origin. As both RNA and Coenzyme A are believed to have co-existed since last universal common ancestor (LUCA) or even before, the CoA-RNA conjugates in current biology may reveal fundamental molecular …

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Optimization Of Rrna Depletion By Testing Various Kits, Alyssa Sancio May 2020

Optimization Of Rrna Depletion By Testing Various Kits, Alyssa Sancio

Creative Activity and Research Day - CARD

RNA sequencing (RNAseq) can be used to compare gene expression between different groups, identify mutations, and confirm gene knockouts. In RNAseq, RNA is extracted and built into a sequenceable library to analyze the transcriptome. Ribosomal RNA (rRNA) makes up roughly 90% of the total RNA extracted and does not provide useful transcriptome information, thus it must be removed in order to perform transcriptional analysis. At MBP Titan, the protocol used KAPA’s RiboErase Depletion kit using custom methanotroph oligonucleotides to specifically bind to rRNA for depletion. On average, the amount of rRNA remaining in the libraries ranged from 10-30%. Several depletion …

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Plasmid Dna Upstream Manufacturing Process Development, Shaquil Janif May 2020

Plasmid Dna Upstream Manufacturing Process Development, Shaquil Janif

Creative Activity and Research Day - CARD

The need to develop a robust method to produce large quantities of plasmid DNA has become vital due to the strides made in the field of gene therapy. More and more biopharmaceutical companies are starting to produce gene therapeutics to treat diseases at the molecular level. While the technology has been proven to work and success at treating disease using gene therapeutics have been made in clinical trials, a method to produce large quantities of DNA still remains a challenge. In this presentation, I will go over some of the current challenges associated with producing high copy numbers of plasmid …

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Antibody Binding Screens Using Biolayer Interferometry (Bli) And Surface Plasmon Resonance (Spr), Kerul Patel May 2020

Antibody Binding Screens Using Biolayer Interferometry (Bli) And Surface Plasmon Resonance (Spr), Kerul Patel

Creative Activity and Research Day - CARD

BLI and SPR are both label-free technologies being used to measure biomolecular interactions in real-time. Distributed Bio uses computational immunology techniques to generate antibody libraries against a target and bio-panning to identify and select clones that best interact with the target. The interaction is further tested using BLI and SPR technology platforms that enable determination of binding kinetics such as association and dissociation rates also known as on-rates and off-rates. Analysis of the on-rate and off-rate data is further used to determine and report the binding affinity of the target to the antibody clones which are then delivered to the …

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Hexadecane Petroleum, And Biofuel Utilization In Marine Bacteria Isolated From Ballast Tanks, Alex Yashchenko May 2020

Hexadecane Petroleum, And Biofuel Utilization In Marine Bacteria Isolated From Ballast Tanks, Alex Yashchenko

Graduate Theses

This study characterized the growth of bacteria isolated from ballast tank fluids in hexadecane, petroleum, plant, and algae-derived fuels. The study was performed to explore the capacity of ballast tank isolates to survive and grow within fuels that may be stored within ballast tanks. Results of the hexadecane analysis indicated that most isolates had higher viable cell counts in media supplemented with hexadecane. Members of Alteromonas, Pseudoalteromonas, and a single Brevundimonas species had viable cell counts that were one or several orders ofmagnitude greater than that of controls. Results offuel analysis indicated higher viable cell counts in pure JP-5 and …

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Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column, Ashley O'Brien May 2020

Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column, Ashley O'Brien

Biology Theses

In invertebrates gap junctions are formed by the innexin family of proteins. Remarkably, the genome of Hydra magnipapillata contains 17 innexin genes. This study focused on Hydra innexin-2 (h-Inx2) which is expressed in nerve cells and plays a role in contraction of the body column. The gene sequence of H-Inx2 was obtained from the National Center for Biotechnology Information (NCBI), the gene was synthesized externally and transferred to a vector suitable for expression in Xenopus oocytes (pcDNA3.1 CT-GFP TOPO). The TOPO CT-GFP vector includes a priming site for RNA polymerase which allows in vitro preparation of RNA. Another advantage is …

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Assessing The Influence Of Riparian Invasion By The Shrub Lonicera Maackii On Terrestrial Subsidies To Headwater Streams, Erin C. Rowekamp, Julia I. Chapman, Ryan Mcewan May 2020

Assessing The Influence Of Riparian Invasion By The Shrub Lonicera Maackii On Terrestrial Subsidies To Headwater Streams, Erin C. Rowekamp, Julia I. Chapman, Ryan Mcewan

Biology Faculty Publications

Invasion of Amur honeysuckle (Lonicera maackii) into riparian areas of headwater streams creates strong potential for alterations of terrestrial subsidies that may drive bottom-up effects on aquatic biota. In this study, we analyzed effects of L. maackii on terrestrial subsidies in stream sites that represented a gradient of invasion intensity in temperate deciduous forests of southwestern Ohio (USA). Leaf litter biomass, terrestrial and aquatic fine woody debris (0.5–9.9 cm diameter) volume and count, and terrestrial and aquatic coarse woody debris (>9.9 cm diameter) volume were analyzed. Based on previous research, we hypothesized that sites with a higher …

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A Crispr-Based Approach To Identify Host Factors Influencing Aav Production, Bryant Lowry May 2020

A Crispr-Based Approach To Identify Host Factors Influencing Aav Production, Bryant Lowry

Creative Activity and Research Day - CARD

Historically, the Adeno-Associated Virus (AAV) has been used as a vector for gene therapies due to its low pathogenicity, no known associated diseases, and clinical validity. However, transitioning from in-vitro AAV experiments to in-vivo prove challenging for both scalability and potency. It has been previously characterized that some host factors in human cells influence the packaging capabilities of the AAV vector. Therefore, with the assistance of Encoded Therapeutics, this project aims to validate previous efforts to identify human host factors that influence AAV production in cells with the intent to understand these mechanisms for therapeutic manufacturing benefit to lower AAV …

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Project Manager: Antibody Discovery At Distributed Bio, Pedro I. Justiniano May 2020

Project Manager: Antibody Discovery At Distributed Bio, Pedro I. Justiniano

Creative Activity and Research Day - CARD

Antibody discovery platforms have opened a new frontier in therapeutic development. Distributed Bio has built antibody libraries that produce computationally optimized antibodies pre-engineered with therapeutically desirable traits such as humanness, high CDRH3 diversity, thermostability, and other molecular scaffolding proven to pass clinical trial requirements. One of their most popular libraries is the Super Human 2.0 library. This library analyzes billions of antibodies to find the most suited antibody to bind to a specific antigen target. / As a Project Manager intern at Distributed Bio, I have been helping to manage projects of different companies to provide them with a product …

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Multiplexed Crispr Libraries For Cancer Functional Genomics, Jintan Liu May 2020

Multiplexed Crispr Libraries For Cancer Functional Genomics, Jintan Liu

Dissertations and Theses (Open Access)

High-throughput forward genetic screenings are invaluable tools to systematically explore genetic interactions and to link gene disruption with disease contexts. The adaptation of CRISPR/Cas9 has improved the sensitivity and specificity of functional screenings. Despite this advance, there remains a long-standing need to improve functional screenings with smaller and more versatile pooled libraries. Capitalizing on the inherent multiplexing capability of a class 2 CRISPR enzyme AsCpf1, we developed a multiplexed, high throughput screening strategy that has avoided the usual trade-off between library size and library penetration, allowing library minimization without sacrificing gene targeting efficiency. We optimized the AsCpf1 protein for functional …

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Editing Fusarium Graminearum Using Crispr/Cas9, Grace Marie Sack May 2020

Editing Fusarium Graminearum Using Crispr/Cas9, Grace Marie Sack

Honors Program Theses

Fusarium graminearum is a pathogenic fungus that causes scab or Fusarium head blight disease in barley and wheat. Alternative approaches to protect barley from this devastating disease are badly needed. In recent years, an RNA-guided genome-editing system known as clustered regularly interspaced short palindromic repeat and CRISPR-associated protein 9 (CRISPR/Cas9), has become a popular method for genome modification (Jinek et al., 2012). We applied CRISPR/Cas9 technology in an effort to make F. graminearum less infectious. We used CRISPR/Cas9 plasmids constructed by a former UNI student (Akers, 2019) to mutate three genes in F. graminearum. One of the genes ( …

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Direct Evidence Of Missing Mepsps Using Ca2-Sensor Imaging, Petar Gajic May 2020

Direct Evidence Of Missing Mepsps Using Ca2-Sensor Imaging, Petar Gajic

Biological Sciences

Following an action potential in the presynaptic neuron there is evoked release of neurotransmitter into the synapse which activates ionotropic transmembrane receptors on the postsynaptic membrane that cause depolarizations in voltage that get recorded as excitatory postsynaptic potentials (EPSPs). In the absence of an action potential there is spontaneous release of neurotransmitter that postsynaptically gets recorded as miniature excitatory postsynaptic potentials (mEPSPs). According to the quantal hypothesis, postulated by Bernard Katz, the mEPSPs are allor- none changes in potential caused by a single quantum of neurotransmitter, which when added up create EPSPs. Following studies have found that these two modes …

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Understanding Forest Fire Management In Indonesia From A Global Perspective, Riyanto Indra Agus, Cahyadi Ahmad, Kurniadhini Faricha, Bachtiar Hafidz, Apriyana Dwiki, Caraka Brian Kannardi Aji Apr 2020

Understanding Forest Fire Management In Indonesia From A Global Perspective, Riyanto Indra Agus, Cahyadi Ahmad, Kurniadhini Faricha, Bachtiar Hafidz, Apriyana Dwiki, Caraka Brian Kannardi Aji

ASEAN Journal on Science and Technology for Development

Forest fires are one of the global issues that attract worldwide attention. Russia, Brazil, Canada, the United States, and Indonesia are among the countries with the largest forest cover and long records of massive forest fires. Forest fire management is, therefore, critical to decreasing the severity level of these fires. Current conditions indicate that, compared with the four other countries, Indonesia has significantly reduced forest fires within the past five years. Consequently, adopting a global perspective to study the characteristics of forest fire disaster management has become necessary. For each management parameter, this research employed a literature review and descriptive …

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The Indonesian Throughflow And Its Impact On Biogeochemistry In The Indonesian Seas, Taufiqurrahman Edwards, Wahyudi A’An J., Masumoto Yukio Apr 2020

The Indonesian Throughflow And Its Impact On Biogeochemistry In The Indonesian Seas, Taufiqurrahman Edwards, Wahyudi A’An J., Masumoto Yukio

ASEAN Journal on Science and Technology for Development

It has been widely known that the Indonesian Throughflow (ITF) is an important inter-ocean connection with unique and complex oceanographic and geographic conditions, as well as a strong relation to both regional and global ocean currents and climate systems. Many studies on characteristics, mechanisms, and impacts of the ITF have been conducted, mainly focusing on the ITF pathways, transport, water mass mixing processes, and their variability in connection with monsoons and climate systems. In this paper, we summarize some of the critical aspects related to ocean conditions within the Indonesian Seas and the Indonesian Throughflow, with the main focus on …

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Anthropogenic Signatures In The Context Of Landscape Evolution: Evidence From Citanduy Watershed, Java, Indonesia, Malawani Mukhamad Ngainul, Mardiatno Djati, Haryono Eko Apr 2020

Anthropogenic Signatures In The Context Of Landscape Evolution: Evidence From Citanduy Watershed, Java, Indonesia, Malawani Mukhamad Ngainul, Mardiatno Djati, Haryono Eko

ASEAN Journal on Science and Technology for Development

The impact of humans on landscapes may differ across regions because anthropogenic intervention on the respective landscapes occurs in different human-induced contexts. This study was designed to assess the human-induced landscape evolution of the Citanduy Watershed, Java, Indonesia, and determine its anthropogenic signatures. Several approaches were used, namely anthropogenic landscape identification, anthropogenic soil development, sediment analysis, and monitoring of changes to Segara Anakan lagoon at the mouth of the Citanduy Rier. Five types of anthropogenic landscapes were observed in the research area, from a slightly modified landscape to a fully anthropogenic landscape. The anthropogenic signature was also found in the …

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Comparison Of 3d Coral Photogrammetry And Coral Video Transect For Coral Lifeform Analysis Using Low-Cost Underwater Action Camera, Ahmad Zuhairi Bin, Mohd Jinah Muhammad Idzham Helmi Bin, Saad Shahbudin Bin Apr 2020

Comparison Of 3d Coral Photogrammetry And Coral Video Transect For Coral Lifeform Analysis Using Low-Cost Underwater Action Camera, Ahmad Zuhairi Bin, Mohd Jinah Muhammad Idzham Helmi Bin, Saad Shahbudin Bin

ASEAN Journal on Science and Technology for Development

This research analysed the use of 3D Coral Photogrammetry (CP) and Coral Video Transect (CVT) images collected from SCUBA divers using a low-cost underwater action camera to examine the coral lifeform. A comparison was made between data sets obtained using both methods on nine transects with different coral lifeform compositions and percentage cover within an area of 4 × 7 m. The comparison of the statistical analysis for CPCe revealed that there were no significant differences (p < 0.05) between CP and CVT photos where dead corals (p = 0.006), sand (p = 0.011), and unknown (p = 0.002) are present. Additionally, the coral value (p = 0.131) between CP and CVT was not significant. CP was capable of producing prominent branching, massive, and plate coral morphology results. This suggests that survey methods using low-cost action cameras for 3D Coral Photogrammetry would yield appropriate results in terms of coral lifeform detection. Hypothetically, by improving camera quality, it will yield a higher accuracy of 3D coral images that are suitable for use in scientific research and management. Other benefits of using CP include the possibilities for future studies with 3D coral surveys using remotely operated vehicles, less field time, and 3D coral seabed information.

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Problem Solving Of Isopropyl Alcohol – Water Azeotropic Characteristics Using Packed (Natural Zeolite) Bed Adsorber, Prasakti Laras, Hartono Muhammad, Jati Pandu Prabowo, Setiaji Muhammad Fajar, Wirawan Sang Kompiang, Sudibyo Hanifrahmawan Apr 2020

Problem Solving Of Isopropyl Alcohol – Water Azeotropic Characteristics Using Packed (Natural Zeolite) Bed Adsorber, Prasakti Laras, Hartono Muhammad, Jati Pandu Prabowo, Setiaji Muhammad Fajar, Wirawan Sang Kompiang, Sudibyo Hanifrahmawan

ASEAN Journal on Science and Technology for Development

The adsorption kinetics of water from an azeotropic mixture of isopropyl alcohol and water using chemically activated natural zeolites with and without a binder (starch) was investigated. In addition, an investigation of the compressive strength of zeolite pellets was conducted to ascertain the performance of the adsorbent for long-term operation. Three parameters were applied in the zeolite-making process: the particle size of zeolite (20, 30, 50, and 80 mesh), the sintering temperature (550, 750, and 1,000°C), the compaction pressure (2, 4, and 6 tonnes), and the starch-to-zeolite weight ratio (0, 1:3, 1:5, and 1:7). Initial screenings indicated that the strongest …

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An Analysis Of Crispr-Cas Gene Editing In Agriculture, Ashley Laliberte Apr 2020

An Analysis Of Crispr-Cas Gene Editing In Agriculture, Ashley Laliberte

Honors Scholar Theses

The CRISPR-Cas system is a promising form of gene editing, especially for the agriculture industry. The ability to make single-nucleotide edits within a gene of interest, without the need to introduce foreign DNA, is a powerful tool for designing healthier and more efficient crops and food animals. This system provides opportunity for increased nutritional value, decreased food waste, and more economically and environmentally sustainable food production. Though this biotechnology is facing mechanistic limitations due to off-target effects and inefficient homology-directed repair, vast improvements have already been made to improve its efficacy. The CRISPR-Cas system is already the most advanced form …

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Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church, Mary Elizabeth Gresham Apr 2020

Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church, Mary Elizabeth Gresham

Senior Honors Theses

Infertility pushes the boundaries of emotional and physical health, which is why many couples inside and outside the church turn to Assisted Reproductive Technology (ART) for a solution. Despite what has seemed like silence from the Church, some individuals have braved the biological confusion and ethical dilemmas to evaluate the technology. Three major ethical viewpoints have emerged that each prioritize something over medical technology, namely community, order, or human dignity. This paper serves to educate pastors and church leaders on the ever-changing biology of ART as well as give voice to Christians that have spoken out on this issue. At …

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Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology, Jefferson Cypriano, Mounib Bahri, Kassioge Dembele, Walid Baaziz, Pedro Leao, Dennis A. Bazylinski, Fernanda Abreu, Ovidiu Ersen, Marcos Farina, Jacques Werckmann Apr 2020

Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology, Jefferson Cypriano, Mounib Bahri, Kassioge Dembele, Walid Baaziz, Pedro Leao, Dennis A. Bazylinski, Fernanda Abreu, Ovidiu Ersen, Marcos Farina, Jacques Werckmann

Life Sciences Faculty Research

Magnetosomes are intracellular magnetic nanocrystals composed of magnetite (Fe3O4) or greigite (Fe3S4), enveloped by a lipid bilayer membrane, produced by magnetotactic bacteria. Because of the stability of these structures in certain environments after cell death and lysis, magnetosome magnetite crystals contribute to the magnetization of sediments as well as providing a fossil record of ancient microbial ecosystems. The persistence or changes of the chemical and magnetic features of magnetosomes under certain conditions in different environments are important factors in biotechnology and paleomagnetism. Here we evaluated the thermal stability of magnetosomes in a temperature range between 150 and 500 °C subjected …

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The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense, Alyssa Forrest Apr 2020

The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense, Alyssa Forrest

Senior Honors Theses

Due to the dynamic nature of human conflict, non-traditional terror tactics have evolved to undermine the socioeconomic stability of targeted societies. Considering the landscape in which terrorists operate, emphasis on more subversive methods of biological terror have become prominent in recent decades. Agroterrorism, or the use of plant pathogens to infect a nation’s cultivated crops, is an emerging topic due to its threat to global food security and economic stability. Although emergency preparedness objectives have been enacted at national, state, and even local levels, preemptive measures can no longer remain the sole responsibility of intelligence and law enforcement agencies. The …

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Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems, Jake Dyess Apr 2020

Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems, Jake Dyess

Senior Honors Theses

The following thesis shall primarily explore the current research regarding the implementation of modifiable microscopic and nanoscopic polymer particles as novel pharmaceutical delivery systems. Polymethacrylate-based copolymers, such as EudragitTM, are sensitive to alterations in pH levels, becoming increasingly more soluble in response to an increase in pH. The selective solubility and modifiable nature of these polymers allows for greater flexibility of treatment options for patients (Patra et al., 2017). The wide range of functionality of polymethacrylate (PMA) may provide solutions to challenges relating to the current treatment and therapy of certain conditions. Research indicates that PMA based micro- …

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Optimasi Teknik Sterilisasi Dan Induksi Tunas Tanaman Durian (Durio Zibethinus Murr) ‘Kamajaya’ Lokal Cimahi Secara In Vitro, Agung Rahmadi, Noladhi Wicaksana, Bambang Nurhadi, Erni Suminar, Siti Rakhmah Tenrisui Pakki, Syariful Mubarok Apr 2020

Optimasi Teknik Sterilisasi Dan Induksi Tunas Tanaman Durian (Durio Zibethinus Murr) ‘Kamajaya’ Lokal Cimahi Secara In Vitro, Agung Rahmadi, Noladhi Wicaksana, Bambang Nurhadi, Erni Suminar, Siti Rakhmah Tenrisui Pakki, Syariful Mubarok

Jurnal Kultivasi

Durian ‘Kamajaya’ merupakan salah satu jenis durian lokal yang keberadaanya hampir punah sehingga perlu dilakukan konservasi, salah satunya yaitu dengan perbanyakan menggunakan kultur jaringan. Permasalahan yang muncul dalam kultur jaringan durian ini salah satunya adalah metode sterilisasi. Penelitian ini bertujuan untuk memperoleh metode sterilisasi yang tepat untuk perbanyakan tanaman durian ‘Kamajaya’ secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran, sejak bulan Mei sampai September tahun 2019. Eksplan yang digunakan adalah tunas muda dari tanaman durian ‘Kamajaya’ yang berasal dari Cimahi. Sterilisasi untuk inisiasi dengan teknik kultur jaringan menggunakan kombinasi air mengalir, detergen, …

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Role Of Microrna-208a As A Diagnostic And Prognostic Marker In Patients With St-Elevation Myocardial Infarction, Abou Bakr Mohamed Mohamed Hammad Salama Apr 2020

Role Of Microrna-208a As A Diagnostic And Prognostic Marker In Patients With St-Elevation Myocardial Infarction, Abou Bakr Mohamed Mohamed Hammad Salama

Theses and Dissertations

Acute Myocardial infarction (AMI) is the leading cause of death worldwide. Diagnosis of AMI depends on presenting symptoms, electrocardiogram (ECG), and cardiac troponins (cTns). Troponins are not good markers for patients presenting at the first hour of chest pain, so there is still a need for a marker that can be detected within the first hour of presentation, with high specificity and sensitivity, guide the medical decisions and give good insight on the expected prognosis. Previous studies reported miR-208a as a cardiac specific microRNA that can be detected in the plasma as early as 15 min of cardiac insult and …

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Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration, Meredith Cobb, Michaela J. Woods, Ryan Mcewan Apr 2020

Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration, Meredith Cobb, Michaela J. Woods, Ryan Mcewan

Biology Faculty Publications

In the Midwestern United States, there is a strong management impetus toward reforestation to replace trees lost to due to tree mortality and to establish forests on previously cleared properties. Here, we describe a public outreach and volunteer effort that aimed to generate positive, community-engaged restoration outcomes and report on a practical experiment involving seed handling. We obtained tree seeds that were donated by members of the community to local land managers. We evaluated the size of the seeds collected and tested the influence of common stratification media (none, sand and vermiculite) and seed size on germination success of three …

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Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage, Nafeesa Qudsia Hanif, Iffat Tahira, Nighat Sultana, Mujahid Hasan Mar 2020

Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage, Nafeesa Qudsia Hanif, Iffat Tahira, Nighat Sultana, Mujahid Hasan

Journal of Bioresource Management

An in vitro study was planned to assess the effects of a homofermentative microbial inoculant on the fermentation parameters and nutritive value of corn silage. The inoculant was applied at concentrations of 5x104cfu/g of forage (T1), 1x105cfu/g of forage (T2) 1.5x105cfu/g of forage (T3) and a negative control group (T0) without bacterial inoculant in three replicates each. At day 3, 7, 45 and 90 of the experiment individual buckets were opened to characterize the material, quick acidification, dry matter recovery, and aerobic stability of silage respectively. The temperature of the trial samples was 32.75±1.92 …

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