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Honors Theses

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Articles 1 - 10 of 10

Full-Text Articles in Biotechnology

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby May 2024

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby

Honors Theses

With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H2 that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …


Electrochemical Reduction Of Carbon Dioxide Using Ruthenium And Amines, Sydney Moise Apr 2023

Electrochemical Reduction Of Carbon Dioxide Using Ruthenium And Amines, Sydney Moise

Honors Theses

With an increased concern for climate change in the recent years, a significant area of research has been devoted to the reduction of greenhouse gases. Carbon dioxide (CO2) resides in the atmosphere between 300 – 1000 years, making the reduction of the molecule a substantial field of study.1 Amines have been used as CO2 scrubbing agents in literature historically, due to their ability to form bonds to carbon.2 Although studies involving metal catalysts and amines have been reported numerous times, research involving chemical reduction of CO2 using purely amines is scarce. In this paper, amines, in addition to hydride donors …


Biomechanics Of Mantis Prey Capture, Danielle S. Taylor Jan 2023

Biomechanics Of Mantis Prey Capture, Danielle S. Taylor

Honors Theses

Mantis species have a variety of different morphologies, so do the extreme forms of mantis limbs trade prey catching capability for camouflage? We hypothesize that some extreme forms of mantis limbs that are associated with cryptic species may be associated with a tradeoff of the capability of those limbs. Previous research has developed 2D morphologies of several hundred species of mantises. We are creating a 3D morphology by using micro dissection, micro CT imaging to construct our 3D biomechanical model. We found the attachment points of the ligaments and muscles from a Tenodera forearm and have constructed a 2D biomechanical …


Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez May 2021

Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez

Honors Theses

Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …


Use Of Co-Immunoprecipitations And 2d Gel Electrophoresis To Identify Protein-Protein Interactions Of Maturase K, Lauren Angello Dec 2018

Use Of Co-Immunoprecipitations And 2d Gel Electrophoresis To Identify Protein-Protein Interactions Of Maturase K, Lauren Angello

Honors Theses

Maturase K (MatK) is the only group II intron encoded protein in the chloroplast of land plants. Maturases are prokaryotic enzymes that catalyze formation of the lariat structure needed for intron removal from precursor RNAs. The chloroplast maturase MatK, is a descendant of prokaryotic maturases, however, unlike its prokaryotic relatives, MatK is thought to catalyze excision of, not only its own intron, but also the introns of other group II introns in the plastome. Similar to the multiprotein and snRNA spliceosomal complex of the nucleus, it is postulated that MatK is not working alone to excise these introns but most …


Control System For 3d Printable Robotic Hand, Htoo Wai Htet Jun 2016

Control System For 3d Printable Robotic Hand, Htoo Wai Htet

Honors Theses

Humanoid robotics is a growing area of research due to its potential applications in orthosis and prosthesis for human beings. With the currently available technologies, the most advanced robotic hands used in prosthetics or robotics can cost from $11,000 to $90,000, making it inaccessible to the general population of amputees and robotics hobbyists. Most of the features provided by these expensive technologies are superfluous to many users, creating a great gap in cost and services between users and technology. Using the emerging 3D printing technology, my project is to construct a 3D printed robotic hand that can reproduce as many …


Global Reach, Local Markets: The Challenges Of Leading Global Innovation, Sarah Higgins Jan 2016

Global Reach, Local Markets: The Challenges Of Leading Global Innovation, Sarah Higgins

Honors Theses

This Global Studies honors thesis addresses how managers and leaders of global firms manage innovation across multiple markets. Current research on multinational corporations provides an understanding of different kinds of innovation and the ways to attend to multiple markets. However, there is less documentation of how these innovation strategies are actually implemented on the ground and the tensions that these efforts might produce. Therefore, my research focuses in particular on the challenges and tensions faced by leaders of global firms as they implement transnational innovation strategies. This study is based upon in-depth interviews with 20 participants who held positions in …


Flow Cytometry And Biochemical Analysis Of Apoptotic Mouse Ht-2 T - Lymphocytes, Nell Pinkston Jun 2015

Flow Cytometry And Biochemical Analysis Of Apoptotic Mouse Ht-2 T - Lymphocytes, Nell Pinkston

Honors Theses

Apoptosis is a highly organized intracellular death program in multicellular animals. In the vertebrate immune system, apoptosis plays a central role in preventing the emergence of autoreactive lymphocytes. In this study, we used multiple stimuli (staurosporine, camptothecin, and cytokine deprivation - interleukin-2 or IL-2) to initiate apoptosis in IL-2 dependent, mouse HT-2 T-lymphocytes. All three inducers triggered DNA laddering and phosphatidylserine externalization. Propidium iodide staining and flow cytometry were also used to determine whether apoptotic cells accumulated in a specific stage of the cell cycle, and whether the mode of induction affected cell cycle distribution. Our findings indicate that IL-2 …


Understanding A Possible Wonder Drug: A Radial Diffusion Assay For The Rapid Evaluation Of Antimicrobial Peptides, Dustin Walter Apr 2015

Understanding A Possible Wonder Drug: A Radial Diffusion Assay For The Rapid Evaluation Of Antimicrobial Peptides, Dustin Walter

Honors Theses

Antimicrobial peptides (AMPs) have been a major research focus due to their potential to combat a variety of human pathogens. Our laboratory has identified several novel peptides that display significant antifungal activity. The effectiveness of these peptides in vitro has been promising; however, it has been shown that physiological concentrations of various salts along with other conditions are inhibitory to peptide activity. To further explore the inhibitory effects of these salts, a new assay was developed whereby we can observe the effects of various salts on the peptide killing activity. For our studies, we employed several clinical isolates of Candida …


Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres Aug 1993

Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres

Honors Theses

Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary …