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Articles 31 - 35 of 35
Full-Text Articles in Biotechnology
Detection Of Foodborne Pathogens By Micro-Filtration Using A Continuous Cell Concentrator Device, Klaire E. Jeffries, Eduardo Ximenes, Michael R. Ladisch
Detection Of Foodborne Pathogens By Micro-Filtration Using A Continuous Cell Concentrator Device, Klaire E. Jeffries, Eduardo Ximenes, Michael R. Ladisch
The Summer Undergraduate Research Fellowship (SURF) Symposium
Protecting consumers from foodborne illness is an important health concern facing the food industry today. An important deficiency exposed by foodborne illness is the inability to track contaminated food back to the source in a timely manner. Although there are established methods that detect bacterial pathogen contamination, they are limited in distinguishing viable bacteria reliably and quickly. Currently, food pathogen testing requires lengthy culture steps, which many times are delayed even longer due to the lack of in-house testing labs. Typically, two to three days elapses between when the food is sampled and the test results are available. This study …
Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus
Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Despite having progressed extensively in the field of synthetic biology in terms of DNA synthesis, analysis and transplanting, we still cannot reliably, quantitatively measure expression of new genetic constructs. We engineered a biobrick compatible expression cassette to control transcription and translation initiation which can be reused in new genetic contexts. Previous research has shown that the Bicistronic design have much lesser variations in expression with varying genes of interest as compared to the regular monocistronic design.(Mutalik, Endy, Guimaraes, Cambray, Lam, Juul, Tran & Paull, 2013) The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when combined …
Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao
Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao
The Summer Undergraduate Research Fellowship (SURF) Symposium
Engineering synthetic multicellular systems will lead to new synthetic biology technological platforms, inform developmental biology through recapitulation of natural systems and possibly unveil novel morphologies with practical applications not before reached throughout natural history (Maharbiz, 2012). Creating an exogenous molecular circuit that will polarize unicellular cells into “apical” and “basal” domains relative to a substrate plane would fulfill a missing component towards fully multicellular synthetic cellular communities (Maharbiz, 2012). To this end, a PIP3 polarization network previously designed by Chau and associates (Chau, Walter, Gerardin, Tang, Lim 2012) was coupled to the specific activation by niacin of a recombinant …
Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li
Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li
The Summer Undergraduate Research Fellowship (SURF) Symposium
In the study of metabolomics, one of the greatest challenges can be accurately identifying compounds detected in biological extracts, especially when standards are not readily available. Current metabolomic methods are also limited in that they provide little to no information about a compound’s metabolic origin. In this study, we sought to address these issues by developing a novel metabolomic method that employs stable isotope feeding, LC-MS, Xcms, and an analytical software algorithm to study the ‘phenylalanome’ of Arabidopsis thaliana. Using this approach we were able to develop a method that, based on current results, is capable of detecting over …
Fabrication Of Multilayered Structure For Coherent Random Lasing, John Rauchenstein, Young L. Kim
Fabrication Of Multilayered Structure For Coherent Random Lasing, John Rauchenstein, Young L. Kim
The Summer Undergraduate Research Fellowship (SURF) Symposium
High powered lasers have many applications, including medical treatment and surgery. However, these lasers are extremely expensive and are therefore not widely available. The aim of this study was to demonstrate a method to create such a laser with significantly decreased overall cost and increased efficiency. In order to do this, we explored a phenomenon called random lasing which is a light amplification process. To start with, a low-cost pumping laser is directed at normal incidence toward a multi-layered sample with two alternating layers. At first pearl, a naturally found material that has many organic nano-scale layers (similar to the …