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Full-Text Articles in Biology
Analysis Of Brevundimonas Subvibrioides Developmental Signaling Systems Reveals Unexpected Differences Between Phenotypes And C-Di-Gmp Levels, Lauryn Anne Sperling
Analysis Of Brevundimonas Subvibrioides Developmental Signaling Systems Reveals Unexpected Differences Between Phenotypes And C-Di-Gmp Levels, Lauryn Anne Sperling
Electronic Theses and Dissertations
The DivJ-DivK-PleC signaling system of Caulobacter crescentus is a signaling network that regulates polar development and the cell cycle. This system is conserved in closely related bacteria, including the sister genus Brevundimonas. Previous studies had shown unexpected phenotypic differences between the C. crescentus divK mutant and the analogous mutant of Brevundimonas subvibrioides, but further characterization was not performed. Here, phenotypic assays analyzing motility, adhesion, and pilus production (the latter characterized by a newly discovered bacteriophage) revealed that divJ and pleC mutants have mostly similar phenotypes as their C. crescentus homologs, but divK mutants maintain largely opposite phenotypes than …
Characterizing The Developmental And Reproductive Toxicities Of Cannabidiol And Δ9-Tetrahydrocannabinol, Dennis Ray Carty
Characterizing The Developmental And Reproductive Toxicities Of Cannabidiol And Δ9-Tetrahydrocannabinol, Dennis Ray Carty
Electronic Theses and Dissertations
Medical marijuana is legal in twenty-nine of the United States and an additional nineteen states have passed legislation for cannabidiol (CBD) treatment of drug-resistant epilepsy, such as Dravet Syndrome (DS) which is diagnosed in children as young as two months. While CBD has shown anecdotal and recently clinical trial efficacy in reducing seizure frequency in DS patients, little is known about the potential adverse side-effects on child physiology, brain development, adult disease, or subsequent generations. Moreover, CBD is rarely administered without including low concentrations of Δ9-tetrahydrocannabinol (THC). The goal of this project is to characterize the relative morphological, behavioral, reproductive, …
Effects Of Benzo[A]Pyrene And Cyp19a1b Knockdown On Zebrafish Development, Khalid M. Alharthy
Effects Of Benzo[A]Pyrene And Cyp19a1b Knockdown On Zebrafish Development, Khalid M. Alharthy
Electronic Theses and Dissertations
Benzo[a]pyrene (BaP) is a ubiquitous environmental contaminant that is both an endocrine disruptor and a carcinogen. Aromatase (CYP19) is a key enzyme in steroidogenesis playing a key role in the hypothalamus-pituitary-gonad feedback loop. We hypothesized that BaP would negatively impact cyp19a1b expression in zebrafish, in turn, adversely affecting development and physiology. Here, we consider whether the toxicities observed following BaP exposure are comparable to those following a transient morpholino (MO)-mediated CYP19a1b knockdown or exposure to an aromatase inhibitor (fadrozole) during early development. One-cell zebrafish embryos were injected with a CYP19a1b-MO or control-MO. Other non-injected embryos were exposed to nominal waterborne …