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Phorbol Ester Activation Of An Nhe-Like Electroneutral Na+ /H+ Antiporter In Isolated E-Cells Of Lobster (Homarus Americanus) Hepatopancreas, Jeff Duerr, Gregory A. Ahearn
Phorbol Ester Activation Of An Nhe-Like Electroneutral Na+ /H+ Antiporter In Isolated E-Cells Of Lobster (Homarus Americanus) Hepatopancreas, Jeff Duerr, Gregory A. Ahearn
Faculty Publications - Department of Biological & Molecular Science
The basolateral membrane of Atlantic lobster (Homarus americanus) epithelium possesses an electroneutral Na+ /H+ antiporter that functionally resembles members of the vertebrate NHE family. Regulatory mechanisms of this antiporter in purified hepatopancreatic E-cell suspensions, produced with a centrifugal elutriation technique, were investigated. Suspensions routinely consisted of greater than 95% E-cells displaying greater than 90% viability. Intracellular pH (pHi) was monitored by loading cells with the fluorescent dye 2´,7´-bis(carboxyethyl)-5,6- carboxyfluorescein (BCECF), and placing suspensions in a spectrofluorometer. Recovery from induced acid-loading was mediated by a Na+ -dependent, dimethylamiloride-sensitive proton efflux. Antiport activation was a sigmoidal function of pHi at values below …
Radiomorphometry And Biomechanical Assessment Of Recombinant Human Bone Morphogenetic Protein 2 And Polymer In Rabbit Radius Ostectomy Model, Donna L. Wheeler, David L. Chamberland, John M. Schmitt, David C. Buck, John H. Brekke, Jeffrey O. Hollinger, S.-P. Joh, K.-W. Suh
Radiomorphometry And Biomechanical Assessment Of Recombinant Human Bone Morphogenetic Protein 2 And Polymer In Rabbit Radius Ostectomy Model, Donna L. Wheeler, David L. Chamberland, John M. Schmitt, David C. Buck, John H. Brekke, Jeffrey O. Hollinger, S.-P. Joh, K.-W. Suh
Faculty Publications - Department of Biological & Molecular Science
No abstract provided.
Recombinant Human Bone Morphogenetic Protein-2 And Collagen For Bone Regeneration, Jeffrey O. Hollinger, John M. Schmitt, David C. Buck, Robert Shannon, Seong-Pil Joh, H Daniel Zegzula, John Wozney
Recombinant Human Bone Morphogenetic Protein-2 And Collagen For Bone Regeneration, Jeffrey O. Hollinger, John M. Schmitt, David C. Buck, Robert Shannon, Seong-Pil Joh, H Daniel Zegzula, John Wozney
Faculty Publications - Department of Biological & Molecular Science
The study reported describes a combination of recombinant human bone morphogenetic protein-2 (rhBMP-2) and collagen (C) to regenerate bone. Unilateral critical-sized defects (CSDs) were prepared in radii of 32 skeletally mature New Zealand white rabbits. Rabbits were divided evenly among four treatments: autograft, absorbable C (Helistatt), 35 mg of rhBMP-2 combined with absorbable C (rhBMP-2/C), and untreated CSDs. The two euthanasia periods were 4 and 8 weeks. Radiographs were taken the day of surgery, every 2 weeks, and at term and the percent of radiopacity was measured. Data analysis revealed a time-dependent increase in the percent radiopacity with rhBMP-2/C. Histological …
Charged Beads Enhance Cutaneous Wound Healing In Rhesus Non-Human Primates, Elisa Burgess, Jeffrey Hollinger, Steven Bennett, John M. Schmitt, Dave Buck, Robert Shannon, Seong-Pill Joh, Jaghu Choi, Thomas Mustoe, Xuanhan Lin, Walter Skalla, Dave Connors, Chris Chrisoforou, Elliott Gruskin
Charged Beads Enhance Cutaneous Wound Healing In Rhesus Non-Human Primates, Elisa Burgess, Jeffrey Hollinger, Steven Bennett, John M. Schmitt, Dave Buck, Robert Shannon, Seong-Pill Joh, Jaghu Choi, Thomas Mustoe, Xuanhan Lin, Walter Skalla, Dave Connors, Chris Chrisoforou, Elliott Gruskin
Faculty Publications - Department of Biological & Molecular Science
Enhanced cutaneous wound healing by positively charged cross-linked diethylaminoethyl dextran beads (CLOD) was studied in a standardized incisional wound model in 20 adult and 20 geriatric 111acaca mulatta (rhesus) partitioned equally over five time periods. Physiologic saline served as a control. Soft-tissue linear incisions were prepared between and l cm inferior to the scapulae. There were four incisions per rhesus; each incision was 1.5 cm long with 1 cm of undisturbed tissue between incisions, and both the experimental CLDD and physiologic saline treatments were administered to each rhesus. The incision treatments were either CLDD and soft-tissue closure with 4-0 BioSyn …