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Full-Text Articles in Biology
Screening Of The Small Molecule Library Of Meinox Enables The Identification Of Anticancer Compounds In Pathologically Distinct Cancers, Aynura Mammadova, Ari̇f Mermer, Fati̇h Kocabaş
Screening Of The Small Molecule Library Of Meinox Enables The Identification Of Anticancer Compounds In Pathologically Distinct Cancers, Aynura Mammadova, Ari̇f Mermer, Fati̇h Kocabaş
Turkish Journal of Biology
Small molecules are widely used for the modulation of the molecular basis of diseases. This makes them the perfect tool for discovering and developing new therapeutics. In this work, we have established a library of small molecules in house and characterized its molecular and druglike properties. We have shown that most small molecules have molecular weights less than 450. They have pharmaceutically relevant cLogP, cLogS, and druglikeness value distributions. In addition, Meinox's small molecule library contained small molecules with polar surface areas that are less than 60 square angstroms, suggesting their potent ability to cross the blood-brain barrier. Meinox's small …
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
Turkish Journal of Biology
Breast cancer is the most-diagnosed cancer type among women. The triple-negative subtype is an especially aggressive type of breast cancer. Although chemotherapy is almost the only option for the treatment of triple-negative breast cancer (TNBC), currently used chemotherapeutics are not effective enough, considering the poor survival rate of patients. Therefore, novel compounds need to be developed to improve survival rates. It has been known that quinonic compounds, which are found in nature, have antibacterial, antifungal, and antitumorigenic properties. Naphthoquinones are members of the quinone family and are widely used in research due to their promising properties. In this study, we …
Cytoprotective Effect Of A Functional Antipollutant Blend Through Reducing B[A] P-Induced Intracellular Oxidative Stress And Uva Exposure, Gi̇zem Örs, Sultan Gülçe İz
Cytoprotective Effect Of A Functional Antipollutant Blend Through Reducing B[A] P-Induced Intracellular Oxidative Stress And Uva Exposure, Gi̇zem Örs, Sultan Gülçe İz
Turkish Journal of Biology
Benzo[a]pyrene (B[a]P) is a ubiquitous environmental pollutant that reacts with skin and induces intracellular oxidative stress through reactive oxygen species (ROS) accumulation. The antipollution properties of natural extracts, especially including antioxidants, for inhibiting ROS in cells are gaining importance, in addition to the anticancer effects attributed to them. In this study, a commercial functional antipollutant blend of plant extracts consisting of ellagic acid standardized Punica granatum peel extract, Sambucus nigra fruit extract, Prunus cerasus seed extract, and hydrolyzed wheat protein with high antioxidant properties and UV damage-protective properties attributed to each one was investigated. The cytoprotective effect of this functional …
Peptidomic Characterization And Bioactivity Of Protoiurus Kraepelini(Scorpiones: Iuridae) Venom, Tuğba Doğan, Naşi̇t İğci̇, Ayşenur Bi̇ber, Seli̇n Gerekci̇, Hepşen Hazal Hüsnügi̇l, Afi̇fe İzbirak, Can Özen
Peptidomic Characterization And Bioactivity Of Protoiurus Kraepelini(Scorpiones: Iuridae) Venom, Tuğba Doğan, Naşi̇t İğci̇, Ayşenur Bi̇ber, Seli̇n Gerekci̇, Hepşen Hazal Hüsnügi̇l, Afi̇fe İzbirak, Can Özen
Turkish Journal of Biology
Protoiurus kraepelini is a scorpion species found in parts of Turkey and Greece. In this study, the peptide profile of its venom was determined for the first time. The electrophoretic profile of the crude venom showed a protein distribution from 2 to 130 kDa. MALDI-TOF MS analysis of the venom peptide fraction yielded 27 peptides between 1059 and 4623 Da in mass. Several ion channelblocking and antimicrobial peptides were identified by peptide mass fingerprinting analysis. Cytotoxic and antimicrobial effects of the venom were also demonstrated on Jurkat cells and Escherichia coli, respectively. As the first peptidomic characterization study on P. …
Comparative Evaluation Of Nano And Bulk Tin Dioxide Cytotoxicity On Dermal Fibroblasts By Real-Time Impedance-Based And Conventional Methods, Şükran Şeker
Turkish Journal of Biology
In this study, the possible cellular effects of tin dioxide (SnO2) nanoparticles, together with its bulk form, on mouse dermal fibroblasts (DFs) were revealed using in vitro assays. Particle characterizations were carried out with AFM, Braun-Emmet-Teller, and DLS analyses. The cells were treated with nano and bulk SnO2 at concentrations of 0.1, 1, 10, 50, and 100 μg/mL for 6, 24, and 48 h. At the end of the exposure periods, the morphology, viability, particle uptake, and membrane leakage statuses of the cells were evaluated. Furthermore, real-time monitoring of cell responses was performed by using an impedance-based label-free system. Findings …
The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
Turkish Journal of Biology
The MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plants are used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study, the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants (Hypericum adenotrichum, Salvia kronenburgii, and Pelargonium quercetorum) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231) using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to …
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Turkish Journal of Biology
The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that …
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Turkish Journal of Biology
The use of transition metal complexes as appropriate analogues to cisplatin and similar anticancer drugs nowadays is of utmost importance. The inertness of Pt(IV), Pd(II), and Rh(III) complexes and the choosing of convenient ligands could offer a possible advantage in comparison to Pt(II) complexes. These advantages could be attributed to the better anticancer activity and fewer possible side effects. In this paper, we have chosen three complexes of different transition metal ions, [(TLtBu)PdIICl]ClO4, [RhIII(LH2tBu)Cl3], and [PtIV(LH2tBu)Cl3]Cl (where TLtBu is 2,6-bis[(1,3-di-tert-butylimidazolin-2-imino)methyl]pyridine and LH2tBu is 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine), for biological tests. All three complexes exerted strong prooxidative and cytotoxic effects on the human colorectal …
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Turkish Journal of Biology
Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract and fractionated parts. Among the extracts/fractions, the …
Biocompatible Polymeric Coatings Do Not Inherently Reducethe Cytotoxicity Of Iron Oxide Nanoparticles, Ayli̇n Şendemi̇r Ürkmez, Ece Bayir, Eyüp Bi̇lgi̇, Mehmet Özgün Özen
Biocompatible Polymeric Coatings Do Not Inherently Reducethe Cytotoxicity Of Iron Oxide Nanoparticles, Ayli̇n Şendemi̇r Ürkmez, Ece Bayir, Eyüp Bi̇lgi̇, Mehmet Özgün Özen
Turkish Journal of Biology
Nanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. In this study, chitosan (CS)- and poly(acrylic acid) (PAA)-coated iron oxide nanoparticles (IONPs) were used to investigate the possible cytotoxicity of coated IONPs on model mammalian cell line SaOs-2 by evaluating cellular viability and membrane integrity. Increasing concentrations of IONPs increased the cytotoxic effect on SaOs-2 cells with both CS- and PAA-coated IONPs. Cell viability on day 3 …
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Turkish Journal of Biology
The presence of uninhibited side effects of cancer drugs often used in cancer treatment has stimulated the search for alternative therapeutic approaches. Therefore, anticarcinogenic effects of synthetic, herbal, and fungal drugs have been investigated for the treatment of various cancer types in recent studies. Lichens, symbiotic organisms of fungi and algae, synthesize metabolites with significant biological activities. The aim of the current study was to screen the anticancer potential of usnic acid on various types of nonmalignant cell lines (Vero, L929) and cancer cell lines (CaCo2, RD, Hep2C, HepG2, Wehi). The growth inhibitory effect of usnic acid was determined by …
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Turkish Journal of Biology
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant …
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Turkish Journal of Biology
Hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropyl cellulose (HPC), or hydroxypropyl methyl cellulose (HPMC) with silver nanoparticles were synthesized. They were analyzed and characterized using differential thermal analysis and thermogravimetry, atomic force microscopy, and static contact angle measurements. It was experimentally demonstrated that the silver-doped hybrid materials have pronounced antibacterial behavior by studying the reduction of Pseudomonas aeruginosa PAO1 biofilm formation on the tested materials. The results revealed biofilm reduction of 35.7% and 30% by SiO2/HPC/2.5% Ag and SiO2/HPMC/2.5% Ag hybrid materials, respectively, compared to the control. Cytotoxicity of examined materials and actin cytoskeleton organization of fibroblasts seeded on …
Apoptotic Induction Via Membrane/Dna Damage And Metabolic Inactivation By Synthetic Food Colorants In Allium Cepa Root Meristem, Prajitha Vazhangat, John Ernest Thoppil
Apoptotic Induction Via Membrane/Dna Damage And Metabolic Inactivation By Synthetic Food Colorants In Allium Cepa Root Meristem, Prajitha Vazhangat, John Ernest Thoppil
Turkish Journal of Biology
The present study investigates the cytotoxic and genotoxic effects of two frequently employed synthetic food colorants, lemon yellow and orange red, using an Allium cepa assay. Uptake of food colorants by root cells at different concentrations and varied exposures significantly altered the cytogenetic system, membrane integrity, mitochondrial function, root growth, and cell division. The food colorants induced significant DNA damage, micronucleus formation, and other severe chromosomal aberrations at genetic level and inhibited cell division as well as root growth. Severe DNA damage, membrane damage, and metabolic inactivation observed in the result are indicative of apoptotic cell death. These results confirm …
Cytotoxicity And Antiviral Activity Of Palladium(Ii) And Platinum(Ii) Complexes With 2-(Diphenylphosphino)Benzaldehyde 1-Adamantoylhydrazone, Vera Simic, Stoimir Kolarevic, Ilija Brceski, Dejan Jeremic, Branka Vukovic-Gacic
Cytotoxicity And Antiviral Activity Of Palladium(Ii) And Platinum(Ii) Complexes With 2-(Diphenylphosphino)Benzaldehyde 1-Adamantoylhydrazone, Vera Simic, Stoimir Kolarevic, Ilija Brceski, Dejan Jeremic, Branka Vukovic-Gacic
Turkish Journal of Biology
Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect …
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Turkish Journal of Biology
Capsaicin possesses cytotoxic/anticancer activity and shares some common structural features, including a benzene ring and a long hydrophobic carbon tail, with the histone deacetylase (HDAC) inhibitors trichostatin A and suberoylanilide hydroxamic acid. The aims of this study were to investigate HDAC inhibitory and cytotoxic activities of a synthetic derivative of capsaicin, hydroxycapsaicin (6-hydroxy-N-(4-hydroxy-3-methoxybenzyl)-8-methylnonenamide), in colon cancer cell lines. Hydroxycapsaicin inhibited HDAC activity in vitro (IC50 = 72 µM) much more effectively than the prototype capsaicin (IC50 > 13.1 mM) and also exhibited HDAC inhibitory activity in human cells (HeLa cells). MTT assay demonstrated that hydroxycapsaicin was less toxic than capsaicin against …
In Vitro Antiproliferative/Cytotoxic Activity Of 2,3'-Biindole Against Various Cancer Cell Lines, Sali̇h Ökten, Ramazan Erenler, Tuğba Kul Köprülü, Şaban Teki̇n
In Vitro Antiproliferative/Cytotoxic Activity Of 2,3'-Biindole Against Various Cancer Cell Lines, Sali̇h Ökten, Ramazan Erenler, Tuğba Kul Köprülü, Şaban Teki̇n
Turkish Journal of Biology
2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering …
In Vitro Cytotoxic, Genotoxic, And Oxidative Effects Of Acyclic Sesquiterpene Farnesene, Kübra Çeli̇k, Başak Toğar, Hasan Türkez, Numan Taşpinar
In Vitro Cytotoxic, Genotoxic, And Oxidative Effects Of Acyclic Sesquiterpene Farnesene, Kübra Çeli̇k, Başak Toğar, Hasan Türkez, Numan Taşpinar
Turkish Journal of Biology
Farnesene (FNS) is an acyclic sesquiterpene. It has a wide range of important biological effects such as antioxidant, antimicrobial, and antifungal properties, although its cytotoxic, cytogenetic, and oxidative effects have not been investigated in human blood tissue yet. To this aim, both MTT and lactate dehydrogenase (LDH) assays were carried out to evaluate cell viability and cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to assess oxidative alterations. In addition, micronucleus and chromosomal aberration tests were used for mutagenic and genotoxic studies. The results revealed that FNS reduced cell viability at concentrations of higher than …
Cytotoxic And Apoptotic Effects Of Ceranib-2 Offering Potential For A New Antineoplastic Agent In The Treatment Of Cancer Cells, Djanan Vejselova, Hati̇ce Mehtap Kutlu, Gökhan Kuş, Selda Kabadere, Ruhi̇ Uyar
Cytotoxic And Apoptotic Effects Of Ceranib-2 Offering Potential For A New Antineoplastic Agent In The Treatment Of Cancer Cells, Djanan Vejselova, Hati̇ce Mehtap Kutlu, Gökhan Kuş, Selda Kabadere, Ruhi̇ Uyar
Turkish Journal of Biology
Fibrocarcinomas are malignant tumours that originate in mesenchymal cells. The tumours typically form in the presence of connective tissue and are of key consideration when assessing research priorities for future healthcare management. Ceramidase inhibitors, such as ceranib-2, have demonstrated capacity to interfere with cellular DNA functionality, initiating apoptosis in many cancer cell lines. The enzyme ceramidase can regulate cellular levels of sphingosine and sphingosine-1-phosphate by controlling hydrolysis of ceramide. The present study investigates the antigrowth effects of ceranib-2 on mouse embryonic fibroblast cells (NIH/3T3 normal cell line) and rat embryonic fibroblast cells (5RP7 cancer cell line). Research was conducted using …
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Turkish Journal of Biology
The cytotoxicity of 6 highly cytotoxic Pseudomonas aeruginosa isolates was tested against Vero cells. Cytotoxicity was found to be cell-associated, high cytotoxicity was observed at a multiplicity of infection of 200:1, and this activity gradually decreased as the bacterial count decreased. This cytotoxicity caused rounding and detachment of the Vero monolayer as early as 1 h after infection. Moreover, viable metabolically active bacteria were necessary for Vero cell killing. These results imply that one or more of the type III secretion system effector proteins (ExoU, ExoS, ExoT, or ExoY) were responsible for the observed cytotoxic effects. This cytotoxicity was affected …
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Turkish Journal of Biology
The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. …
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Turkish Journal of Biology
Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diffusion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at IC_{50} = 85 ± 21.7 µg/mL. The best incubation time …
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Turkish Journal of Biology
Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.
Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta
Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta
Turkish Journal of Biology
The present experiment was undertaken to determine the mutagenic potential of lead on the cytomorphology of Trigonella foenum-graecum L. and to determine the maximum concentration of lead nitrate that induces maximum genetic variability. The seeds were treated with 6 different concentrations (10, 25, 50, 100, 200, and 400 ppm) of lead nitrate. The higher concentrations of lead nitrate significantly reduced plant height, number of branches per plant, pollen fertility, and yield; higher concentrations also caused variation in the plants at the seedling and mature stages. In addition, concentrations affected the pairing of homologous chromosomes and spindle formation. This resulted in …
Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan
Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan
Turkish Journal of Biology
The essential oils obtained from Anethum graveolens, Foeniculum vulgare collected at fully-mature and flowering stages, Mentha piperita, Mentha spicata, Lavandula officinalis, Ocimum basilicum (green- and purple-leaf varieties), Origanum onites, O. vulgare, O. munitiflorum, O. majorana, Rosmarinus officinalis, Salvia officinalis, and Satureja cuneifolia, as well as the widely encountered components in essential oils (\gamma-terpinene, 4-allylanisole, (-)-carvone, dihydrocarvone, D-limonene, (-)-phencone, cuminyl alcohol, cuminyl aldehyde, cuminol, trans-anethole, camphene, isoborneol, (-)-borneol, L-bornyl acetate, 2-decanol, 2-heptanol, methylheptane, farnesol, nerol, isopulegol, citral, citronellal, citronellol, geraniol, geranyl ester, linalool, linalyl oxide, linalyl ester, \alpha-pinene, \beta-pinene, piperitone, (-)-menthol, isomenthone, carvacrol, thymol, vanillin, and eugenol), were screened for their …
Cytogenetic Effects Of Fungicide Applications On Meiosis Of Tomato (Lycopersicon Esculentum Mill.), İlkay Öztürk Çali
Cytogenetic Effects Of Fungicide Applications On Meiosis Of Tomato (Lycopersicon Esculentum Mill.), İlkay Öztürk Çali
Turkish Journal of Biology
In the present study, the cytogenetic effects of the fungicide Agri Fos 400 (400 g/l mono- and di-potassium phosphonate) on meiosis of Lycopersicon esculentum Mill. (Family: Solanaceae) were studied. The fungicide was applied to tomatoes grown in greenhouse conditions at the dosage (400 ml/100 l water) recommended by the manufacturing company and double the recommended dosage (800 ml/100 l water). All dosages of the fungicide Agri Fos 400 caused various abnormalities in meiosis when compared with the control. This situation could lead to a decrease in the productivity of fruits.
Antibacterial, Antifungal And Cytotoxic Activities Of Tuberous Roots Of Amorphophallus Campanulatus, Alam Khan, Moizur Rahman, Shariful Islam
Antibacterial, Antifungal And Cytotoxic Activities Of Tuberous Roots Of Amorphophallus Campanulatus, Alam Khan, Moizur Rahman, Shariful Islam
Turkish Journal of Biology
Antibacterial, antifungal and cytotoxic activities of ethanol extract of tuberous roots of Amorphophallus campanulatus were studied. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The extract showed significant antibacterial activities against four gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Streptococcus b-haemolyticus) and six gram-negative bacteria (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhi). The MIC values against these bacteria ranged from 16 to 128 µg/ml. The antifungal …
Antiviral And Cytotoxic Activities Of Extracts From The Cell Cultures And Respective Parts Of Some Turkish Medicinal Plants, Atalay Sökmen
Antiviral And Cytotoxic Activities Of Extracts From The Cell Cultures And Respective Parts Of Some Turkish Medicinal Plants, Atalay Sökmen
Turkish Journal of Biology
Extracts from respective parts and cell cultures of some Turkish medicinal plants have been assessed for their antiviral and cytotoxic properties. None of the extracts tested showed notable activity against herpes simplex viruses (HSV-I and II), but a slight antiretroviral activity against HIV-I was determined in an extract from Hypericum capitatum cell cultures. On the other hand, according to cytotoxic activity test results against brine shrimp (Artemia salina), an activity level of LD_{50} at 34.9 µg/ml was found on the extracts of Ecbalium elaterium cell cultures.
Scanning Electron Microscopy And Transmission Electron Microscopy Aspects Of Synergistic Antitumor Activity Of Vitamin C - Vitamin K3 Combinations Against Human Prostatic Carcinoma Cells, Jacques Gilloteaux, James M. Jamison, Meenaxi Venugopal, David Giammar, Jack L. Summers
Scanning Electron Microscopy And Transmission Electron Microscopy Aspects Of Synergistic Antitumor Activity Of Vitamin C - Vitamin K3 Combinations Against Human Prostatic Carcinoma Cells, Jacques Gilloteaux, James M. Jamison, Meenaxi Venugopal, David Giammar, Jack L. Summers
Scanning Microscopy
A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K3 ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10-to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following …
Role Of Adhesion Molecules In The Mechanism Of Non-Mhc (Major Histocompatibility Complex) Restricted Cell-Mediated Cytotoxicity, Walter Malorni, Francesca Iosi, Daniela Zarcone, Carlo Enrico Grossi, Giuseppe Arancia
Role Of Adhesion Molecules In The Mechanism Of Non-Mhc (Major Histocompatibility Complex) Restricted Cell-Mediated Cytotoxicity, Walter Malorni, Francesca Iosi, Daniela Zarcone, Carlo Enrico Grossi, Giuseppe Arancia
Scanning Microscopy
Adhesion molecules involved in the interaction between immune system effector cells and tumor targets are surface molecules which contribute to the formation of cell-to-cell contacts and belong to the integrin family. In this paper, the role played by the adhesion molecules in the process of cell-mediated cytotoxicity is reviewed. Furthermore, the contact area between effector and target cells has been analyzed by scanning electron microscopy. This region, termed "closed chamber", seems to contribute to killing efficiency by creating an intimate contact region in which cytotoxic factors can easily induce lethal hit in target cell. Thus, the extension of the closed …