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Dissolved Organic Matter Influences The Timing Of Embryonic Development Of The Purple Sea Urchin, Strongylocentrotus Purpuratus., Corbin J. Hodges
Dissolved Organic Matter Influences The Timing Of Embryonic Development Of The Purple Sea Urchin, Strongylocentrotus Purpuratus., Corbin J. Hodges
Master's Theses
Marine dissolved organic matter (DOM) comprises one of the largest carbon reservoirs on earth and has long been considered a potential energy source for marine invertebrates. The importance of DOM transport has been adequately demonstrated for unicellular organisms, where DOM can meet 100% of an organisms energy needs, but the effects of DOM uptake for marine metazoans are less well understood. In this study, three general areas involving the influence of DOM transport to marine invertebrates were explored. First, we assessed the effects of using seawater exposed to high intensity ultraviolet radiation (UVR) on the study organism; embryos of the …
Exposure To Ultraviolet Radiation Causes Proteomic Changes In Embryos Of The Purple Sea Urchin, Strongylocentrotus Purpuratus, Joseph Paul Campanale
Exposure To Ultraviolet Radiation Causes Proteomic Changes In Embryos Of The Purple Sea Urchin, Strongylocentrotus Purpuratus, Joseph Paul Campanale
Master's Theses
The amount of solar ultraviolet radiation (UVR, 290-400 nm) reaching Earth’s surface is increasing due to ozone depletion and global climate change. Embryos of the purple sea urchin, Strongylocentrotus purpuratus, provide an ideal system for examining how UVR affects developing marine organisms and cells in general. To model the protein-mediated cell cycle response to UV-irradiation, six batches of S. purpuratus embryos were exposed to UVR, monitored for delays in the first mitotic division and examined for global proteomic changes. Embryos from each batch were exposed to or protected from artificial UVR for 25 or 60 min. Embryos treated with …