Biology Commons^™

Antibiotic-Resistant Escherichia Coli And EnterococcusSpp. In Sand And Water At Tampa Bay Beaches, Jennifer K. Sabater

USF Tampa Graduate Theses and Dissertations

As antibiotic resistance in the environment continues to rise there is an increased concern that infections may become harder to treat as bacteria acquire genes for multidrug resistance. Recreational beach waters in the Tampa Bay area are routinely monitored by the Florida Department of Environmental Protection for the presence of fecal indicator bacteria (FIB) such as Escherichia coli and enterococci. Exceedances of beach action values (BAV) 235 CFU/100 mL (E. coli) and 70 CFU/100 mL (enterococci) indicate the presence of fecal contamination which is associated with an increased risk of disease for beachgoers. Antibiotic-resistant E. coli and Enterococcus spp. have …

Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven

USF Tampa Graduate Theses and Dissertations

Bolinopsis infundibulum is a wide-ranging, ubiquitous ctenophore whose fragile nature makes the collection of specimens and quantification of key predator-prey activities in controlled laboratory experiments, challenging. Thus, in situ methods often represent the best means for data collection. However, while present in surface waters, these animals can also be abundant at depths well beyond those attainable by divers. As a result, very little empirical data exist over the depth range of their natural habitats which limits our ability to assess key predator-prey interactions needed to assess their ecological role in midwater food webs. Working in Monterey Bay, California, remotely operated …

Shorebird Response To Human-Induced Changes At Three Pinellas County Beaches, Rebecca J. Ruthberg-Campagna

USF Tampa Graduate Theses and Dissertations

Worldwide, shorebird habitat is being destroyed and degraded by development and sea level rise. Shorebirds depend on availability of pristine, undisturbed coastal habitats for resting and feeding during migration as well as for reproduction. Migratory shorebirds using the East Atlantic Flyway visit the Gulf of Mexico Beaches of Pinellas County, Florida as a stopover site during Fall and Spring migration. In addition to hosting migratory species, Pinellas County beaches are home to several year-round resident species that breed during Summer. Pinellas County is the most densely populated county in Florida, and its Gulf Coast is heavily developed with commercial and …

The Use Of Spanish Moss As A Biological Indicator To Examine Relationships Between Metal Air Pollution, Vegetation Cover, And Environmental Equity In Tampa, Florida, Yousif Abdullah

USF Tampa Graduate Theses and Dissertations

Studies of inequality in exposure to less common air pollutants, like metals, are often limited by the costs of high spatial resolution measurements. Spanish moss (Tillandsia usneoides) is a promising bioindicator for measuring air pollution due to its lower cost, enabling capture of time-average environmental concentrations at high spatial resolution. This study had three major aims. First, I aimed to use Spanish moss as a bioindicator to characterize ambient concentrations of selected metals (Ti, Cr, Mn, Co, Ni, Cd, Hg, Pb, As, and Sb) in Tampa, Florida. My second goal was to determine the impact of vegetation cover on metals …

Spatiotemporal Changes Of Microbial Community Assemblages And Functions In The Subsurface, Madison C. Davis

USF Tampa Graduate Theses and Dissertations

The subsurface hosts diverse microbial community assemblages and functions. These communities play an important role in biogeochemical cycling and groundwater purification. Many physicochemical factors affect microbial communities and can cause short-term or long-term perturbations. Subsurface microbes are susceptible to anthropogenic changes in the environment, which can be caused by nutrient inputs or municipal groundwater extraction. Despite the importance of the subsurface microbiome, these microbial communities are poorly characterized. This dissertation describes the characterization of spatiotemporal drivers of subsurface microbial communities through a variety of techniques that include eDNA analyses, bioinformatics, hydrochemical analyses, stable isotope geochemistry, and multivariate statistics. Three coastal …

Isotope-Based Methods For Evaluating Fish Trophic Geographies, Julie L. Vecchio

USF Tampa Graduate Theses and Dissertations

Data on the movement and diets of fish during a variety of life stages are important inputs to fisheries stock assessments and marine ecosystem models. Stable isotopes may provide previously inaccessible information on movement and diet of a variety of managed and forage fish species. Here I used several novel means of interpretation for stable isotope data to infer diets and movements of several important fisheries species over both short (weeks to months) and long (lifetime) timescales. To calculate a constant partitioning offset (CPO) between the δ¹⁵N of muscle and of liver tissue, I conducted a literature search …

The Adaptations Of The Sulfur-Oxidizing, Gammaproteobacterium "Candidatus Endoriftia Persephone", Endosymbiont Of The Giant Tubeworm Riftia Pachyptila, To Hydrothermal Vent Habitat Heterogeneity, Juliana M. Leonard

USF Tampa Graduate Theses and Dissertations

The siboglinid tubeworm Riftia pachyptila is a dominant member of the deep-sea megafauna where seawater and hydrothermal vent (HTV) effluent interface and mix. It is one of the fastest growing invertebrates on land or in the sea. It does not have a digestive tract (e.g. mouth, gut, or anus), and is completely dependent on its sulfur-oxidizing endosymbiont, the Gammaproteobacterium “Candidatus Endoriftia persephone” for its nutritional requirements. This association was the first and is the most well studied among chemolithoautotrophic symbioses. “Ca. E. persephone” is a chemolithoautotrophic bacterium that oxidizes sulfide as an electron donor for energy, reduces oxygen as a …

The Distribution And Biogeochemistry Of Subtropical Intertidal Microbial Mats, Bert D. Anderson

USF Tampa Graduate Theses and Dissertations

Microbial mats have played an important role in the carbon (C) and nutrient cycles since the Archean Eon and modern mats are important contributors to the biogeochemistry of intertidal wetlands. Microbial mats are flat assemblages of microbes that are currently found in many unvegetated habitats globally. Intertidal salt pans are a common habitat for microbial mats, however little is known about the distribution of microbial mats within the intertidal landscape. Understanding the spatial distribution of microbial mats is critical to developing quantitative estimates of the impacts of microbial mats on their ecosystems. We photographically measured the presence and density of …

Species Abundance, Spatial And Vertical Distributionsof Large Heteropods (Pterotracheidae And Carinariidae)In The Northern Gulf Of Mexico, Kristine A. Clark

USF Tampa Graduate Theses and Dissertations

A description of species abundance, richness and distribution, and eye size of heteropod molluscs from the families Pterotracheidae and Carinariidae in the oligotrophic ecosystem of the northern Gulf of Mexico (GOM) is described based on discrete-depth sampling protocols. The collections were comprised from two midwater sampling programs conducted sequentially after the 2010 Deepwater Horizon oil spill (DWHOS): the Offshore Nekton Sampling and Analysis Program (ONSAP, 2011) and the Deep Pelagic Nekton Dynamics of the Gulf of Mexico (DEEPEND, 2015-2018). Study materials from DEEPEND were collected during the initial five cruises of 2015-2017. These programs collected over 3,495 heteropods in two …

Complex Tripartite Hydro Politics Of River Ganges, Muttaki Bin Kamal

USF Tampa Graduate Theses and Dissertations

This paper studies if the Flagship Namami Gange program of Cleaning the river Ganges in India aligns with the transcendental discourse on the river. Web contents as Facebook, YouTube and Twitter along with Indian English daily newspaper reports are used here as the source of data. The study shows that the program aligns with the transcendental discourse on river Ganges as the Mother Goddess and reaching out to her devotees.

Ecophysiology Of Oxygen Supply In Cephalopods, Matthew A. Birk

USF Tampa Graduate Theses and Dissertations

Cephalopods are an important component of many marine ecosystems and support large fisheries. Their active lifestyles and complex behaviors are thought to be driven in large part by competition with fishes. Although cephalopods appear to compete successfully with fishes, a number of their important physiological traits are arguably inferior, such as an inefficient mode of locomotion via jet propulsion and a phylogenetically limited means of blood-borne gas transport. In active shallow-water cephalopods, these traits result in an interesting combination of very high oxygen demand and limited oxygen supply. The ability to maintain active lifestyles despite these metabolic constraints makes cephalopods …

Quantitative Proteomics To Support Translational Cancer Research, Melissa Hoffman

USF Tampa Graduate Theses and Dissertations

Altered signaling pathways, which are mediated by post-translational modifications and changes in protein expression levels, are key regulators of cancer initiation, progression, and therapeutic escape. Many aspects of cancer progression, including early carcinogenesis and immediate response to drug treatment, are beyond the scope of genetic profiling and non-invasive monitoring techniques. Global protein profiling of cancer cell line models, tumor tissues, and biofluids (e.g. serum or urine) using mass spectrometry-based proteomics produces novel biological insights, which support improved patient outcomes. Recent technological advances resulting in next-generation mass spectrometry instrumentation and improved bioinformatics workflows have led to unprecedented measurement reproducibility as well …

Reef Fish Biodiversity In The Florida Keys National Marine Sanctuary, Megan E. Hepner

USF Tampa Graduate Theses and Dissertations

The biological diversity of reef-fish in the Florida Keys National Marine Sanctuary (FKNMS) from 1999 – 2016 was evaluated in terms of abundance, biomass, species richness, evenness, Shannon diversity, Simpson diversity, and functional diversity, using observations collected by multiple agencies and institutions under the Reef Visual Census (RVC) program. To compare the different diversity indices species richness, Shannon diversity, Simpson diversity, and functional diversity were converted into effective number of species. I examined the seven indices by no-take marine zones, in seven benthic habitat strata, and across the three-distinct geographic subregions in the Florida Keys domain (Upper, Middle, and Lower …

Spatial And Temporal Variation In Mangrove Distribution (1950-2014) In Tampa, Florida Usa, Carolyn Cheatham Rhodes

USF Tampa Graduate Theses and Dissertations

I carried out an observational study of historic high resolution aerial imagery spanning six decades (1950-2014) to identify recent and historic spatial extent of mangrove forests, within the municipal boundaries of the City of Tampa, Florida USA. My objectives were to map mangrove distribution and spatial extent and any change or patterns of change discernable. I observed variable patterns of change and rates of expansion varied between sites spatially as well as within sites between time intervals. I found notable changes in mangrove extent in the Tampa from historic and modern aerial imagery for the ~64-year period between 1950 and …

Land Use, Stream Stability, And Benthic Invertebrates In A Dry Forest Watershed Of Western Costa Rica, Jacqueline Ann Demko

USF Tampa Graduate Theses and Dissertations

There is a paucity of data on dry forests, the most threatened biome in the tropics. The Nandamojo is a tropical dry forest watershed in Western Costa Rica that has been impacted by varying degrees of human induced modifications. This research was conducted to examine the influence of land use and channel characteristics on invertebrate communities within a sub basin of the Nandamojo watershed. This study addressed three hypotheses: (1) sites with low tree cover and small riparian buffer zones will have high erosion, (2) macroinvertebrate abundance will be lower at sites with low channel stability values, and (3) benthic …

Population Dynamics Of The Little Gulper Shark (Centrophorus Uyato) And Community Analyses Of Elasmobranch Species In The Northern Gulf Of Mexico, Jacquelin Joye Hipes

USF Tampa Graduate Theses and Dissertations

In chapter 1 I describe the population dynamics of an understudied species of gulper shark, Centrophorus uyato (common name, the Little Gulper), found in the Northern Gulf of Mexico. Sharks in the family Centrophoridae are mid-sized, demersal fish, with seven species identified in North American waters. These deepwater species can be difficult to study due to the extreme depths at which they occur. During four longlining cruises from 2012-2014, 593 sharks were landed, predominantly in the Mississippi Canyon off the Louisiana coast. Mean depth of capture was 290 m. Supplementing these data are catch records for C. uyato from a …

Net Effects Of Batrachochytrium Dendrobatidis (Bd) And Fungicides On Anurans Across Life Stages, Jenise Brown

USF Tampa Graduate Theses and Dissertations

Amphibians are declining at alarming rates globally. Multiple factors contribute to these declines, including chemical contaminants and emergent diseases. In recent years, agrochemical use, especially fungicide applications, has increased considerably. Previous studies have demonstrated that these agrochemicals leave application sites and enter wetlands via runoff and have detrimental effects on non-target organisms. For example, exposure to contaminants can have multifarious effects on amphibians, such as reducing their ability to deal with a secondary stressor, such as disease.

A pathogen that is found concomitant with chemical contaminants in aquatic systems is Batrachochytrium dendrobatidis (Bd). Bd has decimated amphibian populations worldwide. Susceptibility …

A Study Of The Eastern Oyster, Crassostrea Virginica, In Tampa Bay: Effects Of Perkinsus Marinus On Reproduction And Condition, Bridgit Melora Mccrickard

USF Tampa Graduate Theses and Dissertations

Abstract

Five sites in Tampa Bay, Florida, were sampled monthly from February 2006 to January 2007. These sites were located at the mouth of the Alafia River, in an inlet of Cross Bayou, on the easement of Gandy Bridge, near mangrove in Manatee County, and an inlet of Salt Creek, off Bayboro Harbor. Standard methods were used to determine shell height and Condition Index (CI). Intensity and prevalence of Perkinsus marinus were analyzed using Ray's Thioglycollate medium test, while Haplosporidium nelsoni was studied by histological examination. Histological methods were also used to determine sex ratios, reproductive phases, and egg diameters. …

Responses To Chemical Exposure By Foraminifera: Distinguishing Dormancy From Mortality, Benjamin James Ross

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout in 2010 released an estimated 4.9 million barrels of crude oil into the Gulf of Mexico in the 83 days between the initial explosion and the capping of the well. Response included extensive use of Corexit© oil dispersant. Although South Florida was spared exposure by currents, this event highlights the need for effective bioassay organisms for coral reefs. Amphistegina spp. are benthic foraminifers that host diatom symbionts in a relationship similar to that of coral and their zooxanthellae. Amphistegina spp. occur abundantly in reef communities nearly worldwide, are easily collected and maintained in culture, and are …

Underwater Hearing In The Loggerhead Turtle (Caretta Caretta): A Comparison Of Behavioral And Auditory Evoked Potential Audiograms, Kelly Martin

USF Tampa Graduate Theses and Dissertations

Methods for collecting behavioral audiograms are often time consuming and require trained, captive subjects. It is more practical to measure hearing sensitivity using electrophysiological methods, such as auditory evoked potential (AEP) testing, in which electrodes measure action potentials in response to acoustic stimuli. These data can be collected in a matter of hours. However, results should be verified through behavioral testing. Current knowledge of marine turtle auditory abilities is based on a few electrophysiological tests. The purpose of this study was to collect and compare behavioral and auditory evoked potential audiograms in a captive adult loggerhead turtle (Caretta caretta …

A Novel Device For Cell-Cell Electrofusion, Justin T. Stewart

USF Tampa Graduate Theses and Dissertations

Cell transplantation therapy is a potentially powerful tool and can be used to replace defective cells with healthy cells. This offers the possibility of alleviating the destructive symptoms for many diseases such as Parkinson's disease, Alzheimer's disease, stroke, spinal cord trauma, Type I diabetes and many more. While there are many diseases that could be positively impacted from cell transplantation therapy, the focus of this research is insulin dependent, Type I Diabetes.

The Islets of Langerhans are composed of various types of cells located in the pancreas and are responsible for a variety of biochemical functions. Specifically, the beta Islet …

Evaluating The Effects Of Beach Nourishment On Loggerhead Sea Turtle (Caretta Caretta) Nesting In Pinellas County, Florida, Corey R. Leonard Ozan

USF Tampa Graduate Theses and Dissertations

The health of Florida's beaches are vital to the survival of loggerhead sea turtles (Caretta caretta), as nearly half of the world's loggerheads nest on the states beaches. Many of the beaches utilized by the turtles have undergone nourishment projects in hopes of combating erosion of the shoreline, protecting beachfront property, and creating more suitable beaches for tourism. Although it is argued that beach nourishment benefits sea turtles by providing more nesting habitat, the effects of the Pinellas County nourishment projects on loggerhead nesting are unknown. Beach nourishment can alter the compaction, moisture content, and temperature of the sand, all …