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Full-Text Articles in Biology
The Effects Of Ph On Chloride Flux In Fundulus Heteroclitus, Timothy Alton Washburn
The Effects Of Ph On Chloride Flux In Fundulus Heteroclitus, Timothy Alton Washburn
Master's Theses
The effects of increasing hydrogen ion concentration on rate of chloride influx and efflux were studied in the killifish, Fundulus heteroclitus, adapted to fresh water. Because increasing hydrogen ion concentrations have been shown to inhibit sodium influx and because evidence exists to support coupling of chloride transport with sodium transport, the present study was undertaken to determine what effect pH would have on rate of chloride flux. Chloride efflux rates were studied after the addition of sodium bicarbonate to the medium. No significant pH-dependent changes in efflux rate were observed, except at very low pH. Influx rates were significantly changed, …
Changes In The (Na+ K+) - Dependent Atpase Activity In Gills Of The Euryhaline Teleost, Fundulus Heteroclitus, Upon Adaptation To Freshwater And Salt Water As Determined By Specific Activities And 3h - Ouabain Binding Sites, Mary Elizabeth Gilman
Master's Theses
Previous attempts to measure the (Na++K+) - dependent ATPase in gills of euryhaline teleosts have yielded controversial results. In this study both the (Na++K+) - dependent ATPase activity and the number of JH - ouabain binding sites, representing the number of ATPase molecules, were measured in gill tissue preparations of the killifish, Fundulus heteroclitus. The (Na++K+) dependent ATPase activity and ouabain binding sites were greater in gills of fish fully adapted to freshwater than in those adapted to salinities of 17 ppt or 24 ppt. On the other …
Ddt Inhibition Of (Na+, K+)-Activated Atpase In Gill Tissue Of Fundulus Heteroclitus, Nancy Mccutcheon Slate
Ddt Inhibition Of (Na+, K+)-Activated Atpase In Gill Tissue Of Fundulus Heteroclitus, Nancy Mccutcheon Slate
Master's Theses
The DDT inhibition of (Na+, K+) - activated ATPase in gill preparations of the euryhaline teleost, Fundulus heteroclitus, was measured. The (Na+, K+) -activated ATPase was found to be sensitive to DDT, with 74.6% of the enzyme's specific activity inhibited by 20 ppm DDT. The (Mg++)-activated ATPase was also sensitive to inhibition bv DDT but not to the extent of the (Na+, K+) - activat ed ATPase . Due to previous work which showed that DDT antagonized valinomycin-induced K+ conductance in a synthetic membrane, the effect of K+ on DDT inhibition was examined kinetically. The Km for K+ in gill …
An Electrophoretic Comparison Of The Gill Plasma Membrane Proteins Of Freshwater And Saltwater-Adapted Killifish, Fundulus Heteroclitus, Earl Dean Hammit
An Electrophoretic Comparison Of The Gill Plasma Membrane Proteins Of Freshwater And Saltwater-Adapted Killifish, Fundulus Heteroclitus, Earl Dean Hammit
Master's Theses
A study was undertaken to compare proteins present in the gill membranes of freshwater and salt water adapted killifish, Fundulus heteroclitus. For this purpose, a method for the preparation or gill plasma membranes was developed. Polyacrylarnide gel electrophoresis indicated that the two types of gills possessed many membrane proteins in common. However, the freshwater adapted group possessed a single protein not found n the saltwater adapted group and the latter contained a protein not found in the freshwater adapted group.