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Consequences Of Stochastic Mrna Synthesis In A Gene Regulatory Pathway, Khyati Shah
Consequences Of Stochastic Mrna Synthesis In A Gene Regulatory Pathway, Khyati Shah
Dissertations
Gene expression is a stochastic process, with elements of randomness present in both transcription and translation. This stochasticity results in cell-to-cell variation in the amounts of gene products, mRNAs and proteins, and is observed in organisms ranging from bacteria and yeast to higher eukaryotes. Randomness in the activation and inactivation of a gene is the preliminary cause of this variation. At the level of proteins, these variations are buffered compared to levels of mRNA, due to the longer lifespan of proteins. Nevertheless, there is substantial variation observed at the level of proteins, resulting in phenotypic diversity among genetically identical cells. …
An Analysis Of Mer1 Function During Meiotic Splicing Regulation In Saccharomyces Cerevisiae, Frederick William Scherrer
An Analysis Of Mer1 Function During Meiotic Splicing Regulation In Saccharomyces Cerevisiae, Frederick William Scherrer
Dissertations
The transition from mitosis to meiosis in the yeast Saccharomyces cerevisiae requires a significant change to gene expression profiles. Regulation of pre-messenger RNA splicing patterns during meiosis assists in this transition by fine tuning expression of essential meiotic genes. Produced only during meiosis, Mer1p is linked to the splicing of at least three mRNAs: MER2, MER3, and AMA1. Previous evidence suggests that Mer1p activates splicing by directly recruiting snRNPs or stabilizing intermediate splicing complexes formed on pre-mRNA that contains an intronic Mer1p enhancer element. However, some splicing factors, especially accessory/non-snRNP factors, have critical roles in retaining unspliced pre-mRNAs in the …
Mechanism Of Splicing Regulation By The Meiosis Enhancer Factor Mer1p In Yeast Saccharomyces Cerevisiae, Javier Armisen Garrido
Mechanism Of Splicing Regulation By The Meiosis Enhancer Factor Mer1p In Yeast Saccharomyces Cerevisiae, Javier Armisen Garrido
Dissertations
In eukaryotes, genes are presented in a series of coding and non-coding DNA regions (exons/introns) that are transcribed into a premature RNA (pre-mRNA). Introns can be removed from the mature premRNA, before its translation into proteins, in a process called splicing. The splicing reaction occurs in two highly regulated transesterification reactions inside of the cell nucleus, and it is catalyzed by the Spliceosome, involving the binding and release of five small nuclear ribonucleoprotein particles (snRNPs). While some introns are constitutively spliced, others can be alternatively spliced, giving different exon combinations and therefore different proteins, increasing the protein diversity of the …