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Identification And Classification Of Coryneform Bacteria Isolated From Bovine Mammary Glands, Jeffrey L. Watts
Identification And Classification Of Coryneform Bacteria Isolated From Bovine Mammary Glands, Jeffrey L. Watts
Dissertations
Coryneform bacteria are frequently isolated from bovine mastitis and these infections are associated with economic losses. Corynehacterium bovis, a lipid requiring species, has been the most frequently isolated coryneform from the milk of infected bovine mammary glands. However, the taxonomic status of this organism is uncertain. In the current study, a polyphasic approach was used to identify coryneform bacteria isolated from bovine mastitis and determ ine the phylogenetic relationships among the identified species. A total of 212 coryneform bacteria isolated from bovine mastitis was obtained from mastitis reference laboratories in the United States and Canada. Presumptive identification based upon …
Molecular And Biological Characterization Of A Tanapox Virus Tnf-Binding Protein And The Complete Nucleotide Sequence Of The Psti-J Genomic Fragment, Mini Paulose-Murphy
Molecular And Biological Characterization Of A Tanapox Virus Tnf-Binding Protein And The Complete Nucleotide Sequence Of The Psti-J Genomic Fragment, Mini Paulose-Murphy
Dissertations
Most of the poxvirus encoded virulence factors have been identified as proteins that are secreted from infected host cells. Some of these secretory proteins impede host immune defenses. We have previously demonstrated that tanapox virus (TPV) infected cells secrete a 38 kDa glycopeptide that binds to human (h) interferon-γ, h-interleukin (IL)-2, and hIL-5. We now show an additional activity in the supernatant from TPV infected cells that down-regulates the expression of tumor necrosis factor-a (TNF-α) induced cell adhesion molecule gene expression. This activity was not detected in mock infected cells. Enzyme linked immunosorbent assays (ELISA) on primary human endothelial cells, …