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The Role Of The Cysteine/Glutathione Regulatory Genes Cdo1, Gsh1, And Gsh2 In Yeast-Mold Dimorphism Of The Pathogenic Fungus Histoplasma Capsulatum, Melissa Anne Adams
The Role Of The Cysteine/Glutathione Regulatory Genes Cdo1, Gsh1, And Gsh2 In Yeast-Mold Dimorphism Of The Pathogenic Fungus Histoplasma Capsulatum, Melissa Anne Adams
Dissertations
The dimorphism of Histoplasma capsulatum (Hc) from a mold to yeast is regulated by many environmental factors such as temperature and thiol concentrations. Histoplasma exists in the soil (or in vitro at 25ºC) as a multicellular saprophytic mold. In the lungs of an infected host (or in vitro at 37ºC), a shift to the unicellular parasitic yeast occurs. Sulfhydryl groups (-SH), especially cysteine, are necessary in the culture medium for the mold to yeast transition. Cysteine is the precursor for the synthesis of glutathione. Enzymes involved in the cysteine metabolism pathway are being studied in order to evaluate …
Analysis Of The Condition-Specific Regulation Of Puf3p Activity And Puf3p-Mediated Translational Repression Of Mrna In Saccharomyces Cerevisiae, Melanie A. Miller
Analysis Of The Condition-Specific Regulation Of Puf3p Activity And Puf3p-Mediated Translational Repression Of Mrna In Saccharomyces Cerevisiae, Melanie A. Miller
Dissertations
The Puf family of proteins regulates aspects of eukaryotic development such as embryonic development, and memory formation by promoting translational repression and/or degradation of targeted mRNAs in the cytoplasm. Yeast Puf3p regulates mitochondria biogenesis and function by modulating the stabilities of nuclear-transcribed mitochondrial mRNAs in response to different carbon sources. Dextrose simulates rapid Puf3p-mediated degradation of its mRNA targets via decay complex recruitment. Ethanol, galactose, or raffinose promotes stabilization of mRNA targets, as Puf3p-mediated decay activity is severely inhibited or abolished. In this work, I have established that carbon source-induced inhibition of Puf3p activity is not due to decreased transcription …
The Role Of Puf3p And Puf4p In The Regulation Of Mrna Decay In Yeast Saccharomyces Cerevisiae, Florencia Andrea Lopez Leban
The Role Of Puf3p And Puf4p In The Regulation Of Mrna Decay In Yeast Saccharomyces Cerevisiae, Florencia Andrea Lopez Leban
Dissertations
Proper regulation of gene expression at a cellular level is required in all organisms for their successful adaptation and survival to physiological or environmental changes. In eukaryotes, a convenient way of regulating gene expression is achieved by post-transcriptionally adjusting the decay rates of different mRNAs. The Puf family of proteins in yeast belong to a widespread group of eukaryotic RNA-binding proteins that regulate the lifespans of target mRNAs by sequence specifically binding to 3' untranslated regions (UTRs) and modulating their decay rates. For example, the yeast Puf3 protein binds the COX17 3' UTR, stimulating its deadenylation and subsequent decay. However, …
An Analysis Of Mer1 Function During Meiotic Splicing Regulation In Saccharomyces Cerevisiae, Frederick William Scherrer
An Analysis Of Mer1 Function During Meiotic Splicing Regulation In Saccharomyces Cerevisiae, Frederick William Scherrer
Dissertations
The transition from mitosis to meiosis in the yeast Saccharomyces cerevisiae requires a significant change to gene expression profiles. Regulation of pre-messenger RNA splicing patterns during meiosis assists in this transition by fine tuning expression of essential meiotic genes. Produced only during meiosis, Mer1p is linked to the splicing of at least three mRNAs: MER2, MER3, and AMA1. Previous evidence suggests that Mer1p activates splicing by directly recruiting snRNPs or stabilizing intermediate splicing complexes formed on pre-mRNA that contains an intronic Mer1p enhancer element. However, some splicing factors, especially accessory/non-snRNP factors, have critical roles in retaining unspliced pre-mRNAs in the …