Biology Commons^™

Determining Parentage And The Effects Of Relatedness On Play Partner Preference In Belding's Ground Squirrels, Jessica Weidenbach

Master's Theses

I investigated 1) multiple paternity in Belding’s ground squirrels (Urocitellus beldingi) and 2) social partner preferences in juvenile U. beldingi. Prior work with blood allozymes demonstrated multiple paternity in U. beldingi litters. I evaluated paternity using microsatellite DNA analysis, which is more accurate than examining blood allozymes. My results indicate that multiple paternity in U. beldingi is more extensive than previously shown, occurring in about 90% of litters with more than one juvenile, and averaging 2.95 fathers in multiply sired litters. I also evaluated the hypothesis that play and other social behavior promotes bonding among juvenile female …

Protective Effects Of Sphingomyelin Against Uv Photodamage In Human Keratinocytes, Kathleen De Guzman

Master's Theses

Ultraviolet (UV) radiation has been demonstrated in numerous studies to be a major risk factor for non-melanoma skin cancer development. Despite the emergence of current UV-preventative strategies, such as sunscreens and skin-protective clothing, the incidence of non-melanoma skin cancer has continued to rise. This has encouraged investigations on alternative methods for UV prevention. In particular, bovine milk sphingomyelin has been studied for its potential in protecting human skin against UV photodamage. While the previous studies have suggested that sphingomyelin exhibits UV-protective properties in a human skin equivalent model, the exact mechanisms behind sphingomyelin’s photoprotective effects are yet unknown.

This thesis …

The Influence Of Hydrogeomorphology, Soil Redox Conditions, And Salinity On The Spatial Zoning Of Saltgrass, Salt Rush, And Cattails In Scotts Creek Marsh, Swanton Pacific Ranch, Ca, Mark D. Gormley

Master's Theses

Scotts Creek Marsh (SCM) is a small coastal wetland ecosystem in Davenport, CA. The vegetation of SCM is dominated by three halophytic zones comprised of saltgrass, salt rush, cattails. The objectives of the study were (i) to investigate the variables that influence the zoning of the three dominant halophyte communities in SCM and (ii) to the test the effectiveness of Indicator of Reduction in Soil (IRIS) tubes to indicate the reduction of S. The study examined the following parameters from April 6 to July 21, 2013: (i) the HGM of Scotts Creek Marsh, (ii) soil oxidation and reduction (redox) conditions, …

The Behavioral And Physiological Effects Of Long-Distance Translocation On Western Rattlesnakes (Crotalus Oreganus), Kory Hayden Heiken

Master's Theses

Long-distance translocation (LDT), the relocation of an animal outside of its home range, is a popular strategy for mitigating conflict between humans and venomous snakes. While LDT has been demonstrated to prevent a snake’s return to the location of capture, it may result in increased mortality, magnitude and frequency of movements, and activity range sizes. Thus, it has generally been discouraged. However, the effects of LDT on stress physiology and thermoregulation have gone largely unstudied in reptiles. To elucidate these effects, we conducted an experimental LDT on Western Rattlesnakes (Crotalus oreganus) on Vandenberg Air Force base in California. …

Lipid Production By Microalgae Treating Municipal Wastewater, James Edward Kelley

Master's Theses

Microalgae hold much promise as a feedstock in liquid biofuel production. Lipid content of microalgae cells range from 30-80% dry weight of biomass. It is projected that microalgae can produce between 1,000-6,500 gallons/acre/year of oil. Currently, production of industrial algae operates in open raceway ponds that use minimal capital and energy inputs to culture algae. Raceway ponds can also be used to grow microalgae from municipal waste streams. Although high biomass productivity can be achieved in these systems, there remains a large production gap between large volumes of biomass cultivation and high lipid content from microalgae cells. Low lipid content …

An Insight Into The Microbial Diversity And Expression Of Cysteine Protease Inhibitors (Cystatin) In Rickettsia Parkeri Infected Amblyomma Maculatum, Khemraj Budachetri

Master's Theses

Amblyomma maculatum (Gulf Coast tick) is an emerging tick species of public health significance in United States. It is a competent vector of Rickettsia parkeri, an etiological agent of a human rickettsiosis. In this study, we investigated the spotted fever group of rickettsial diversity in A. maculatum based on rickettsial ompA gene PCR. Our results showed A. maculatum harbors R. parkeri, R. amblyommii, and R. endosymbiont of A. maculatum. While only R. parkeri was detected in female salivary glands which suggest its ability to traffic from midgut to salivary glands via hemocoel. The presence of R. parkeri was further …

Comparative Analysis Of Antiviral Responses In Mouse Embryonic Stem Cells And In Mesenchymal Stem Cells, Jundi Wang

Master's Theses

Embryonic stem cells (ESCs) are cells that have unlimited capacity for selfrenewal and differentiation. These properties make ESCs a great cell source for application in regenerative medicine. When used for cell therapy, ESC-derived cells could be placed in a wounded area that is likely to be exposed to various pathogens. However, it is not well-understood whether ESCs and ESC-derived cells have active antiviral responses against infectious agents from the environment. To answer this important question, I comparatively analyzed the antiviral responses of ESCs and mesenchymal stem cells (MSCs, C3H10Tl/2 cell line) to infectious agents. Using the expression of type I …

Root Kinematics In Relation To Temperature And Genome Size In Wild And Domesticated Zea., Avery B. Cromwell

Master's Theses

We studied root kinematics in relation to temperature and genome size variation in teosinte (Zea mays subspecies parviglumis) and corn (Zea mays subspecies mays). Corn had significantly faster radicle growth than teosinte when grown at a constant temperature. Both species exhibited variation in seed size and for each species larger seeds had faster root growth. Genome size was not significantly correlated with faster radicle growth rates across multiple land races of corn. To examine temperature dependent growth in corn and teosinte, a germinated seedling was grown at multiple temperatures. Growth rates at these temperatures were used …

Testing Subspecies Limits In Monardella Villosa, Taylor Mclaughlin Crow

Master's Theses

Several subspecies of Monardella villosa (California Coyote Mint) have been distinguished on the basis of leaf thickness, shape, and trichome characteristics, yet many intermediates are known. We investigated morphological differences in natural populations of two subspecies (M. v. subsp. villosa and M. v. subsp. franciscana) in the Scott Creek watershed, north of Davenport, in Santa Cruz County, CA. Monardella villosa subsp. franciscana grows in coastal scrub in gulches and ocean terraces whereas subsp. villosa grows at more protected inland sites. Morphological difference between subspecies may be adaptations to these different habitats. I grew plants in a common garden at Cal …

Associations Between Alcohol Consumption And Fasting Blood Glucose In Young Adults, Julie Ann Lucca

Master's Theses

Current research shows moderate alcohol consumption is associated with decreased risk of diabetes and excessive consumption or binge drinking can cause insulin resistance and diabetes. In 2010, diabetes was the seventh leading cause of death in the United Statesand was responsible for significant health complications: blindness, kidney failure, and limb amputations, and is a large national economic burden. Fasting blood glucose (FBG) is a tool used to help diagnose diabetes. Abnormally high FBG, ≥100 mg/dl, is indicative of diabetes and pre-diabetes. Few studies have observed diabetic prevalence among young adults or college students. Studying young adults can help provide added …

Influence Of Wind Turbines On Mammalian Occupancy Patterns, Brian P. Tanis

Master's Theses

Wind energy is among the most rapidly growing energy industries in the United States, with support for development coming from both state and federal governments. While the industry depicts an environmentally friendly image, the addition of infrastructure associated with wind farms alters landscapes in novel ways. Numerous studies have documented impacts wind turbines have on bird and bat mortality; however, far less attention has been directed towards responses of non-volant, terrestrial organisms. Mammalian mesocarnivores are model organisms to assess the alteration of communities surrounding wind turbines as they respond to addition of turbines and human activity, addition and improvement of …

Effects Of Long-Term Exposure To Kanamycin And/Or Ampicillin On Resistance Genes On An E. Coli Plasmid, Justin W.R. Kerby Ii

Master's Theses

Multi-drug-resistant bacteria are a major cause of hospital-acquired infections and antibiotic resistance in these organisms is often plasmid mediated, which has become a growing concern. Plasmids conferring resistance to multiple antibiotics are increasingly becoming a common source of antibiotic resistance. The behavior of these plasmids under and in the absence of selective pressure is not yet fully understood. Therefore, to determine the behavior of a multiple-resistance plasmid under selective pressure pCR 2.1-TOPO, a commercial plasmid, was inserted into an Escherichia coli host and grown in a continuous culture under four conditions: broth with 1) kanamycin alone, 2) ampicillin alone, 3) …

Stable Isotope Analysis Of Two Mephitidae Species Reflects Population Trends And Landscape Structure, Amanda E. Cheeseman

Master's Theses

The eastern spotted skunk (Spilogale putorius) is a small omnivorous Carnivora similar in much of its natural history to the commonly found striped skunk (Mephitis mephitis). Spilogale putorius has experienced drastic population declines over a large portion of its geographic range. Many hypotheses for the decline of S. putorius have been proposed. δ13C and δ15N isotope analysis provides a unique opportunity to examine diet over an expanded time span. Thus was used on hair sampled from natural history collections, teaching collections, and road kills to examine dietary change for both S. putorius and M. mephitis from 1852 to 2012. Because …

Antimicrobial Producing Bacteria Isolated From Petroleum-Laced Hypersaline Soil, Joanna L. Fay

Master's Theses

Given the alternative functions of “antibiotics” as communication molecules or participants in metabolism, it seems probable that production might be influenced by factors such as nutrient availability, interactions with neighboring microbes, and/or colony or community structure and maturity. With this in mind, the present study aimed to broaden the scope of the search for novel antibiotics by experimenting with the following parameters: source of bacterial isolation, growth and assay media, and culturing techniques. Bacteria for this study were isolated from two categories of soil (petroleum-contaminated or uncontaminated) to compare diversity and antimicrobial activity. Compared to the uncontaminated soil, isolates of …

Interactions Between Protozoan Prey (Phylum Protista) And Their Mosquito Predators (Order Diptera, Family Cuclicidae) Prey Size And Predator Behavior Effects, Jeffrey Jay Skiff

Master's Theses

Mosquitoes (Diptera. Culicidae) are insects that are medically important as adults as they vector numerous diseases. Yet, understanding the ecology of the larval stage can lead to surveillance and control of adult populations. Aedes albopictus, Aedes triseriatus, Cu/ex corona/or, and Culex quinquefasciatus are four species of mosquito that co-occur in discarded automobile tires. Mosqui-to larvae feed on microorganisms (including protozoans) and particulate organic matter Most larval feeding experiments. howt:ver, have dealt exclusively with bacteria, fungi , and particulate organic matter Although bactena and fungi are important sources of nitrogen and lipids, they may be 111sufficient sources of carbon, which may …

Home Range And Habitat Use Of Santa Rosa Island Foxes (Urocyon Littoralis Santarosae), Elizabeth Marie Drake

Master's Theses

Island foxes (Urocyon littoralis) are currently listed as federally endangered on four of the six Channel Islands to which they are endemic. The Santa Rosa Island (SRI) population declined by 99% during the 1990’s due to non-native golden eagle (Aguila chrysaetos) predation and is currently the lowest fox population (~280) and density (0.86 foxes/km²) of any of the Channel Islands. The goals of this study were to assess new miniaturized GPS technology and to quantify home range and habitat use of the SRI population. This is only the second use of Global Positioning System (GPS) …

Behavior And Ecosystem Effects Of The Invasive Asian Clam (Corbicula Fluminea) In Urban Streams, Kayla Turek

Master's Theses

Invasive species can be detrimental to freshwater ecosystems. By completing laboratory and field studies to observe processes and behaviors of the invasive Asian Clam (Corbicula fluminea), I documented pathways whereby this invasive species impacts aquatic ecosystems under conditions typical of urbanized streams I found. The predominant pathways by which clams impacted nitrogen (N) cycling was through excretion thus increasing ammonium (NH4+) flux out of sediment and through bioturbation, which increased nitrate (NO3-) diffusion to the sediment and dinitrogen gas (N2) production (i.e., denitrification). The effect was greater under urban conditions, where C. fluminea population density and water column NO3- were …