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Master's Theses

University of Richmond

Blue crab

Publication Year

Articles 1 - 2 of 2

Full-Text Articles in Biology

Atp Defendent Na+ Transport And The Characterization Of Na+ / Nh4+ Exchange In Basolateral Vesicles Of The Blue Crab, Callinectes Sapidus, Gill, Linda Ann Fuhrman Aug 1985

Atp Defendent Na+ Transport And The Characterization Of Na+ / Nh4+ Exchange In Basolateral Vesicles Of The Blue Crab, Callinectes Sapidus, Gill, Linda Ann Fuhrman

Master's Theses

ATP dependent Na+ transport was denonstrated in purified ba.solateral vesicles of the blue crab (Callinectes sapidus) gill. Active Na+ uptake was dependent upon the intravesicular presence of either K+ or NH; as a counterion. Final membrane preparations consist of inside-out basolateral vesicles, evidenced by intravesicular ouabain (1 rrM) and extravesicular vanadate (1 rnM) 22Na + uptake inhibition. Na+/NH4+exchange rates were investigated by detennining the NH; concentration in the recovered extravesicular nedium and by using the kinetic capabilities of a DU7 spectrophotareter. These purified basolateral membranes, rich in Na+,K+ …


The Role Of An Eyestalk Extract In The Regulation Of The Blood Sodium And Calcium Concentrations Of The Blue Crab Callinectes Sapidus, Gregory Martin Ivy May 1976

The Role Of An Eyestalk Extract In The Regulation Of The Blood Sodium And Calcium Concentrations Of The Blue Crab Callinectes Sapidus, Gregory Martin Ivy

Master's Theses

The effect of bilateral eyestalk ligation or ablation on the blood Na+ and ca++ concentrations of the euryhaline blue crab, Callinectes sapidus, was measured in 10 o/oo and 5 o/oo sea water. The ablations caused a significant decrease in the blood Na+ and ca++ over a 24 hour period. The serum Na+ level increased significantly 6 hours following injections of an eyestalk extract whereas the blood total ca++ level did not change significantly. Eyestalk ligation, however, did not cause significant blood Na+ and ca++ changes. The blood Na+ level of intact crabs increased significantly in acclimating to colder water when …