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Articles 1 - 8 of 8
Full-Text Articles in Biology
The Emerging Oral Pathogen, Filifactor Alocis, Modulates Antimicrobial Responses Of Primed Human Neutrophils., Ian J. Snider, Aruna Vashishta, Richard J. Lamont, Silvia M. Uriarte
The Emerging Oral Pathogen, Filifactor Alocis, Modulates Antimicrobial Responses Of Primed Human Neutrophils., Ian J. Snider, Aruna Vashishta, Richard J. Lamont, Silvia M. Uriarte
The Cardinal Edge
Almost 50% of the adult population older than 30 years of age suffers from some form of periodontitis, a chronic inflammatory disease of the periodontal tissue caused by microbial subversion of the host immune response. Neutrophils are the most abundant leukocyte present in the oral mucosa. In periodontitis, periodontal pathogens have developed strategies to evade neutrophil antimicrobial responses and promote bacterial growth. Among these oral pathogens is Filifactor alocis which can modulate neutrophils’ antimicrobial responses by preventing phagosome maturation. During inflammation, neutrophils that reach the gingival tissue are primed by cytokines and chemokines. However, the response of primed human neutrophils …
Effects Of Osmotic Stress On Dna And Cell Viability In A Desiccation-Sensitive Cell Line, Brady D. Russell, Rajib Hassan, Michael A. Menze
Effects Of Osmotic Stress On Dna And Cell Viability In A Desiccation-Sensitive Cell Line, Brady D. Russell, Rajib Hassan, Michael A. Menze
The Cardinal Edge
Kc167 is a widely used Drosophila cell line, known to be sensitive to the extreme water loss caused by desiccation. In order to characterize the effects of this desiccation-sensitivity on DNA and cell viability, a series of osmotic stressors of differing concentrations were introduced to the cell line. These cells were then imaged via the Cytation1 cell imaging machine using fluorescence microscopy. Specifically, cells were stained using the DAPI staining solution, a blue fluorescent DNA stain that binds strongly to A-T rich regions within the DNA, forming a fluorescent complex. As DAPI more readily enters the membrane and thereby stains …
Resolving Commingling, Restoring Identity: An Interdisciplinary Collaboration And Ethical Study Of Individuals From A Human Skeletal Teaching Collection, Morgann L. Lucas, Morgan J. Elmore, Christine Chen, Carolann Cockerill, Mekenzie Davis, Vivian N. Pham, Matthew Kolmann, Linda Fuselier, Kathryn E. Marklein
Resolving Commingling, Restoring Identity: An Interdisciplinary Collaboration And Ethical Study Of Individuals From A Human Skeletal Teaching Collection, Morgann L. Lucas, Morgan J. Elmore, Christine Chen, Carolann Cockerill, Mekenzie Davis, Vivian N. Pham, Matthew Kolmann, Linda Fuselier, Kathryn E. Marklein
The Cardinal Edge
In Fall 2022, human skeletal remains were discovered in the Department of Biology’s Comparative Vertebrate Anatomy Laboratory. No documentation about the acquisition or curation history was found. With no current protocols for repatriating individuals in university skeletal teaching collections, an interdisciplinary research team analyzed the skeletal remains to resolve to commingle and identify the people. Using standardized methods in forensic anthropology, we estimated the minimum number of individuals represented through taphonomic, demographic, paleopathological, and morphological variables and variation. Results indicated, minimally, 36 to 56 individuals represented by 250 bones. Of these individuals, 12 were estimated as probable female, 16 as …
Histological Evaluation Of Offspring Kidneys Following Prenatal Vaping, Lucas Georges, Katelyn Chism, Selma Podbicanin, Isaiah Burciaga, Cynthia Corbitt, Rachel Neal
Histological Evaluation Of Offspring Kidneys Following Prenatal Vaping, Lucas Georges, Katelyn Chism, Selma Podbicanin, Isaiah Burciaga, Cynthia Corbitt, Rachel Neal
The Cardinal Edge
Pregnant women are advised to switch from cigarettes to electronic cigarettes to reduce the toxic effects on the fetus, however little research has been done on the impact of vaping on child development. In this study investigating the impact of inhalation exposure to a commercial product on reproductive and developmental functions, C57BL/6 dams were exposed to vapor from VuseTM e-cigarette golden tobacco pod for 3 hours/day at two puffs per minute throughout gestation (Vape) or to filtered air (Sham). This resulted in smaller litter sizes, along with greater weights for Vape offspring at birth that returned to average by …
The Impact Of Prenatal Vape Exposure On Weanling Liver Gene Expression, Isabella R. Fieger, Cristina Isabel Pauig, Mindy Yin, Anna Lipinski, Barrett Blevins, Tyler Plake, Lucas Georges, Katelyn Chism, Isaiah Burciaga, Selma Podbicanin, Cynthia Corbitt, Rachel Neal
The Impact Of Prenatal Vape Exposure On Weanling Liver Gene Expression, Isabella R. Fieger, Cristina Isabel Pauig, Mindy Yin, Anna Lipinski, Barrett Blevins, Tyler Plake, Lucas Georges, Katelyn Chism, Isaiah Burciaga, Selma Podbicanin, Cynthia Corbitt, Rachel Neal
The Cardinal Edge
The use of E-cigarettes is a fairly recent phenomenon. Vaping is seen as the “healthier” alternative to smoking cigarettes, yet we know little about the developmental toxicity of commercially available vaping products. In the current study, C57Bl/6 mice were exposed to Vuse Alto Golden Tobacco pods (5% nicotine) 4 days before mating and throughout gestation (GD19) for 1 hour/day every day. Offspring birth outcomes were measured with liver tissue collected at weaning. Gross histology and gene expression in the SIRT1-FXR pathway were examined via qPCR analysis with male and female offspring analyzed separately. No differences in gross morphology or cell …
Characterization Of Oral Pathogen, Filifactor Alocis, And Its Virulence Factors That Contribute To The Progression Of Periodontitis., Ian J. Snider
Characterization Of Oral Pathogen, Filifactor Alocis, And Its Virulence Factors That Contribute To The Progression Of Periodontitis., Ian J. Snider
The Cardinal Edge
No abstract provided.
Targeting Of The Hedgehog Signaling Pathway In Cancer Treatment, Andrew J. Hawes
Targeting Of The Hedgehog Signaling Pathway In Cancer Treatment, Andrew J. Hawes
The Cardinal Edge
The Hedgehog (Hh) signaling pathway is a developmental pathway that is highly conserved evolutionarily. While typically only displaying high activity during embryogenesis, overactivation of the Hh pathway in adults has been linked to multiple forms of cancer including acute myeloid leukemia, myelofibrosis, basal-cell carcinoma, pancreatic ductal adrenal carcinoma, and triple negative breast cancer. The prevalence of Hh activation in many different cancers has made it a prime target for inhibition of these cancers through novel therapies. This literature review sought to assess the current state of cancer treatment through inhibition of Hh signaling. Most current clinical trials involving the pathway …
The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running
The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running
The Cardinal Edge
Prenylation is a type of post-translational modification in which a 15- or 20-carbon lipid is added to the carboxyl (C) terminus of the protein. Arabidopsis thaliana contains the PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, which encodes a protein with homology to the α-subunits of the three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations in PPAL, one of which is ppal-1, which contains a T-DNA insertion in the fourth intron. We have previously observed that self-fertilizing heterozygous ppal-1 plants produce progeny in which homozygous ppal-1 is underrepresented. This project attempts to ascertain …