Biology Commons^™

Intestinal Absorption And Lipolysis Of Safflower Oil And Other Unsaturated Vegetable Oils In Rats, Robert Leslie Gregory

Master's Theses

The intestinal lipolysis and absorption of safflower, corn, peanut, olive, cottonseed and soybean oils were studied in the rat. Oils and pancreatic lipase were injected into the rat jejunum and ileum (ligated in situ), and the amount of esterified fatty acids absorbed and free fatty acids present in the gut after 3 hours was determined.

Safflower oil was absorbed significantly less than the other oils. There was no significant difference between the absorption rates of the other oils. When the oils were subjected to porcine pancreatic lipase in vitro, safflower oil also exhibited the lowest rate of hydrolysis.

The rates …

The Induced Differentiation Of Mouse Ectoderm To Epidermis By Growth On Freeze-Thawed Dermis, Albert Joseph Banes

Master's Theses

Previous investigators have grown or maintained adult or embryonic skin in tissue culture and on the chorioallantoic membrane (CAM) of chickens. These studies have revealed two important factors involved in culturing skin: (1) the presence of an inducer which originates in the dermis and affects the phenotype of epithelial cells; and (2) the influence of the physical surface of the substrate on which epidermis is cultured.

The current investigation presents evidence that ectoderm from the limb buds of 10 to 12 day-old mouse embryos can form an epidermis when combined with dermis killed by alternate freezing and thawing, and cultured …

A Comparative Study Of Solubilized Proteins From Phycomyces Blakesleeannus By Disc Electrophoresis, Douglas Burton Gillespie

Master's Theses

Mycelial, sporangiophore, and zygospore solubilized protein fractions o Phycomyces blakesleeanus Burgeff were separated on polyacrylamide gels and stained. Protein patterns of 8 and 13 day old mycelia of the + strain were identical as were the patterns of the same two age groups of the - strain. The + mycelia exhibited 9 protein bands while the - strain contained 10 bands. Seven bands were homologous to both strains. Sporangio­phore samples of 5 and 10 day old cultures from + and - strains contained 4 protein bands homologous to all 4 samples. The protein band at 9 mm was also …

A Determination By Thin-Layer Chromatography Of The Chemical Nature Of The Poison Produced By The Bryozoan Lophopodella Carteri (Hyatt), John Michael Dolan

Master's Theses

A study was undertaken to isolate and characterize by thin-layer chromatography the poisonous principle produced by the freshwater bryozoan, Lophopodella carteri (Hyatt).

The bryozoan homogenate was divided into a lipid and non-lipid portion and each was assayed for toxicity. Only the lipid extract was lethal to fishes. The lipid part was divided into six classes by thin-layer chromatography. When tested, four of the six classes were toxic. Thin­ layer chroatography was used to subdivide each class into fractions. Assays showed toxicity in nine of fourteen fractions.

Evidence indicates that the poisonous princi ple is a lipid and the large number …