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Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Turkish Journal of Biology
A novel extracellular endoglucanase from a basidiomycete strain Peniophora sp. NDVN01 was purified 2.8-fold to homogeneity through ammonium sulfate precipitation and gel filtration with Bio-Gel P-100 and Sephadex G-75. The endoglucanase had a specific activity of 163.8 U/mg protein and a molecular mass of 32 kDa. Optimum temperature and pH were at 60 °C and 4.5, respectively. The enzyme was stable at up to 42 °C and in the pH range of 3.5-5.5 with a residual activity of over 80% for 24 h of treatment. Ni^2^+ activated but other metal ions showed no or slight inhibitory effect on the enzyme …
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Turkish Journal of Biology
In the present study, trehalase was purified and characterized from the seeds of Cicer arietinum L. Giza 1. Crude extract was prepared and purified for electrophoretic homogeneity using ammonium sulfate, chromatography on DEAE-cellulose, CM Sepharose, and Sephadex G-200. The final specific activity was 7 U/mg protein, with 232-fold purification. The purified enzyme exhibited its pH optimum at 5.5. The optimum temperature was 60 °C. The determined K_m value was 3.64 mM trehalose. The enzyme activity was stimulated by 20 mM Mn^{2+}, Ni^{2+}, or Co^{2+}, while it was inhibited by 20 mM Na^+, K^+, Li^+, Ca^{2+}, Zn^{2+}, Cu^{2+}, or Fe^{3+}. Zn^{2+} …