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Articles 31 - 48 of 48
Full-Text Articles in Biotechnology
Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A. Hussein
Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A. Hussein
Student Works (2010-2019)
Conventional techniques for removing dissolved heavy metals are only practical and cost-effective when applied to high strength wastes with heavy metal ion concentrations greater than 100 ppm. The possibility of using a non-living algal biomass to solve this problem was studied. Lead(II) was used in this study because it has been reported to cause several disorders in human. The non-living algal biomass was obtained from the filamentous green alga Spirogyra neglecta. The effects of initial concentration and contact time, pH, and temperature on the adsorption of lead(II) by the non-living algal biomass were studied. The equilibrium isotherms and kinetics were …
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Student Works (2010-2019)
Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone …
A Study On Cell Suspension Cultures For Micropropagation And The Production Of Carpaine From Papaya (Carica Papaya L. Var. Eksotika)., Mohamad Fhaizal Mohamad Bukhori
A Study On Cell Suspension Cultures For Micropropagation And The Production Of Carpaine From Papaya (Carica Papaya L. Var. Eksotika)., Mohamad Fhaizal Mohamad Bukhori
Student Works (2010-2019)
Carica papaya L. is a member of Caricaceae family with four native genera. This fruit is economically important as in multi-purpose fruit produced for dessert and vegetable and is highly in demand for pharmaconomical trade particularly the plant compound as source of medicine. This species is a popular fruit crop in the tropical and sub-tropical regions of Malaysia, Thailand, Indonesia and the Philippines. It is also one of the most widely grown fruits in Malaysia and is mainly cultivated in Johor, Perak and Selangor. The Eksotika papaya variety was introduced to the farmers in the late eighties and this resulted …
Genetic Variability Studies In A Selected Population Of Azadirachta Excelsa (Sentang) With Aflp Marker Technology, Chiew Foan Chin
Genetic Variability Studies In A Selected Population Of Azadirachta Excelsa (Sentang) With Aflp Marker Technology, Chiew Foan Chin
Student Works (2000-2009)
A maternal half-s/b population of Azadirachta excelsa was established from seeds in a randomised complete block design with three replicates. 100 to 200 seeds were collected from each of the 13 selected mother trees. The mother trees were categorised into three groups i.e. large, medium and small according to their girth sizes. 50 progeny seedlings from each scedlot per replicate were planted in the experimental field. Genetic studies on phenotypic measurements and molecular marker analysis were carried out on the established A. excelsa population. Measurements of quantitative traits including diameter breast height (Dbh) and total height were made. Mean Dbh …
In Vitro Mutation Induction By Gamma Irradiation For The Improvement Of A Triploid Banana Pisang Berangan (Aaa), Shadia Aldelgadir Mohamed Rayis
In Vitro Mutation Induction By Gamma Irradiation For The Improvement Of A Triploid Banana Pisang Berangan (Aaa), Shadia Aldelgadir Mohamed Rayis
Student Works (2000-2009)
Tissue culture and induced mutation techniques were used to generate variation for the improvement of Pisang Bcrangan cv. 'Intan' (AAA). Best field perform ing banana in vitroplants were multiplied and evaluated for better field performance, which included improved agronomic traits such as earliness in fruiting, short plant stature, high yielding capacity with desirable fruit quality and tolerance to Fusarium wilt. A suitable micropropagation medium for Pisang Berangan was established through modification of MS basic medium, which resulted in significant increase in shoot multiplication. Shoot-tip meristems of Pisang Berangan were then irradiated at 20, 30, 40 and 60 Gy to induce …
Kajian Kultur Tisu Dan Aktiviti Sel Carnation (Dianthus Caryophyllus Linn.) Cv. Grenadin, Norfariza A. Kadir
Kajian Kultur Tisu Dan Aktiviti Sel Carnation (Dianthus Caryophyllus Linn.) Cv. Grenadin, Norfariza A. Kadir
Student Works (2000-2009)
Cellular behaviour in primary roots of standard length 11.15 ± 0.33 mm from four-day-old seedlings of Dianthus caryophyllus Linn. cv. Grenadin were studied in vivo and in vitro. Parameters such as mitotic index (MI). chromosome count the ploidy level, the mean cell and nuclear areas and the cell doub ling time (Cdt) were determined from 2 mm segments of root tips. The MI value of the root segment decreased when transferred from in vivo to Murashige and Skoog (MS) medium supplemented with 2.0 mg/l NAA. The mean chromosome count were generally stable during 6 months in culture period with 2n= …
Related Enzymatic Activities In Solid-Substrate Fermentation Of Sago Hampas With Pycnoporus Sanguineus, Rifat Ara Matta
Related Enzymatic Activities In Solid-Substrate Fermentation Of Sago Hampas With Pycnoporus Sanguineus, Rifat Ara Matta
Student Works (2000-2009)
Solid substrate fermentation (SSF) of sago pith residue known as 'hampas' with Pycnoporus sanguineus for enzyme production was carried out. Good growth of Pycnoporus sanguineus in SSF was noted on 'hampas' supplemented with 0.38% urea, 0.2% KH₂PO₄ and 0.05% MgSO₄·7H₂O over a period of 30 days. Xylanase, lignin peroxidase and laccase activities were detected in Pycnoporus sanguineus cultures. Maximum xylanase and laccase activities of 5.26U/g of substrate on day 10 of SSF and 14.35U/g on day 15 of SSF of substrate were obtained respectively. The lignin peroxidase activity was less pronounced with an activity of 1.31 U/g of substrate on …
Auditing And Biological Treatment Of Oleochemical Industry Waste, Fauziah Shahul Hamid
Auditing And Biological Treatment Of Oleochemical Industry Waste, Fauziah Shahul Hamid
Student Works (2000-2009)
The utilization of renewable resources particularly from plant-based industries, such as, in the oleochemical sector makes it possible for further expansion of down-stream process products. However, to remain competitive the pollution generated from this industry should not be taken lightly. Implementation of cleaner technology into the oleochemical process will increase the efficiency of resource utilization of the industry, concurrently reducing quantity of waste generated. Waste audit conducted at Cognis Oleochemical (M) (COM) Sdn. Bhd. indicated that the major process-wastes generated by the facility were glycerol residue (GR) from Cognis Rika (M) Sdn. Bhd. (CRM) and wastewater sludge (WWS) from COM …
Immobolisation And Biochemical Characterisation Of A Selected Commercially Available Laccase, Chandra Sundaralingam
Immobolisation And Biochemical Characterisation Of A Selected Commercially Available Laccase, Chandra Sundaralingam
Student Works (2000-2009)
ln this study, laccase from Novo Nordisk was chosen for biochemical characterization and immobilization investigation. The specific aims of this work was to develop optimum conditions for immobilization of laccase by entrapment method using copper alginate and characterize the laccase biochemically. The pH optimum of the crude enzyme extract was 5.5 and was found to be stable over a wide range of pH values. The apparent molecular weight of this enzyme was around 79,000 Daltons. Three peaks of activity (isoenzymes) were obtained from isoelectric focusing of pH values 4.7, 5.2 and 6.0. The optimum pH of the immobilized laccase by …
Isolation, Screening And Characterisation Of Lactic Acid Bacteria Isolated From Local Food Sources, Ahmad Faris Mohd. Adnan
Isolation, Screening And Characterisation Of Lactic Acid Bacteria Isolated From Local Food Sources, Ahmad Faris Mohd. Adnan
Student Works (2000-2009)
126 bacterial isolates were isolated from several local food sources. Out of these, 55 isolates were subsequently identified as lactic acid bacteria i.e. they were gram-positive and catalase negative. These 55 isolates were put through gel plug test to distinguish between homofermentative and heterofermentative isolates. 16 of the 55 lactic acid bacteria isolates were found to be homofermentative. From the 16 homofermentative isolates, seven were chosen on the basis of origin and sugars used in the enrichment process for preliminary physiological characterisation. Several conditions were chosen such as variable temperature, lactic acid tolerance, NaCl concentration and initial pH These serve …
Fixed Bed Biosorption Of Cu(Ii) By Immobilized Biomass Of Sargassum Baccularia, Kong Fei Tan
Fixed Bed Biosorption Of Cu(Ii) By Immobilized Biomass Of Sargassum Baccularia, Kong Fei Tan
Student Works (2000-2009)
The primary objective of this study was to investigate the feasibility of using the immobilized biomass of Sargassum buccularia. a brown marine macro algae. to remove and recover copper ions in a fixed bed reactor by passive adsorption. This is commonly referred to as biosorpliun. Inactivated biomass of S. baccularia were chemically modified b) immobilizing it onto polyvinyl alcohol (PVA} as a support matrix. Spherical gel beads that were produced in this manner were subjected to mass transfer and equilibrium studies. The biosorption of copper is a bi-phasic process. with an initial fast phase for the first 2 hours followed …
Production Of 2,3-Butanediol From Molasses, Isita Sengupta
Production Of 2,3-Butanediol From Molasses, Isita Sengupta
Student Works (2000-2009)
2,3-Butanediol is an important chemical, which is used in the manufacture of products such as synthetic rubber, printing inks, perfumes, fumigants, moistening and softening agents, explosives and plasticizers. It has been known as a bacterial fermentation product since early part of the 20th century. Klebsiella oxytoca Is an organism, which can convert a variety of sugars to 2,3-butanediol. Available literature shows that principal monosaccharides of cellulosic substances as well as major hemicellulosic compounds have been used as carbon source. However, there is very little information on the application of waste material as carbon source for fermentation of butanediol. In this …
Production Of Polyhydroaxyalkanoate (Pha) From Palm Oil By Pseudomonas Oleovorans And Indigenous Bacterial Isolate, Pseudomonas Sp., Zazali Alias
Student Works (2000-2009)
In this study, the yield of biomass and polyhydroxyalkanoate (PHA) of Pseudomonas oleovorans grown in batch and fed-batch cultivations were compared. When oleic acid (QA) (0.5% w/v) was used as carbon source, a significant increase in biomass.and PHA content were observed through fedbatch cultivation. The biomass and PHA content obtained from batch cultivation were 1.45±0.19 g/I and 2.50±0.77 % cell dry weight (CDW) respectively, while in the fed-batch cultivation, the biomass and PHA content increased significantly to 3.35±0.78 g/I and 32.72±0.30 %CDW respectively. When saponified palm olein (SPO) (0.5% w/v) was used as a carbon source, the batch cultivation yielded …
Urea Hydrolysis Using Immobilized Urease, Anita Arullan
Urea Hydrolysis Using Immobilized Urease, Anita Arullan
Student Works (2000-2009)
An inexpensive carrier matrix. vermiculite was used for immobilizing urease. A cost effective and simple method of coupling was developed using vermiculite. Immobilization of urease on Amberlite MB-land Nylon-6 tube were also studied. Activities of free and immobilized urease were studied and compared: Among all methods used for immobilization of urease. the method of coupling the enzyme to vermiculite gave the highest retained activity of 82% (Method I) and 89% (Method 2) at pH 6.0 and 5.5 respectively. Urease immobilized by Method I retained 69% and 30% of its original activity and Method 2 (using glutaraldehyde as coupling agent) retained …
Subcloning, Characterization And Sequencing Of The Streptomycin Resistance Gene Of A Multiple Antibiotic Resistance Transposon From Salmonella Typhi, Ramesvari Pararajasingam Pillai
Subcloning, Characterization And Sequencing Of The Streptomycin Resistance Gene Of A Multiple Antibiotic Resistance Transposon From Salmonella Typhi, Ramesvari Pararajasingam Pillai
Student Works (2000-2009)
Salmonella typhi S8 is resistant to ampicillin (Ac), chloramphenicol (Cm), cotrimoxazole (Ctm), streptomycin (Sm), and tetracycline (Tc). The Ac, Cm, Ctm. and Sm resistance traits have been shown to be mediated by a multiple antibiotic resistance transposon located on a large conjugative plasmid. Transposition of the transposon encoding Ac^R (ampicillin resistance), Cm^R Ctm^R and Sm^R designated TnX8, was done by Wong (1999) to a recipient replicon, plasmid pUB307. The Ac^R Cm^R, Ctm^R and Sm^R genes were subcloned into plasmid pKan by digesting the recombinant plasmid, des ignated pCL8 (pUB307::TnX8), with San. This subcloning strategy generated different recombinant pKans that harbour …
Antigenic Epitope Analysis Of The P101 Nucleocapsid Protein Of Human Herpes Virus 6 (Var.B), Katherine Anne Francis
Antigenic Epitope Analysis Of The P101 Nucleocapsid Protein Of Human Herpes Virus 6 (Var.B), Katherine Anne Francis
Student Works (2000-2009)
Human herpes virus 6 (HHV6) is an ubiquitous, lymphoproliferative P-herpesvirus, which infects almost all individuals during infancy and persists lifelong. Two variants of HHV6 (A and B) have been described. The present study is on HHV6B, which causes exanthem subitum, also known as roseola infantum, a common febrile childhood illness. Two methods of detection for anti-HHV6B in body fluids were used, being indirect immunofluorescence assay (IFA) and peptidylated pin-ELISA. For IFA, the target cells were HHV68-infected human cord blood mononuclear cells (HCBMC). Ninety eight infant sera were tested by IFA and all were found to be IgG-HHV6 positive. Seventeen breast …
Gas Chromatographic-Mass Spectrometric Analysis Of Captopril And Its Application In Pharmacokinetic Studies In Healthy Subjects, Zamri Chik
Student Works (2000-2009)
Captopril is a highly specific competitive inhibitor of the angiotensin-convening enzyme (ACE). This enzyme converts angiotensin I, a relatively inactive decapeptide, to angiotensin II , a potent endogenous vasoconstrictor substance. Captopril is widely used for the treatment of hypertension and congestive heart failure. This drug, which contains a sulphydryl group binds readily to albumin and to other plasma proteins. Captopril is unstable in blood and plasma ex vivo because of the reactivity of sulphydryl group which results in oxidation and rapid formation of disulphides. Therefore a fixative or a stabiliser must be added to each blood sample immediately following collection. …
Genetic Diversity Of Salmonella Typhi, Satheesh Nair
Genetic Diversity Of Salmonella Typhi, Satheesh Nair
Student Works (2000-2009)
Typhoid fever is a unique human septemic infection caused by Salmonella typhi. Typhoid fever is still an important public health problem in many developing countries. The continuing presence of the disease in endemic areas and emergence of multidrug- resistant strains in many developing countries as well as the increasing reports from developed countries has renewed the interest to better understand the epidemiology of typhoid fever and some aspects of its pathogenesis. Characterization of S. typhi strains in different epidemiological settings depends largely on the utility of highly precise molecular typing tools. A convenient, versatile and safe method for preparing bacterial …