Biology Commons^™

Study Of Plants Used Against Infections By California Native American Tribes, Maria J. Rojas

Master's Theses

The objectives of this research were to evaluate the antibacterial activity and to determine the chemical composition of a list of medicinal plants used by Native Americans in California. Artemisia californica, Mimulus aurantiacus, Equisetum telmateia, Equisetum hyemale, and Marah fabacea were selected from a list of plants reported as having been used for ailments related to infections by tribes located in California. The extracts obtained through steam distillation from E. telmateia, E. hyemale and M. fabacea were assayed for in vitro antibacterial activity against 16 Gram-negative and 6 Gram-positive bacteria using disk diffusion assays and measuring the diameters of inhibition …

Assessment Of Genetic Diversity In Largetooth Sawfish, Pristis Pristis, Populations Over The 20th Century, Ann Fearing

Master's Theses

The Critically Endangered Largetooth Sawfish, Pristis pristis, experienced global declines in range and abundance over the past century and Australia is now their last stronghold. This research aimed to understand whether these declines have been accompanied by a reduction in levels of genetic diversity. Using P. pristis tissue samples sourced from natural history specimens, three fragments (616-bp, 386-bp, 141-bp) in the mitochondrial DNA (mtDNA) control region were PCR-amplified. At each fragment, significant genetic structure was found in P. pristis overall (Φ_ST=0.946, N=9; 0.813, N=54; 0.771, N=99). The Indo-West Pacific and Atlantic/Eastern Pacific each harbor genetically distinct lineages of …

Sensory Stressors Impact Species Responses Across Local And Continental Scales, Ashley A. Wilson

Master's Theses

Pervasive growth in industrialization and advances in technology now exposes much of the world to anthropogenic night light and noise (ANLN), which pose a global environmental challenge in terrestrial environments. An estimated one-tenth of the planet’s land area experiences artificial light at night — and that rises to 23% if skyglow is included. Moreover, anthropogenic noise is associated with urban development and transportation networks, as the ecological impact of roads alone is estimated to affect one-fifth of the total land cover of the United States and is increasing in space and intensity. Existing research involving impacts of light or noise …

Transgenerational Plasticity Causes Differences In Uv-Tolerance Of Intertidal And Subtidal Populations Of The Purple Sea Urchin, Strongylocentrotus Purpuratus, Yareli Alvarez, Nikki L. Adams

Master's Theses

Planktonic larvae of marine organisms are increasingly being exposed and required to respond to a changing physical environment. Adult sea urchins occupy both intertidal and subtidal waters and broadcast spawn gametes into the water column to contend with variable physical conditions. To answer how populations of invertebrates residing at different depths adequately prepare their offspring to cope with different levels of ultraviolet radiation (UVR), we collected adult purple sea urchins, Strongylocentrotus purpuratus, from four sites (two intertidal and two subtidal (~15 m deep)) on the central coast of CA to compare UV tolerance in offspring. Our measurements of UVA …

Assessing Seasonal Changes In Body Condition For Spotted (Phoca Largha), Ringed (Pusa Hispida), And Bearded (Erignathus Barbatus) Seals, Michelle Hartwick

Master's Theses

Anthropogenic global warming is causing unprecedented changes to occur within Arctic ecosystems. As sea ice continues to decline in both thickness and extent, ice-adapted species will be particularly affected. Specifically, Arctic seals are reliant on sea ice during critical life-history stages and many of their preferred prey depend on reliable patterns in annual sea ice cover. Thus, ongoing changes will likely affect the ability of Arctic seals to carry out key life-history tasks and maintain positive energy balance. One way to assess energy reserves is through examination of body condition. For seals, this is often done by measuring overall blubber …

Feeling Out Your Food: A Histological Analysis Of The Whisker System In Seals, Cameron Cooper

Master's Theses

The vibrissal (whisker) system is present in nearly all mammals and is especially important in deep-diving mammals. Pinnipeds (seals, sea lions, and walruses) have highly sensitive whiskers that are used to follow hydrodynamic trails created by their swimming prey. Each pinniped vibrissa is surrounded by a tripartite blood sinus system composed of an upper cavernous sinus (UCS), a ring sinus (RS), and a lower cavernous sinus (LCS). The UCS has been hypothesized to play a thermoregulatory role, insulating temperature sensitive mechanoreceptors located within the vibrissal follicle. The aim of this study was to examine this hypothesis by measuring and comparing …

Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey

Master's Theses

Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti …

Pismo Clams (Tivela Stultorum) In Califorina: Population Status, Habitat Associations, Reproduction, And Growth, Alexandria R. Marquardt

Master's Theses

Marine shellfish play a vital role in intertidal ecosystems and coastal communities, but many of these fisheries are small-scale and lack the necessary monitoring to ensure long-term sustainability. Effective management often requires information on key demographic parameters, such as population status, reproduction and growth. Pismo clams (Tivela stultorum) are a culturally important and iconic species in California, which supported a thriving commercial and recreational fishery throughout much of the 1900’s. However, Pismo clam populations have declined statewide in recent decades and are attributed to human harvest and predation by California sea otters (Enhydra lutris); However, no …

Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Kathleen N. Ivey

Master's Theses

Recognizing how climate change will impact populations can aid in making decisions about approaches for conservation of endangered species. The Blunt-nosed Leopard Lizard (Gambelia sila) is a federally endangered species that, despite protection, remains in extremely arid, hot areas and may be at risk of extirpation due to climate change. We collected data on the field-active body temperatures, preferred body temperatures, and upper thermal tolerance of G. sila. We then described available thermal habitat using biophysical models, which allowed us to (1) describe patterns in lizard body temperatures, microhabitat temperatures, and lizard microhabitat use, (2) quantify the …

Investigating Dispersal Ability To Infer Diversification In The Birds Of Madagascar, Robert D. Lauer

Master's Theses

The objective of this study was to investigate whether dispersal ability, as measured by a proxy of hand-wing index 2, influenced diversification of the birds of Madagascar at two scales. Madagascar is home to several avian lineages that have diversified greatly while other lineages are only represented by a single species. a key question in evolutionary biology is why some of these lineages diversified while others did not. One way to address this is to examine what features of these lineages promotes their diversification. Recent studies have focused on the relative importance of dispersal ability to diversification at the continental …

Investigating The Nightime Departures Of Glaucous-Winged Gulls (Larus Glaucescens) And The Role Of Social Facilitation, Devon Leigh Mcclain

Master's Theses

Daytime behaviors and occupancy patterns of Glaucous-winged Gulls (Larus glaucescens) have been described and can be mathematically predicted based on environmental factors. However, little is known about the nighttime behaviors of Glaucous-winged Gulls. I used trail cameras to study the daytime and nighttime colony occupancy patterns of Glaucous-winged Gulls on a breeding colony on Protection Island, Washington, USA. Early in the breeding season gulls desert the colony en masse during nighttime even after some gulls have initiated clutches. Using acoustic recording units to identify an acoustic cue that signals the onset of the coordinated nightly departures from the colony, I …

Using Crispr-Cas9 As A Restriction Enzyme, Zack Crawley

Master's Theses

Restriction digests are a commonly utilized process for cleaving DNA at specific, but relatively common sites. Restriction enzymes have widespread use in DNA manipulation. CRISPR/Cas9 is a recently identified endonuclease which utilizes a customizable guide sequence to recognize and cut specific ~20 bp sites located in a DNA sequence. This preliminary research aimed to exploit the potential benefit of DNA restriction using the CRISPR/Cas9 procedure through alterations of different components involved in that system. We sought to refine existing CRISPR/Cas9 protocols and make a budget friendly, user-selectable CRISPR/Cas9 restriction digest protocol. The motivation for this research was to simplify and …

Paleoenvironmental Implications Of Small-Animal Fossils From The Black Mountain Turtle Layer And Associated Layers, Eocene Of Wyoming, Jeremy Mclarty

Master's Theses

The Bridger Formation is an Early Middle Eocene deposit in southwestern Wyoming that preserves a rich record of life from North America. Some horizons within the Bridger Formation contain abundant fossil turtle shells, but turtle skulls are rarely found. Previous research focused on one of these fossil-rich horizons, the Black Mountain turtle layer, to develop a model for the abundance and taphonomic condition of the fossil turtles. The proposed model begins with a limestone layer deposited in a lake. Then, an influx of fine-grained volcanic ash (forming the Black Mountain turtle layer) was rapidly deposited into this lake likely causing …

The Use Of Multiple Sensory Modalities By The Antillean Manatee (Trichechus Manatus Manatus) To Locate Food In Their Natural Environments, Amanda Marie Moore

Master's Theses

Manatees are herbivorous aquatic mammals found in the coastal and inland waters of the Atlantic Ocean. All three manatee species are currently listed as vulnerable on the IUCN red list and there still remains much unknown about their ecology. It is currently unknown what sensory modalities manatees use to locate their food in the wild. A literature review of the Paenungulata clade (sirenians, proboscideans, and hyracoideans) was conducted in order to compare and contrast what is known about the sensory modalities of the clade, to better understand the sensory modalities of manatees, particularly the ones they use to locate their …

Ground Beetle (Coleoptera: Carabidae) Assemblages In Native, Invasive, And Encroaching Grassland Habitats, Madison Pittenger

Master's Theses

Ground beetles (Carabidae) are increasingly used as ecological indicators in studies regarding land use because they are ubiquitous, respond quickly to environmental change, have a well-understood taxonomy, and can be trapped with ease. While the effects of various plant communities on ground beetle assemblages are relatively well-known, past studies have operated within boreal and tropical forests and have not placed much emphasis on the effects of native and nonnative species. In this study, ground beetles were investigated as indicators of invasion in a grassland setting. Ground beetles were sampled using pitfall traps throughout the 2018 growing season at Quivira National …

The Influence Of Land Use On The Pollen Diet Of Honey Bee (Apis Mellifera) Colonies In Ellis County, Kansas, Ryan Engel

Master's Theses

Pollinators are integral to plant ecology as well as to worldwide food availability and security. Understanding how human-driven land use change impacts the nutrition of managed honey bees is important in pollinator conservation efforts and can contribute towards combating recent pollinator declines. The objective of this study was to determine sources of forage for honey bees across different land use types in Ellis County, Kansas, through pollen analysis and taxonomic identification. Replicate study colonies were placed in three separate land use types: Urban, Cropland, or Native/Semi-native prairie. Pollen was sampled every 7 to 14 days throughout a growing season (April …

The Effects Of Prescribed Burning And Microhabitat Type On Ant (Formicidae) Functional Groups And A Survey Of Ants In The Dr. Howard Reynolds Nature Area (A Mixed Grass Prairie), Ashley N. Durr

Master's Theses

This project sought to understand how prescribed burning and microhabitat type impacts Kansas ant functional groups and also whether prescribed burning in different microhabitat types altered the burn’s impact on those functional groups. The Dr. Howard Reynolds Nature Trail, located in Hays, Kansas, was burned in the spring of 2019. The area consists of 2 distinct habitat types: a dry, mixed-grass dominated uphill area and a moist, densely vegetated downhill area. Pitfall trapping was conducted during the summers of the year prior to the burn (2018) and the year following the burn (2019). 15 pitfall traps were spread across each …

The Influence Of Landscape Factors On Black-Tailed Prairie Dog (Cynomys Ludovicianus) Colony Persistence In Northwest Kansas, Jamie Oriez

Master's Theses

The black-tailed prairie dog (Cynomys ludovicianus) is a colonial and fossorial rodent species that serves as an ecosystem engineer and keystone species in North America’s grasslands. Black-tailed prairie dogs historically ranged from northern Mexico to southern Canada, and from eastern Nebraska to the foothills of the Rocky Mountains. However, with the loss and fragmentation of grasslands, introduction of Sylvatic plague (Yersinia pestis), and control measures such as poisoning and shooting, black-tailed prairie dogs are limited to less than 5 percent of their historical range.

In this study, I examined how colony area, location, isolation, and surrounding …

A Study Of Inactive Enzyme-Homologues: The Biochemical And Biological Function Of Ecm14 In Saccharomyces Cerevisiae, Robert Christian Mcdonald

Master's Theses

Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains several pseudoenzymes predicted to lack enzyme activity and with unknown molecular function. The genome of the yeast Saccharomyces cerevisiae encodes only one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. Ecm14 is found throughout ascomycete fungi, with a group of related pseudoenzymes found in basidiomycetes. Although the prodomain of Ecm14 can be cleaved in vivo and in vitro by endopeptidases, suggesting an activation mechanism, no activity has been detected using standard carboxypeptidase substrates.

In order to examine the function …

Exploring The Biological Function Of Carboxypeptidase O: Analysis Of Expression And Association With Chylomicrons And Lipid Droplets, Erika Bauza Nowotny

Master's Theses

Carboxypeptidase O (CPO) is a membrane-bound peptidase that cleaves acidic and polar C-terminal amino acids of peptides; however, its biological function remains unknown. CPO is strongly expressed in the small intestine, where it has been proposed to participate in digestion events at the brush border by extracellular cleavage of dietary peptides. At the subcellular level, CPO is anchored to the inner leaflet of the endoplasmic reticulum (ER) membrane, where it has been shown to associate with lipid droplets (LDs). The ER membrane also represents the site of iv chylomicron formation, a process that shares many similarities with that of LDs …