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Full-Text Articles in Biology
Metabolic Rescue Of “Glucose Addicted” Cancer Cells In Vitro, Paolo Vignali
Metabolic Rescue Of “Glucose Addicted” Cancer Cells In Vitro, Paolo Vignali
Pursuit - The Journal of Undergraduate Research at The University of Tennessee
Transformations in the glycolytic metabolism of neoplasms modulate their robust cellular division. This characteristic leads to an “addiction” to glucose for continued proliferation and viability. This study investigated whether glucose metabolites could rescue cellular viability in glucose-starvation conditions, a model of the inter-tumoral nutrient-deficient environment. Findings illustrated potential cellular viability rescue with pyruvate addition in glucose-deprived conditions, yet the same potential was not observed with lactic acid, a metabolite that exists at characteristically high concentrations within the intertumoral microenvironment. These results could implicate a predominance of certain metabolic pathways in nutrient-starved cells. Molecular transport capacities across plasma membranes are tied …
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Turkish Journal of Biology
The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. …