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Molecular Detection Of Antibiotic Related Genes From Pseudomonas Aeruginosa Fp6, An Antagonist Towards Rhizoctonia Solani And Colletotrichum Gloeosporioides, Sasirekha Bakthavatchalu, Srividya Shivakumar, Shankar Bhat Sullia
Molecular Detection Of Antibiotic Related Genes From Pseudomonas Aeruginosa Fp6, An Antagonist Towards Rhizoctonia Solani And Colletotrichum Gloeosporioides, Sasirekha Bakthavatchalu, Srividya Shivakumar, Shankar Bhat Sullia
Turkish Journal of Biology
Despite the importance of antibiosis in biological control, little is known about the genes involved in antifungal activity. Therefore, the present study was aimed at identifying the location of the antagonistic gene(s) in Pseudomonas aeruginosa FP6 towards Rhizoctonia solani and Colletotrichum gloeosporioides using a PCR-based approach. A new bacterial strain, designated as FP6, was isolated from rhizospheric soil and identified as a member of Pseudomonas aeruginosa based on 16S rRNA analysis. The secondary metabolites produced by this strain have shown broad-spectrum antifungal activity against Rhizoctonia solani and Colletotrichum gloeosporioides. Antifungal metabolites of a nonenzymatic nature were found to be responsible …
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Turkish Journal of Biology
Bacillus subtilis, isolated from the chili rhizosphere, capable of producing 3 mycolytic enzymes (chitinase, \beta-1,3-glucanase, and cellulase), was mutated by the chemical mutagen ethyl methane sulfonate (EMS). Mutants were screened based on their antifungal ability on dual plate assay against Colletotrichum gloeosporioides OGC1, the causal agent of anthracnose disease in fruit crops. EMS mutagenesis yielded 60 isolated mutants on NA plates, of which 3 (M3, M4, and M24) showed loss of antagonism against C. gloeosporioides OGC1 and 6 (M8, M21, M22, M57, M58, and M59) exhibited increased antagonism. The remaining mutants did not show any difference in their antagonistic property. …