Biology Commons^™

Collective Prey Capture By Juvenile Venus Flytraps (Dionaea Muscipula), Steven Rosen

Honors Theses

The Venus flytrap (Dionaea muscipula), first described by Charles Darwin, is a unique carnivorous plant which acquires prey using leaf blades modified into snap-traps. A recent study showed that prey capture by flytraps was not selective. With this study we examined prey capture rates further by analyzing D. muscipula traps less than 1 em in length to determine if juvenile flytraps differ from adults. Similar to the earlier findings, trap size appeared to play no role in prey capture success. This was likely due to the fact that 88% of all prey items recovered were smaller than the smallest collected …

Is The Zebrafish Zombie Mutant Caused By A Mutation In Cdc20?, Peyton Johnston

Honors Theses

The zombie mutant was identified as an early arrest mutant, stopping in development around the 10-somite stage (14 hours of development). Further inspection revealed that this mutant was a cell cycle mutant and cells in the mutant arrest during metaphase as early as the 5-somite stage (11.6 hours of development). A similar phenotype is seen in the Drosophila melanogaster cell cycle gene fizzy, known to be to be a homolog of the Saccharomyces cerevisiae gene, cell division cycle 20 (cdc20). CDC20 is an activator protein of the anaphase promoting complex/cyclosome (APC/C), an ubiquitin E3 ligase that is …

Exploration Of The Range Overlap Between Cynomys Leucurus And Cynomys Ludovicianus, Kenneth D. Choi

Honors Theses

The ranges of two species of prairie dogs, Cynomys leucurus and Cynomys ludovicianus, overlap in a narrow area in the western Great Plains in Wyoming. We used GIS software to map their distributions and infer land cover preferences within and on either side of the zone of overlap. In general, both species used habitats in proportion to their availability and did not display strong selection of their expected vegetation types (based on the literature). However, both species of prairie dogs were overrepresented in areas of human settlements and crops, which may indicate that these species prefer human-modified habitats, or perhaps …

L3 Auditory Interneuron Responses To Dual-Frequency Calling Songs In Female Crickets (Acheta Domesticus), Jeong Bin Lee

Honors Theses

Female crickets exhibit positive phonotaxis in response to calling songs with specific carrier frequencies (4-5 kHz), syllable periods (SP; 50-70ms), and other parameters. Among a number of ascending prothoracic auditory neurons identified, only L3 has been proposed to be involved in SP selective phonotaxis. L3 responds with selective decrement to 5 kHz stimulus with attractive SPs. Furthermore, L3 is highly tuned to 16 kHz auditory stimulus, which has been correlated with aversive phonotactic behaviors. L3’s cellular responses to simultaneous 16 kHz and 5 kHz stimuli were examined through extracellular recording, thereby further establishing the connection between L3’s selective decrement and …

A Comparison Of Gastrointestinal Bacterial Population Between Indoor Cats And Outdoor Cats, Farhana Binti Ikmal Hisham

Honors Theses

This study used fecal samples from four indoor cats and four indoor-outdoor cats in order to identify the microbial communities in the gut of cats. This information was then used to compare the microbial phyla between both groups for any differences. Total microbial DNA was isolated from each fecal sample, and the 16S rRNA gene was sequenced using Illumina MiSeq high throughput method. The sequences were identified using the bioinformatics program mothur. The results show that indoor cats had a more diverse microbial community as compared to outdoor cats. Indoor cat samples had 26% more microbial species, and eight more …

Does Gut Microbiota Affect The Diet Preference In Anurans?, Jordan A. Rice

Honors Theses

Gut microbiota is a community of bacteria that live in the digestive track of a host. These microbes assist in the breakdown of indigestible materials as food passes through the alimentary canal. Metabolites from bacteria may play a role cell to cell communication with their host and thus gut microbiota may affect the diet preference of the animal host. Southern Leopard frogs (Rana sphenocephala) and Green Tree frogs (Hyla cinerea) were used as focal species to test whether gut microbiota affect their diet preference. Three groups of tadpoles were tested. The control group was fed a …

The Effect Of Dietary Salt Intake On The Subcellular Distribution Of Epithelial Na+ Channels In Vasopressin And Oxytocin Synthesizing Neurons In The Rat Hypothalamus, Richard Frank Guidry

Honors Theses

No abstract provided.

Anticancer Activity Of Some Boronic Acid Arylidene Heterocycles, Joy Kim

Honors Theses

This research was done in order to investigate the anticancer activity of boronic acid arylidene heterocycles. Boronic acid, heterocycles, and aryl compounds have all independently exhibited various anti-microbial properties and have shown potential to be used in pharmaceuticals for the treatment of viruses and cancers. Using a one-step protocol developed by Dr. Murray, several different arylheterocyclic compounds were combined with an arylboronic acid to synthesize novel compounds. These were then tested for their anti-cancer activity on glioblastoma brain cancer cells. Although varied in potency, all had some amount of anti-cellular effect on the cancer cells. This is only the start …

Lack Of Glucocorticoid Receptor Hypersensitivity-Related Polymorphisms In An Undergraduate Population, Evan Leibovitz

Honors Theses

Obesity is a currently a major problem plaguing the world as a whole. The International Obese Task Force (IOTF) estimates at least 1.1 billion adults are overweight with some 312 million of those obese, globally. 1 As of 2004, in less than twenty years, the prevalence of obesity had more than doubled in adults with childhood obesity prevalence rising even more rapidly. Obesity, however, can be classified as a disease of its own or as a symptom of another disease. Two such diseases that have obesity as a symptom are Metabolic Syndrome and Cushing ’s syndrome. Metabolic Syndrome is a …

The Effects Of Canopy Structure On Photosynthesis In Sphagnum Mosses, Eric Matthew Ward

Honors Theses

In open habitats, Sphagnum grows with a dense architecture to retain water; mosses adapted to survive in shade persist in looser canopies. It is unknown if there is a physiological advantage of such rough canopies in the shade, but they may have enhanced photosynthesis at low light angles as would occur during spring or fall. It was hypothesized that the rough canopies would have enhanced rates of photosynthesis at lower angles. To test this, a photosynthesis system interfaced with a custom chamber was used to generate light response curves. Preliminary results of the study found no difference in photosynthesis with …

Flow Cytometry And Biochemical Analysis Of Apoptotic Mouse Ht-2 T - Lymphocytes, Nell Pinkston

Honors Theses

Apoptosis is a highly organized intracellular death program in multicellular animals. In the vertebrate immune system, apoptosis plays a central role in preventing the emergence of autoreactive lymphocytes. In this study, we used multiple stimuli (staurosporine, camptothecin, and cytokine deprivation - interleukin-2 or IL-2) to initiate apoptosis in IL-2 dependent, mouse HT-2 T-lymphocytes. All three inducers triggered DNA laddering and phosphatidylserine externalization. Propidium iodide staining and flow cytometry were also used to determine whether apoptotic cells accumulated in a specific stage of the cell cycle, and whether the mode of induction affected cell cycle distribution. Our findings indicate that IL-2 …

The Influence Of Polyamines On The Hypotonic Shock Response In The Euryhaline Teleost, Fundulus Grandis, Keith Lasko

Honors Theses

No abstract provided.

Isolation And Characterization Of A Novel Extremely Halophilic Euryarchaeota From Hypersaline Cinders And Sediment, Shannon Mcduff

Honors Theses

No abstract provided.

Employing Extracellular Vesicles To Overcome Immunological Barriers To Aav- Mediated Gene Transfer & Ethical And Policy Implications Of Orphan Drug Development And Accessibility, Zachary Fitzpatrick

Honors Theses

No abstract provided.

Studies To Elucidate The Location And Function Of Stat5a/Pyruvate Dehydrogenase Complex In Adipocytes, Hardy Hang

Honors Theses

No abstract provided.

Alternative Material Nest Boxes And Impacts On Nestling Physiology And Adult Behavior In The Eastern Bluebird (Sialia Sialis), Jamie E. Jackson

Honors Theses

In the mid-nineteenth century, Eastern Bluebird (Sialia sialis, a native cavity nesting species) populations experienced serious declines because of a decrease in natural cavities and the introduction of non-native, competitive cavity nesting species. The creation of nest box programs led to an increase in bluebird populations and these programs continue to be of importance. In this study, a recycled metal ammunition can was used as a nest box to determine if this was a viable resource for bluebirds. This work investigated the effects of this alternate material nest box on nestling physiology and adult behavior with special emphasis …

Family Planning, Pregnancy, And Birth In Guatemala: Maya Women And Modern Healthcare, Kelsay Payton

Honors Theses

Within Maya populations in rural Guatemala, childbearing is considered a sacred and spiritual experience, in addition to a physical one. This country is home to some of the highest rates of maternal and infant mortality in Latin America, along with the largest disparities in health care, primarily between the indigenous Maya and ladino women (Schooley 2009). The use of biomedical reproductive services remains surprisingly low among the Maya, while it is continually rising in the ladino populations. To increase the overall reproductive health care in these indigenous communities, attention must be given to the understanding of Maya’s beliefs, therefore enabling …

Ph Dependence Of Dna Binding Of Dna Polymerase 1klenow-Like Fragments From D. Radiodurans, T. Aquaticus,And .E Coli, Katelyn Jackson

Honors Theses

No abstract provided.

Shell Matrix Protein Gene Composition In Two Marine Bivalve Species, Saxidomus Gigantea And Mytilus Trossulus., Katie Benson

Honors Theses

No abstract provided.

Crematogaster Pilosa Ants As Bioindicators Of Ecosystem Marsh Health, Elliott R. Thompson

Honors Theses

No abstract provided.

Investigation Of The Genetic Interactions Between Hippo Signaling Pathway And Drosophila C-Terminal Src Kinase (Dcsk), Hailey Jimin Kwon

Honors Theses

The focus of my research revolved around the intersection between the Hippo tumor suppressor pathway and the Src oncogenic pathway. Both pathways control tissue and organ size during development by regulating cell proliferation, cell death, cell migration, and cell adhesion. Aberrant functions in either pathway are often detected in human cancers and correlate with poor prognosis. The Drosophila C-terminal Src kinase (dCsk) is a genetic modifier of warts (wts), a tumor-suppressor gene in the Hippo pathway, and interacts with the Src oncogene. Reduction in dCsk expression and the consequent activation of Src are reported in hepatocellular and colorectal tumors. …

Coding Dna Into Music: An Alternate Way Of Analysis, Samuel Fesenmeier

Honors Theses

In the study, we are analyzing the human genome in order to determine patterns that may tell something about how DNA functions. Patterns require an explanation: it is highly improbable that they are random occurrence. These patterns may hint to something about how DNA functions. There are known patterns already discovered in DNA. For example, in the coding portion, three base pairs translate to a specific amino acid. In the non coding portion, however, specific patterns are not as simple.

We will search for patterns by applying a coding system that turns DNA into music. Music may serve as a …

His-Tagged And Myc-Tagged Βca Fusion Genes May Aid In The Characterization Of The Role Of Carbonic Anhydrases In Photosynthesis, Dequantarius Speed

Honors Theses

No abstract provided.

Characterization Of A Novel Retrotransposon In New World Monkeys, Sreeja Sanampudi

Honors Theses

No abstract provided.

Identification Of A New Fatty Acid Catabolic Pathway During Heterotrophic Growth In Chlamydomonas Reinhardtii, Clayton Mccuiston

Honors Theses

No abstract provided.

Understanding A Possible Wonder Drug: A Radial Diffusion Assay For The Rapid Evaluation Of Antimicrobial Peptides, Dustin Walter

Honors Theses

Antimicrobial peptides (AMPs) have been a major research focus due to their potential to combat a variety of human pathogens. Our laboratory has identified several novel peptides that display significant antifungal activity. The effectiveness of these peptides in vitro has been promising; however, it has been shown that physiological concentrations of various salts along with other conditions are inhibitory to peptide activity. To further explore the inhibitory effects of these salts, a new assay was developed whereby we can observe the effects of various salts on the peptide killing activity. For our studies, we employed several clinical isolates of Candida …

Mobilization Of Alu Elements Throughout Papio And Theropithecus Genomes, Emily Bennett

Honors Theses

No abstract provided.

Effects Of Soil Environment On Abundance, Diversity And Community Structure Of Ectomycorrhizal Fungi In Pinus Radiata, Sarah C. Steele

Honors Theses

Balanced and sustained nutrient cycles are critical to the success of the world's forest ecosystems. Research has shown ectomycorrhizal fungi (ECM) play a vital role in regulating these systems, as they store and mobilize cycled nutrients for the trees they colonize. Not all species of ECM allocate essential nutrients (nitrogen, phosphorous and carbon) to their host with the same efficiency. Studies have shown the soil environment is important for determining fungal distribution. To better understand the ECM fungal community of Pinus radiata, I explored the effects of soil characteristics (percent silt, percent clay, percent sand and soil water content) on …

Does This Have Gluten? Comparison Of Gluten-Free And Unrestricted Diets In Intestinal Bacterial Populations And Diversity, Bradshaw Hammond

Honors Theses

As different diets continue to grow in popularity within the United States, the effect of these diets upon bacteria in the intestines is only beginning to be understood. This study focused on the effect that a shift to a gluten-free diet has on the human gut microbiome. Three genetically close subjects were selected for observation over a six month time period. One subject observed a consistent gluten-free diet. A second subject started on an unrestrictive diet, switched to a gluten-free diet for three months, then returned to an unrestricted diet for an additional three months. The third subject left an …

Spatial Variation Of Bacterial Communities On The Leaves Of Southern Magnolia Trees, Heather L. Valdin

Honors Theses

Bacteria are found in almost all environments on earth, including on the surface of other living things. Bacteria that exist on the leaves of plants (the phyllosphere) are of interest because of the potentially harsh nature of the environment they inhabit, and of applied interest. Although there has been a substantial amount of research in phyllosphere microbiology, few studies have examined the effect of spatial variation on phyllosphere community composition. This project investigated the effect of spatial variation on the bacterial communities living on the surface of Magnolia grandiflora leaves. Following DNA extraction, next generation sequencing of 16S rRNA gene …