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Genetic Variation Of Northern Long-Eared Bat (Myotis Septentrionalis) Populations In Nebraska, Michael Palandri, Jeremy White May 2018

Genetic Variation Of Northern Long-Eared Bat (Myotis Septentrionalis) Populations In Nebraska, Michael Palandri, Jeremy White

Theses/Capstones/Creative Projects

The objective of this experiment was to determine genetic variation between Nebraska populations of Northern Long-eared Bats (Myotis septentrionalis) and to relate this variation to White-Nose Syndrome resistance. We hypothesized that these populations would show variation due to spatial separation and a lack of intermittent hibernacula. Wing tissue samples were collected from different areas in Nebraska including Ponca State Park, Fontenelle Forest, Elm Creek, and Larrabee Creek. A total of 31 specimens were caught between these locations. Mitochondrial DNA was extracted, amplified, and run on a gel using 6 samples from Ponca State Park. The gel from the …


Analyzing The Effects Of Site-Directed Mutagenesis On The Structure Of Coxsackievirus B3 Genomic Rna, Erin Dimon May 2018

Analyzing The Effects Of Site-Directed Mutagenesis On The Structure Of Coxsackievirus B3 Genomic Rna, Erin Dimon

Theses/Capstones/Creative Projects

Coxsackievirus B3 (CVB3) is known to cause myocarditis and pancreatitis in humans. The virus has a single stranded RNA genome that codes for 11 different proteins. CVB3 is found to have two serotypes: 28 (virulent and disease causing) and GA (avirulent and not disease causing). Like other members of the Picornaviridae family, CVB3 utilizes the genomic 5’ untranslated region (5’ UTR) to initiate viral replication mechanisms through interactions with host protein factors. Structural variations of the CVB3 5’ UTR are hypothesized to influence the success of such interactions and consequentially determine viral virulence. The aim of this project was to …