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The Induced Differentiation Of Mouse Ectoderm To Epidermis By Growth On Freeze-Thawed Dermis, Albert Joseph Banes
The Induced Differentiation Of Mouse Ectoderm To Epidermis By Growth On Freeze-Thawed Dermis, Albert Joseph Banes
Master's Theses
Previous investigators have grown or maintained adult or embryonic skin in tissue culture and on the chorioallantoic membrane (CAM) of chickens. These studies have revealed two important factors involved in culturing skin: (1) the presence of an inducer which originates in the dermis and affects the phenotype of epithelial cells; and (2) the influence of the physical surface of the substrate on which epidermis is cultured.
The current investigation presents evidence that ectoderm from the limb buds of 10 to 12 day-old mouse embryos can form an epidermis when combined with dermis killed by alternate freezing and thawing, and cultured …